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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (09): 1582-1593.doi: 10.3724/SP.J.1006.2013.01582

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Developing Restorer Lines Pyramiding Different Resistant Genes to Blast and Bacterial Leaf Blight by Marker-assisted Selection in Rice

PAN Xiao-Biao1,**,CHEN Kai2,3,**,ZHANG Qiang2,3,HUANG Shan-Jun1,XIE Liu-Jie1,LI Mei4,Meng Li-Jun2,4,XU Zheng-Jin3,XU Jian-Long2,4,*,LI Zhi-Kang2,4   

  1. 1 Taizhou Academy of Agricultural Sciences of Zhejiang Province, Linhai 317000, China; 2 Institute of Crop Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Shenyang Agricultural University / Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Shenyang 110161, China; 4 Shenzhen Institute of Biological Breeding & Innovation, Chinese Academy of Agricultural Sciences, Shenzhen 518083, China?
  • Received:2013-01-14 Revised:2013-06-04 Online:2013-09-12 Published:2013-07-09

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Abstract:

The novel blast resistance genes, Pi-GD-1(t), Pi-GD-2(t), and GLP8-6(t) from rice variety Sanhuangzhan2 (here abbreviated them as G1, G2 and G8, respectively) and bacterial blight (BB) resistance gene, Xa23 were introgressed into three elite restorer lines (Minghui86, Shuhui527 and Zhehui7954) by marker-assisted pyramiding breeding approaches in combination with artificial inoculation and stringent phenotypic selections. The five derived blast and bacterial blight resistance restorer lines, Minghui86-G1-G2-Xa23, Shuhui527-G2-Xa23, Mingzhe-G2-G8-Xa23-1, Mingzhe-G2-G8-Xa23-2 and Mingzhe-G1-G2-G8-Xa23, demonstrated similar or wider blast resistance spectrum as compared with the donor parent, Sanhuangzhan 2. The five derived BB resistant restorer lines and their derived hybrid combinations with II-32A demonstrated similar BB resistance spectrum to the donor parent, IRBB23. The newly developed BB resistant restorers and their derived hybrids were identical to their respective original versions for agronomic traits especially under disease free condition. However, under severe disease condition, the five BB resistant restorer lines exhibited significantly higher grain weight, spikelet fertility and grain yield as compared with the respective original restorer lines, thus further resulting in significantly higher grain yields in BB resistant hybrids than in their respective original hybrids. Improvement of resistance of blast and BB for restorer lines by molecular marker-assisted pyramiding was discussed.

Key words: Rice restorer line, Blast, Bacterial leaf blight, Resistance improvement, Marker assisted selection (MAS)

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