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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (9): 1729-1737.doi: 10.3724/SP.J.1006.2009.01729

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

QTL Mining for Sheath Blight Resistance Using the Backcross Selected Introgression Lines for Grain Quality in Rice

LI Fang1,CHENG Li-Rui1,XU Mei-Rong1,ZHOU Zheng1,ZHANG Fan1,SUN Yong1,ZHOU Yong-Li1,ZHU Ling-Hua1,XU Jian-LOng1*,LI Zhi-Kang1,2   

  1. 1Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2International Rice Research Institute,DAPO Box7777,Metro Manila,Philippines
  • Received:2009-02-05 Revised:2009-03-20 Online:2009-09-12 Published:2009-07-04
  • Contact: XU Jian-ling,E-mail: xujl@caas.net.cn


QTLs for disease scale (DS), relative lesion height (RH) and plant height (PH) were mapped using the four introgression lines selected against grain quality from the four introgression populations between two elite varieties, IR64 and Teqing as recurrent parents and two tall varieties, Tarom Molaii and Binam with high grain quality and sheath blight resistance (SBR) as donors. DS had high significant positive correlation with RH, and both of them were significantly negatively correlated with PH. DS, RH and PH presented wide segregations even in the relative small (2860) introgression populations, including some semidwarf plants segregated with overparent SBR. Ten, eight, eight and six QTLs for the three traits were identified in IR64/Tarom Molaii, Teqing/Tarom molaii, IR64/Binam and Teqing/Binam, respectively by one   way ANOVA analysis. The two donor alleles at most QTLs reduced DS and RH as well as increased PH. No any a common QTL for each of the three traits was detected in the populations of the same donor in the two different background, indicating there was a obvious genetic background effect on expression of SBR- and PH-QTLs. QTLs linked to the same marker loci between PH and DS, and PH and RH accounted for 38% and 52% of total QTLs for the two traits, respectively, indicating SBR had tight relationship with PH and both of them shared some linked loci. As compared with the previous QTL mapping results of grain quality in the same populations, most QTLs for grain quality and SBR distributed in different chromosome regions and showed independently inherited. Efficiency, influencing factors and its application in breeding of QTL mapping for non-target traits using introgression lines selected against the target trait were deeply discussed, and importance of selective introgression lines in QTL mining and breeding use was also emphasized.

Key words: Backcross selected introgression lines(SBILs), Quantiative trait locus(QTL), Rice sheath blight resistance(SBR), Grain quality, Plant height

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