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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (02): 207-213.doi: 10.3724/SP.J.1006.2015.00207

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Cytogenetic Characterization of Triticum–Psathyrostachys Substitution Line DH2322

WANG Xiu-Juan,CHEN Xin-Hong,PANG Yu-Hui,JING Fan,ZHANG Jun,HU Si-Yuan,ZAN Kai,WU Jun,YANG Qun-Hui,ZHAO Ji-Xin *   

  1. College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China
  • Received:2014-06-09 Revised:2014-12-19 Online:2015-02-12 Published:2014-12-29
  • Contact: 赵继新, E-mail: zhjx881@163.com E-mail:wangxiujuanluck@163.com

Abstract:

A wheat–P. huashanica alien substitution line DH2322 was isolated from the F4 progeny of heptaploid hybrid H8911 × Triticum durum cv. D4286 and identified by using SCAR markers, genomic in situ hybridization (GISH) , SSR markers, and STS markers. The rusults using SCAR marker RHS141 showeded that DH2322 carrys chromosome derived from P. huashanica. Cytogenetic observation indicated that DH2322 has a chromosome karyotype of 2n = 42 = 21 II. GISH showed that DH2322 is a line with 40 chromosomes from wheat and two Ns chromosomes from P. huashanica, which formed a ring bivalent in PMC at metaphase I. Analysis with SSR and STS primers showed that the wheat chrom osome 2D in DH2322 was substituted by the chromosome 2Ns from P. huashanica. Evaluation of the agronomic traits showed that DH2322 presented favorable agronomic traits in spike length and kernels per spike. Development of this new germplasm will be useful for wheat breeding programs in the future.

Key words: Triticum aestivum, Psathyrostachys huashanica, Alien substitution line, SCAR, GISH, SSR, STS

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