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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 1003-1008.doi: 10.3724/SP.J.1006.2012.01003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Microsatellite Markers Linked to Stem Rust Resistance Gene Sr33 in Wheat

HAN Jian-Dong1,2,**,LI Wei-Hua2,**,CAO Yuan-Yin2,*,GONG Zhi-Yuan1,YAO Qiang1   

  1. 1Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2Institute of Plant Immunology, Shenyang Agricultural University, Shenyang 110866, China
  • Received:2011-09-04 Revised:2012-02-22 Online:2012-06-12 Published:2012-03-29
  • Contact: 曹远银, E-mail: caoyy66@yahoo.com.cn; Tel: 13604000358

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Abstract: Gene Sr33 confers resistance not only toUg99, but also to most Puccinia graminis f.sp. tritici races in China. A mapping population, consisting of 161 F2 plants and F2:3 families derived from the crossbetween susceptible wheat cultivar “McNair701” and a Tetra Canthatch/Triticum tauschii line carrying single Sr geneSr33, were inoculated with chinese prevalent race 34MKG to locate this resistance gene using microsatellite markers. A total of 57 microsatellite markers covering chromosome 1D were used in bulked segregation analysis (BSA) based on resistance identification at seedlings stage. Two codominant markers, Xbarc152 and Xcfd15, were obtained, which were located at both sides of Sr33. The genetic distances of Xbarc152 and Xcfd15 to the target gene were 2.4 cM and 2.1 cM respectively. These closely linked markers can be used in marker-assisted selection of wheat breeding against stem rust disease.

Key words: Microsatellite marker, Wheat stem rust, Sr33, Gene location

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