Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 1003-1008.doi: 10.3724/SP.J.1006.2012.01003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Microsatellite Markers Linked to Stem Rust Resistance Gene Sr33 in Wheat

HAN Jian-Dong1,2,**,LI Wei-Hua2,**,CAO Yuan-Yin2,*,GONG Zhi-Yuan1,YAO Qiang1   

  1. 1Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2Institute of Plant Immunology, Shenyang Agricultural University, Shenyang 110866, China
  • Received:2011-09-04 Revised:2012-02-22 Online:2012-06-12 Published:2012-03-29
  • Contact: 曹远银, E-mail: caoyy66@yahoo.com.cn; Tel: 13604000358

PDF

1347

Abstract: Gene Sr33 confers resistance not only toUg99, but also to most Puccinia graminis f.sp. tritici races in China. A mapping population, consisting of 161 F2 plants and F2:3 families derived from the crossbetween susceptible wheat cultivar “McNair701” and a Tetra Canthatch/Triticum tauschii line carrying single Sr geneSr33, were inoculated with chinese prevalent race 34MKG to locate this resistance gene using microsatellite markers. A total of 57 microsatellite markers covering chromosome 1D were used in bulked segregation analysis (BSA) based on resistance identification at seedlings stage. Two codominant markers, Xbarc152 and Xcfd15, were obtained, which were located at both sides of Sr33. The genetic distances of Xbarc152 and Xcfd15 to the target gene were 2.4 cM and 2.1 cM respectively. These closely linked markers can be used in marker-assisted selection of wheat breeding against stem rust disease.

Key words: Microsatellite marker, Wheat stem rust, Sr33, Gene location

[1] Knott D R. The Wheat Rusts: Breeding for Resistance. New York & Berlin Heidelberg: Springer-Verlag, 1989. p 201

[2] Pretorius Z A, Singh R P, Wagoire W W, Payne T S. Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f. sp. tritici in Uganda. Plant Dis, 2000, 84: 203

[3] Nazari K, Mafi M, Yahyaoui A, Singh R P, Park R F. Detection of wheat stem rust (Puccina graminis f. sp. tritici) race TTKSK (Ug99) in Iran. Plant Dis, 2009, 93: 317

[4] Tsilo T, Jin Y, Anderson J. Diagnostic microsatellite markers for detection of stem rust resistance gene Sr36 in diverse genetic backgrounds of wheat. Crop Sci, 2008, 48: 253–261

[5] McIntosh R A, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers D J, Appels R, Devos K M. Catalogue of gene symbols for wheat: MacGene 2008. Committee for the National BioResource Project (NBRP)/KOMUGI, Japan. (2009-08-27) [2011-08-29]. http://www.shigen.nig.ac.jp/wheat/komugi/genes/download.jsp.

[6] Jin Y, Singh R P, Ward R W, Wanyera R, Kinyua M. Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Puccinia graminis f. sp. tritici. Plant Dis, 2007, 91: 1096–1099

[7] Han J-D(韩建东), Cao Y-Y(曹远银), Sun Z-G(孙仲桂). Race dynamics of Puccinia graminis f. sp. tritici in China and the virulence on CIMMYT wheat germplasm resistant to Ug99. J Triticeae Crops (麦类作物学报), 2010, 30(1): 163–166 (in Chinesewith English abstract)

[8] Zhang S-S(张书绅), Qiu Y-C(邱永春), Yao P(姚平). Postulation of resistant gene to stem rust in 94 cultivars of wheat important resistant resources. J Shenyang Agric Univ (沈阳农业大学学报),1998, 29(2): 117–122 (in Chinese with English abstract)1008 作 物 学 报 第 38 卷

[9] Tsilo T J, Jin Y, Anderson J A. Microsatellite markers linked to stem rust resistance allele Sr9a in wheat. Crop Sci, 2007, 47:2013–2020

[10] Spielmeyer W, Sharp P J, Lagudah E S. Identification and validation of markers linked to broad spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.). Crop Sci, 2003, 43:333–337

[11] Parker G D, Chalmers K J, Rathjen A J, Landgridge P. Mapping loci associated with flour colour in wheat (Triticum aestivum L).Theor Appl Genet, 1998, 96: 1021–1032

[12] Tsilo T J, Chao S, Jin Y, Anderson J A. Identification and validation of SSR markers linked to the stem rust resistance gene Sr6 on the short arm of chromosome 2D in wheat. Theor Appl Genet,2009, 118: 515–524

[13] Paull J G, Pallotta M A, Landgridge P, The T T. RFLP markers associated with Sr22 and recombination between chromosome 7A of bread wheat and the diploid species Triticum boeoticum. TheorAppl Genet, 1994, 89: 1039–1045

[14] Khan R R, Bariana H S, Dholakia B B, Naik S V, Lagu M D,Rathjen A J, Bhavani S, Gupta V S. Molecular mapping of stem and leaf rust resistance in wheat. Theor Appl Genet, 2005, 111: 846–850

[15] Han J-D(韩建东), Zhu G-Q(朱桂清), Li W-H(李伟华), Cao Y-Y(曹远银). New SSR markers for stem rust resistance gene Sr22 in wheat. Sci Agric Sin (中国农业科学), 2010, 43(15):3244–3250 (in Chinese with English abstract)

[16] Mago R, Bariana H S, Dundas I A, Spielmeyer W, Lawrence G J, Pryor A J, Elli J G. Development of PCR markers for the selection of wheat stem rust resistance genes Sr24 and Sr26 in diverse wheat germplasm. Theor Appl Genet, 2005, 111: 496–504

[17] Prins R, Groenewald J Z, Marias G F, Snape J W, Koebner R M D. AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor Appl Genet, 2001, 103: 618–624

[18] Das B K, Saini A, Bhagwa S G, Jawali N. Development of SCAR markers for identification of stem rust resistance gene Sr31 in the homozygous or heterozygous condition in bread wheat. Plant Breed, 2006, 125: 544–549

[19] Helguera M, Khan I A, Kolmer J, Lijavetzky D, Zhong-qi L, Dubcovsky J. PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines. Crop Sci, 2003, 43: 1839–1847

[20] Gold J, Harder D, Townley-Smith F, Aung T, Procunier J. Development of a molecular marker for rust resistance gene Sr39 and Lr35 in wheat breeding lines. Electronic J Biotechnol, 1999,2: 35–40

[21] Mater Y, Baenzinger S, Gill K, Graybosch R, Whitcher L, Baker C, Specht J, Dweikat I. Linkage mapping of powdery mildew and greenbug resistance genes on recombinant 1RS from ‘Amigo’ and ‘Kaukaz’ wheat-rye translocations of chromosome 1RS·1AL.Genome, 2004, 47: 292–298

[22] Mago R, Spielmeyer W, Lawrence G, Lagudah E, Ellis J, Pryor A. Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theor Appl Genet, 2002, 104:1317–1324

[23] Mago R, Spielmeyer W, Lawrence G J, Ellis J G, Prior A J. Resistance genes for rye stem rust (SrR) and barley powdery mildew (Mla) are located in syntenic regions on short arm of chromosome.Genome, 2004, 47: 112–121

[24] Roelfs A P. Race specificity and methods of study. Cereal Rust,1985, (1): 131–164

[25] Aldrich J, Cullis C A. RAPD analysis in flax: optimization of yield and reproducibility using Klen Taq l DNA polymerase, Chelex 100, and gel purification of genomic DNA. Plant Mol Biol Rep, 1993, 11: 128–141

[26] Michelmore R W, Paran I. Identification of markers linked to disease-resistance genes by bulked segregate analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Nat Acad Sci USA, 1991, 88:9828–9832

[27] Röder M S, Korzun V, Wendehake K, Plaschke J, Tixier M H, Leroy P, Ganal M W. A microsatellite map of wheat. Genetics,1998, 149: 2007–2023

[28] Bassam B J, Caetano-Anollés G, Gresshoff P M. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem,1991, 196: 80–83

[29] Lagudah E S, Halloran G M. Phylogenetic relationship of Triticum tauschii, the D genome donor to hexaploid wheat: I. Variation in HMW subunits of glutenin and gliadins. Theor Appl Genet,1988, 75: 592–598

[30] McIntosh R A. A catalogue of gene symbols for wheat. In: Miller T E, Koebner R M D, eds. Proc 7th Int Wheat Genet Symp IPSRCambridge, 1988. pp 1225–1324

[31] McIntosh R A, Hart G E, Devos K M, Gale M D, Rogers W J. Catalogue of gene symbols for wheat. In: Slinkard A E ed. Proc 9th Intl Wheat Genet Symp (Vol. 5). University of Saskatchewan, Saskatoon, Canada: University Extension Press, 1998. pp 1–235

[32] Kerber E R, Dyck P L. Resistance of stem and leaf rust of wheat in Aegilops squarrosa and transfer of a gene for stem rust resistance to hexaploid wheat. In: Proc 5th Int Wheat Genet Symp, New Delhi, India, 1979. pp 358–364

[33] Jones S S, Dvorak J, Knot D R, Qualset C O. Use of doubleditelosomic and normal chromosome 1D recombinant substitution lines to map Sr33 on chromosome arm 1DS in wheat. Genome,1991, 34: 505–508

[34] Somers D J, Isaac P, Edwards K. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet, 2004, 109: 1105–111
[1] CHENG Xin,REN De-Yong,MA Jiao,ZHU Xiao-Yan,SANG Xian-Chun,LING Ying-Hua,ZHAO Fang-Ming,HE Guang-Hua*. Identification and Gene Mapping of Leaf Pale Yellow-Revertible Mutant pyr1 in Rice [J]. Acta Agron Sin, 2013, 39(06): 992-998.
[2] ZHANG Jia-Jia,YUAN Hong-Xia,ZHANG Rui-Qi,XING Xiao-Ping,DAI Jun-Li,NIU Ji-Shan,LI Hong-Lian,CHEN Pei-Du. Analysis of Resistance to Heterodera filipjevi in Triticum aestivum–Dasypyrum villosum Germplasm [J]. Acta Agron Sin, 2012, 38(11): 1969-1976.
[3] CHEN Dai-Bo, ZHAN Xiao-Ding, WU Chao, SHEN Xi-Hong, WU Wei-Ming, GAO Zhi-Qiang, CHENG Shi-Hua, CAO Li-Yong. Genetic Analysis and Gene Mapping of a Long Empty Glumes Mutant in Rice (Oryza sativa L.) [J]. Acta Agron Sin, 2010, 36(09): 1506-1511.
[4] QI Dong-Ling,GUO Gui-Zhen,LEE Myung-Chul,YANG Chun-Gang,ZHANG Jun-Guo,CAO Gui-Lan,ZHANG San-Yuan,SUH Seok-Cheol,ZHOU Qing-Yang,HAN Long-Zhi. Identification of Quantitative Trait Loci for Alkaline Tolerance at Early Seedling Stage under Alkaline Stress in Japonica Rice [J]. Acta Agron Sin, 2009, 35(2): 301-308.
[5] LIAO Xiang-Zheng;WANG Jin;ZHOU Rong-Hua;REN Zheng-Long;JIA Ji-Zeng. Mining Favorable Alleles of QTLs Conferring 1000-Grain Weight from Synthetic Wheat [J]. Acta Agron Sin, 2008, 34(11): 1877-1884.
[6] CHEN Hua-Hua; LI Jun;WAN Hong-Shen;WANG Ling-Ling;PENG Zheng-Song;YANG Wu-Yun. Microsatellite Markers for Culm Wall Thickness and Anatomical Features of Solid Stem Wheat 86-741 [J]. Acta Agron Sin, 2008, 34(08): 1381-1385.
[7] ZHANG Yuan-Yuan;CAO Gui-Lan;HAN Long-Zhi. Analysis of Relationships for Indica Local Rice from Different Places of China [J]. Acta Agron Sin, 2007, 33(05): 757-762.
[8] SONG Zhen-Yun ;YANG Zhi-Min ; ; CHEN Xu-Sheng ;. Gene Mapping of a Sub-Red Mutant in Upland Cotton [J]. Acta Agron Sin, 2007, 33(03): 511-513.
[9] ZHU Zhen-Dong;HUO Yun-Long;WANG Xiao-Ming;HUANG Jun-Bin;WU Xiao-Fei. Molecular Identification of a Novel Phytophthora Resistance Gene in Soybean [J]. Acta Agron Sin, 2007, 33(01): 154-157.
[10] MA Qiang;LUO Pei-Gao;REN Zheng-Long;JIANG Hua-Ren;YANG Zu-Jun. Genetic Analysis and Chromosomal Location of Two New Genes for Resistance to Powdery Mildew in Wheat (Triticum aestivum L.) [J]. Acta Agron Sin, 2007, 33(01): 1-8.
[11] WANG Chang-You;JI Wan-Quan;ZHANG Gai-Sheng;WANG Qiu-Ying;CAI Dong-Ming;XUE Xiu-Zhuang. SSR Markers and Preliminary Chromosomal Location of a Powdery Mildew Resistance Gene in Common Wheat Germplasm N9134 [J]. Acta Agron Sin, 2007, 33(01): 163-166.
[12] CHENG Ying;SONG Wei;LIU Zhi-Yong;XIE Chao-Jie;NI Zhong-Fu;PENG Hui-Ru;NIE Xiu-Ling;YANG Zuo-Min;SUN Qi-Xin. Microsatellite Markers for A Yellow Rust Resistant Gene in Wheat Cultivar Guinong 21 [J]. Acta Agron Sin, 2006, 32(12): 1867-1872.
[13] HAN Long-Zhi ;ZHANG San-Yuan;QIAO Yong-Li ;KIM Jong-Hwan;XU Fu-Rong;CAO Gui-Lan;NAN Zhong-Hao;DAI Lu-Yuan;YEA Jong-Doo;OH Hee-Jong. Identification of QTLs for Seed Setting Rate in Rice under Different Growing Environments [J]. Acta Agron Sin, 2006, 32(07): 1024-1030.
[14] SUI Jiong-Ming;LIANG Guo-Hua;LI Xin;WU Shu-Jun;YAN Song;ZHAO Xiang-Qiang; ZHANG Zheng-Qiu; GU Ming-Hong. Genetic Analysis and Gene Location of a Semidwarf Gene in an Indica Rice Cultivar Duonieai (Oryza sativa L.) [J]. Acta Agron Sin, 2006, 32(06): 845-850.
[15] LIU Bin-Mei; WU Yue-Jin; TONG Ji-Ping; WU Jin-De; YU Zeng-Liang; ZHANG Ying and CHENG Can. SCAR Markers and Preliminary Gene Mapping of a Dominant Semi-dwarf Mutant in Rice [J]. Acta Agron Sin, 2006, 32(03): 449-454.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd