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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (01): 54-62.doi: 10.3724/SP.J.1006.2014.00054

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification, Genetic Analysis and Gene Mapping of a Rice Blast Resistance Gene in Japonica Rice

LI Bin1,DENG Yuan-Bao1,YAN Xue-Hai1,YANG Yang1,LIU Peng-Qiang1,DU Yong1,XIE Pei1,WANG De-Zheng2,DENG Qi-Ming1,*,LI Ping1,*   

  1. 1 Rice Research Institute of Sichuan Agricultural University, Wenjiang 611130, China; 2 Anhui Academy of Agricultural Sciences, Hefei 230031, China?
  • Received:2013-05-08 Revised:2013-06-16 Online:2014-01-12 Published:2013-10-22
  • Contact: 邓其明, E-mail: dengqmsc@163.com, Tel: 1388065608; 李平, E-mail: liping6575@163.com, Tel: 13908070452 E-mail:libin15999211540@sina.com

Abstract:

7001S is a male-sterile rice with broad-spectrum resistance to rice blast pathogens and highly resistant to 22 strains of Magnaporthe oryzae (M. oryzae). The F2 generation of hybrid between 7001S and 80-4B showed significant resistance to rice blast pathogens. The ratio of resistant plants: susceptible plants was 3:1, indicating that the resistance of 7001S to the rice blast is controlled by one-dominant karyogene or a QTL locus. Molecular marker analysis showed that the rice blast resistance gene was located on the terminal long arm of chromosome 11 between P21-2415 and RM27322, with genetic distance of 0.27 cM and physical distance of 310 kb. Some co-segregated molecular markers were also found in this gene area and could be used for identifying candidate genes.

Key words: Rice blast, 7001S, Resistance gene, Molecular markers, Genetic analysis, Gene mapping

[1]Moffat A S. Plant genetics. Mapping the sequence of disease resistance. Science, 1994, 265: 1804–1805



[2]Skamnioti P, Gurr S J. Against the grain: safeguarding rice from rice blast disease. Trends Biotechnol, 2009, 27: 141–150



[3]赵国珍, 贾育林, 严宗卜, Christopher WDEREN, Melissa H JIA, 戴陆园. 一种高效便捷的水稻DNA提取法及其应用. 中国水稻科学. 2012, 26: 495–499



Zhao G-Z, Jia Y L, Yan Z B, Christopher WDEREN, Melissa H JIA, Dai L G. An efficient, economic, and rapid rice DNA extracton method and its application. Chin J Rice Sci, 2012, 26: 495–499 (in Chinese with English abstract)



[4]雷财林, 凌忠专, 王久林. 水稻抗病育种研究进展. 生物学通报, 2004, 39(11): 4–7



Lei C L, Ling Z Z, Wang J L. Research advanccs in rice breeding for disease resistance. Bull Biol, 2004, 39(11): 4–7 (in Chinese)



[5]Ashikawa I, Hayashi N, Yamane H, Kanamori H, Wu J Z, Matsumoto T, Ono K, Yano M. Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance. Genetics, 2008, 180: 2267–2276



[6]Bryan G T, Wu K S, Farrall L, Jia Y L, Hershey H P, McAdams S A, Faulk K N, Donaldson G K, Tarchini R, Valent B. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta. Plant Cell, 2000, 12: 2033–2046



[7]Hua L, Wu J, Chen C, Chen C X, Wu W H, He X Y, Lin F, Wang L, Ashikawa I, Matsumoto T, Wang L, Pan Q H. The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast. Theor Appl Genet, 2012, 125: 1047–1055



[8]Kanzaki H, Yoshida K, Saitoh H, Fujisaki K, Hirabuchi A, Alaux L, Fournier E, Tharreau D, Terauchi R. Arms race co-evolution of Magnaporthe oryzae AVR-Pik and rice Pik genes driven by their physical interactions. Plant J, 2012, 72: 894–907



[9]Lee S W, Han S W, Sririyanum M, Park CH J, Seo Y S, Ronald P C. A type I-secreted, sulfated peptide triggers XA21-mediated innate immunity. Science, 2009, 326: 850–853



[10]Lin F, Chen S, Que Z, Wang L, Liu X Q, Pan Q H. The blast resistance gene Pi37 encodes a nucleotide binding site leucine-rich repeat protein and is a member of a resistance gene cluster on rice chromosome 1. Genetics, 2007, 177: 1871–1880



[11]Liu J, Liu X, Dai L, Wang G L. Recent progress in elucidating the structure, function and evolution of disease resistance genes in plants. J Genet Genomics, 2007, 34: 765–776



[12]Qu S D, Liu G F, Zhou B, Bellizzi M, Zeng L R, Dai L Y, Wang G L. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. Genetics, 2006, 172: 1901–1914



[13]Wang W, Wen Y, Berkey R, Xiao S Y. Specific targeting of the Arabidopsis resistance protein RPW8.2 to the interfacial membrane encasing the fungal Haustorium renders broad-spectrum resistance to powdery mildew. Plant Cell, 2009, 21: 2898–2913



[14]Xu Y B. Molecular Plant Breeding. USA: International Institute for Applied Biological Science Center, 2012. pp 213–219



[15]Zhou B, Qu S, Liu G, Dolan M, Sakai H, Lu G D, Bellizzi M, Wang G. The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea. Mol Plant Microbe Interact, 2006, 19: 1216–1228



[16]Zhu X, Chen S, Yang J, Zhou S C, Zeng L X, Han J L, Su J, Wang L, Pan Q H. The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family. Theor Appl Genet, 2012, 124: 1295–1304



[17]Fukuoka S, Saka N, Koga H, Ono K, Shimizu T, Ebana K, Hayashi N, Takahashi A, Hirochika H, Okuno K, Yano M. Loss of function of a proline-containing protein confers durable disease resistance in rice. Science, 2009, 325: 998–1001



[18]Hayashi N, Inoue H, Kato T, Funao T, Shirota M, Shimizu T, Kanamori H, Yamane H, Saito Y H, Matsumoto T, Yano M, Takatsuji H. Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication. Plant J, 2010, 64: 498–510



[19]Chen X, Shang J, Chen D, Lei C L, Xu J C, Ling Z Z, Cao G, Ma B T, Wang Y P, Zhao X F, Li S G, Zhu L H. A B-lectin receptor kinase gene conferring rice blast resistance. Plant J, 2006, 46: 794–804



[20]刘华招, 陈温福, 刘延. 水稻基因分子标记的物理图谱锚定. 华北农学报, 2009, 24(增刊): 5–8



Liu H-Z, Chen W-F, Liu Y. Rice Pi genes molecular markers anchored to the physics map of rice genome. Acta Agric Boreali-Sin, 2009, 24(suppl): 5–8 (in Chinese with English abstract)



[21]Zhai C, Lin F, Dong Z, He X Y, Yuan B, Zeng X S, Wang L, Pan Q H. The isolation and characterization of Pik, a rice blast resistance gene which emerged after rice domestication. New Phytol, 2011, 189: 321–334



[22]Yuan B, Zhai C, Wang W, Zeng X S, Xu X K, Hu H Q, Lin F, Wang L, Pan Q H. The Pik-p resistance to Magnaporthe oryzae in rice is mediated by a pair of closely linked CC-NBS-LRR genes. Theor Appl Genet, 2011, 122: 1017–1028



[23]Okuyama Y, Kanzaki H, Abe A, Yoshida K, Tamiru M, Saitoh H, Fujibe T, Matsumura H, Shenton M, Galam D C, Undan J, Ito A, Sone T, Terauchi R. A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes. Plant J, 2011, 66: 467–479



[24]Bamshad M, Wooding S P. Signature of natural selection in the human genome. Nat Rev Genet, 2003, 4: 99–111



[25]Tian D, Araki H, Stahl E, Bergelson J, Kreitman M. Signature of balancing selection in the Arabidopsis. Proc Natl Acad Sci USA, 2002, 99: 11525–11530



[26]Brunner S, Hurni S, Streckeisen P, Mayr G, Albrecht M, Yahiaoui N, Keller B. Intragenic allele pyramiding combines different specificities of wheat Pm3 resistance alleles. Plant J, 2010, 64: 433–445



[27]Ravensdale M, Nemri A, Thrall P H, Ellis J G, Dodds P N. Co-evolutionary interactions between host resistance and pathogen effector genes in flax rust disease. Mol Plant Pathol, 2011, 12: 93–102



[28]Rai A K, Kumar S P, Gupta S K, Gautam N, Singh N K, Sharma T R. Functional complementation of rice blast resistance gene Pi-K(H)(Pi54) conferring resistance to diverse strains of Magnaporthe oryzae. Plant Biochem Biotechnol, 2011, 20: 55–65



[29]李成云, 陈宗麒, 陈琼珠,稻瘟病菌的研究进展. 西南农业学报, 1995, 8(3): 107–112



Li C Y, Chen Z Q, Chen Q Z. Research progress of rice blast fungus. Southwest China J Agric Sci, 1995, 8(3): 107–112 (in Chinese)



[30]Takahashi A, Hayashi N, Miyao A, Hirochika H. Unique features of the rice blast resistance Pi-sh locus revealed by large scale retrotransposon-tagging. BMC Plant Biol, 2010, 10(175): 1–14



[31]刘海, 肖应辉, 唐文邦, 邓化冰, 陈立云. 水稻两用核不育系繁殖基地计算机选择系统研制与应用. 作物学报, 2011, 37: 755–763



Liu , Xiao Y H, Tang W B, Deng H B, Chen L Y. Development and application of a computer-aided selection system for thermo-sensitive genic male sterile rice multiplying site. Acta Agron Sin, 2011, 37: 755–763 (in Chinese with English abstract)



[32]杨仕华, 程本义, 沈伟峰, 夏俊辉. 中国两系杂交水稻选育与应用进展. 杂交水稻, 2009, 24(1): 5–9



Yang S H, Cheng B Y, Shen W F, Xia J H. Progress of application and breeding on two-line hybrid rice in China. Hybrid Rice, 2009, 24(1): 5–9 (in Chinese with English abstract)

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