Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (01): 31-42.doi: 10.3724/SP.J.1006.2016.00031


Evaluation of Improvement Effect of Restorer Lines on Pyramiding Genes Resistant to Rice Blast, Bacterial Leaf Blight and Brown Planthopper

LOU Jue1,YANG Wen-Qing1,LI Zhong-Xing2,LUO Tian-Kuan1,XIE Yong-Chu1,ZHENG Guo-Chu1,YUE Gao-Hong1,XU Jian-Long3,LU Hua-Jin1,*   

  1. 1 Southern Zhejiang Key Laboratory of Crop Breeding, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, China; 2 Wenzhou Plant Protection Station, Wenzhou 325000, China; 3 Institute of Crop Sciences/ National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2015-06-12 Revised:2015-09-06 Online:2016-01-12 Published:2015-10-13
  • Contact: 卢华金, E-mail: luhjzw@163.com E-mail:yyljlu@wzvcst.edu.cn
  • Supported by:

    This research was supported by Special Projects of the Zhejiang Agricultural Key Research (8812 Plan, 2012C12901-17) and Wenzhou Seeds and Seedlings Technology Innovation (N20120019)), Project of the Zhejiang Education Science and Planning (2015SCG183) and General Research Program for the Education Department of Zhejiang Province (Y201533916).


The novel blast resistance genes, Pi-GD-1(t) and Pi-GD-2(t) (abbreviated as G1 and G2, respectively) from rice cultivar Sanhuangzhan2, bacterial blight resistance gene Xa23 (abbreviated as X) from CBB23 and brown planthopper resistance gene Bph18(t) (abbreviated as B) from IR65482-7-216-1-2-B (abbreviated as IR65482) were introgressed into three rice restorer lines, Wenhui 845, Wenhui 117 and Wenhui 143, by marker-assisted backcross and pyramiding breeding methods integrated with artificial inoculation, natural induction and phenotypic selections. The eight resistant restorer lines pyramiding blast and brown planthopper resistance genes, i.e. Wenhui 845-G1-G2-B-4, Wenhui 845-G1-G2-B-5, Wenhui 117-G1-G2-X-B-3, Wenhui 143-G1-G2-B-3, Wenhui 143-G2-X-B-9, Wenhui 143-G2-X-B-10, Wenhui 143-G1-G2-B-11, and Wenhui 143-G1-G2-B-37, and their tester lines crossed with the sterile line Wufeng A demonstrated the similar or slight lower level of resistance against rice blast and brown planthopper as compared with the resistant donor parents Sanhuangzhan2 or IR65482. Part of developed restorer lines (Wenhui 117-G1-G2-X-B-3, Wenhui 143-G2-X-B-9 and Wenhui 143-G2-X-B-10) and their tester lines showed resistance or moderate resistance to bacterial blight. The newly developed restorers resistant to blast, bacterial blight and brown planthopper were similar or superior to their respective original types in agronomic traits under normal condition, implying these resistant restorer lines can be useful in hybrid rice breeding and production. The results indicated that Xa23 could completely express its dominant resistance against different restorer genetic backgrounds whereas effects of resistance improvement in Pi-GD-1(t), Pi-GD-2(t), and Bph18(t) depend on their genetic background.

Key words: Rice restorer lines, Blast, Bacterial blight, Brown planthopper, Resistance improvement, Marker-assisted selection

[1]Dean R A, Talbot N J, Ebbole D J, Farman M L, Mitchell T K, Orbach M J, Thon M, Kulkarni R, Xu J R, Pan H, Read N D, Lee Y H, Carbone I, Brown D, Oh Y Y, Donofrio N, Jeonq J S, Soanes D M, Djonovic S, Kolomiets E, Rehmeyer C, Li W, Harding M, Kim S, Lebrun M H, Bohnert H, Coughlan S, Butler J, Calvo S, Ma L J, Nicol R, Purcell S, Nusbaum C, Galagan J E, Birren B W. The genome sequence of the rice blast fungus Magnaporthe grisea. Nature, 2005, 434: 980–986

[2]Singh G P, Srivastava M K, Singh R V, Singh R M. Variation in quantitative and qualitative losses caused by bacterial blight in different rice varieties. Indian Phytopathol, 1997, 30: 180–185

[3]Mew T W. Current status and future prospects of research on bacterial blight of rice. Ann Rev Phytopathol, 1987, 25: 359–382

[4]张珏锋, 吴鸿, 陈建明, 郑许松, 陈列忠, 俞晓平. 一株褐飞虱内共生菌的分离及分子鉴定. 中国水稻科学, 2007, 21: 551–554

Zhang J F, Wu H, Chen J M, Zheng X S, Chen L Z, Yu X P. A strain isolated from brown planthopper and its molecular identification. Chin J Rice Sci, 2007, 21: 551–554 (in Chinese with English abstract)

[5]全国农业技术推广服务中心. 2011年全国农作物主要品种推广情况统计表. 北京: 2011. pp 10–25

National Agricultural Technology Extension Service Center. Statistics on the Promotion of the Main Varieties of Crops in 2011. Beijing: 2011. pp 10–25 (in Chinese)

[6]沈瑛, 朱培良, 袁筱萍, Levy M, Decker M, Talbot N, Hamer J E. 中国稻瘟病菌的遗传多样性. 植物病理学报, 1993, 23(4): 309–313

Shen Y, Zhu P L, Yuan X P, Levy M, Decker M, Talbot N, Hamer J E. Genetic diversity of rice blast fungus in China. Acta Phytopathol Sin, 1993, 23(4): 309–313 (in Chinese with English abstract)

[7]Mew T W, Vera Cruz C M, Medalla E S. Changes in race frequency of Xanthomonas Oryzae pv. oryzae in response to rice cultivars planted in the Philippines. Plant Dis, 1992, 76: 1029–1032

[8]闫成业, Mamadou G, 朱子建, 牟同敏. 分子标记辅助选择改良水稻恢复系R1005的褐飞虱抗性. 华中农业大学学报, 2014, 33(5): 8-14

Yan C Y, Mamadou G, Zhu Z J, Mou T M. Improving the brown planthopper resistance of rice restorer line R1005 with molecular marker-assisted selection. J Huazhong Agric Univ, 2014, 33(5): 8–14 (in Chinese with English abstract)

[9]柏斌, 吴俊, 周波, 邓启云. 稻瘟病抗性分子育种研究综述. 杂交水稻, 2012, 27(3): 5–9

Bai B, Wu J, Zhou B, Deng Q Y. Research progress of molecular breeding of rice blast resistance. Hybrid Rice, 2012, 27(3): 5–9 (in Chinese with English abstract)

[10]伍尚忠, 朱小源, 刘斌, 杨祁云, 张少红, Leung H. 籼稻品种三黄占2号的稻瘟病持久抗性评价与遗传分析. 中国农业科学, 2004, 37: 528–534

Wu S Z, Zhu X Y, Liu B, Yang Q Y, Zhang S H, Leung H. Genetic analysis and evaluation of durable resistance to blast in Indica cultivar Sanhuangzhan 2. Sci Agric Sin, 2004, 37: 528–534 (in Chinese with English abstract)

[11]Liu B, Zhang S H, Zhu X Y, Yang Q Y, Wu S Z, Mei M T, Mauleon R, Leach J, Mew T, Leung H. Candidate defense genes as predictors of quantitative blast resistance in rice. Mol Plant Microbe Interact, 2004, 17: 1146–1152

[12]许美荣, 周永力, 黎志康. 水稻对白叶枯病和细菌性条斑病的抗性遗传研究进展. 植物遗传资源学报, 2012, 13: 370–375

Xu M R, Zhou Y L, Li Z K. Research advances in on resistance genetics of rice bacterial blight and bacterial leaf streak. J Plant Genet Resour, 2012, 13: 370–375 (in Chinese with English abstract)

[13]潘海军, 王春连, 赵开军, 章琦, 樊颖伦, 周少川, 朱立煌. 水稻抗白叶枯病基因Xa23的PCR分子标记定位及辅助选择. 作物学报, 2003, 29: 501–507

Pan H J, Wang C L, Zhao K J, Zhang Q, Fan Y L, Zhou S C, Zhu L H. Molecular mapping by PCR-based markers and marker-assisted selection of Xa23, a bacterial blight resistance gene in rice. Acta Agron Sin, 2003, 29: 501–507 (in Chinese with English abstract)

[14]梁云涛, 王春连, 刘丕庆, 傅强, Kshirod K J, 赵开军. 水稻抗褐飞虱基因研究和利用现状. 植物遗传资源学报, 2008, 9: 119–124

Liang Y T, Wang C L, Liu P Q, Fu Q, Kshirod K J, Zhao K J. Research and application status of rice genes resistance to brown planthopper. J Plant Genet Resour, 2008, 9: 119–124 (in Chinese with English abstract)

[15]Rahman M L, Jiang W Z, Chu S H, Qiao Y L, Ham T H, Woo M O, Lee J Y, Khanam M S, Chin J H, Jeung J U, Brar D S, Jena K K, Koh H J. High-resolution mapping of two rice brown plantbpper resistance genes, Bph20(t) and Bph21(t), originating from Oryza minula. Theor Appl Genet, 2009, 119: 1237–1246

[16]梁云涛, 王春连, 赖凤香, 刘丕庆, 王坚, 傅强, 赵开军. 水稻抗褐飞虱基因Bph18(t)的STS标记开发及有效性验证. 中国水稻科学, 2010, 24: 244–250

Liang Y T, Wang C L, Lai F X, Liu P Q, Wang J, Fu Q, Zhao K J. Development of STS marker KCl for brown planthopper resistance gene Bph18(t) and marker assisted selection efficiency. Chin J Rice Sci, 2010, 24: 244–250 (in Chinese with English abstract)

[17]Jena K K, Jeung J U, Lee J H, Choi H C, Brar D S. High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t), and marker-assisted selection for BPH resistance in rice (Oryza sativa L.). Theor Appl Genet, 2006, 112: 288–297

[18]倪大虎, 易成新, 李莉, 汪秀峰, 张毅, 赵开军, 王春连, 章琦, 王文相, 杨剑波. 分子标记辅助培育水稻抗白叶枯病和稻瘟病三基因聚合系. 作物学报, 2008, 34(1): 100–105

Ni D H, Yi C X, Li L, Wang X F, Zhang Y, Zhao K J, Wang C L, Zhang Q, Wang W X, Yang J B. Development rice lines resistance to bacterial blight and blast with molecular marker-assisted selection. Acta Agron Sin, 2008, 34(1): 100–105 (in Chinese with English abstract)

[19]中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. GB/T 15790-2009, 稻瘟病测报调查规范. 北京: 中国标准出版社, 2009

General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, China National Standardization Management Committee. GB/T 15790-2009, Rules of investigation and forecast for the rice blast [Pyricularia oryzae (Cavara)]. Beijing: Standard Press of China, 2009 (in Chinese)

[20]刘光杰, 付志红, 沈君辉, 张亚辉. 水稻品种对稻飞虱抗性鉴定方法的比较研究. 中国水稻科学, 2002, 16: 52–56

Liu G J, Fu Z H, Shen J H, Zhang Y H. Comparative study on evaluation methods for resistance to rice planthoppers (Homoptera: Delphacidae) in rice. Chin J Rice Sci, 2002, 16: 52–56 (in Chinese with English abstract)

[21]陈凯, 张强, 潘晓飚, 李美, 孟丽君, 徐正进, 徐建龙, 黎志康. 3个中籼稻骨干恢复系对稻瘟病和褐飞虱抗性改良效果的评价. 核农学报, 2013, 27: 1069–1080

Chen K, Zhang Q, Pan X B, Li M, Meng L J, Xu Z J, Xu J L, Li Z K. Evaluation on effect of resistance improvement of rice blast and brown planthopper for three dominant Indica restorer lines. J  Nucl Agric Sci, 2013, 27: 1069–1080 (in Chinese with English abstract)

[22]卢华金, 张宏化, 杨文清, 阮柏苗, 马国华, 周海平. 杂交稻II优845的特征特性及栽培、制种要点. 浙江农业科学, 2007, (2): 169–170

Lu H J, Zhang H H, Yang W Q, Ruan B M, Ma G H, Zhou H P. Characteristics of hybrid rice “II you 845” and key points of its cultural techniques and seed production. Zhejiang Agric Sci, 2007, (2): 169–170 (in Chinese)

[23]马国华, 张宏化, 林舜模, 荀飞琼, 卢华金, 徐秀如, 周海平. 杂交晚稻新组合宜优845的选育与应用. 中国稻米, 2009, 15(4): 30–32

Ma G H, Zhang H H, Lin X M, Xiong F Q, Lu H J, Xu X R, Zhou H P. Breeding and application of Yiyou 845, a hybrid rice combination. China Rice, 2009, 15(4): 30–32 (in Chinese)

[24]卢华金, 张宏化, 阮柏苗, 杨文清, 荀飞琼, 马国华, 徐秀如. 高产杂交晚稻新组合K优117. 杂交水稻, 2002, 17(6): 54–55

Lu H J, Zhang H H, Ruan B M, Yang W Q, Xiong F Q, Ma G H, Xu X R. K you 117, a high-yielding late hybrid rice combination. Hybrid Rice, 2002, 17(6): 54–55 (in Chinese)

[25]Zhang Q, Wang C L, Zhao K J, Zhou Y L, Caslana V C, Zhu X D, Li D Y, Jiang Q X. The effectiveness of advanced rice lines with new resistance gene Xa23 to rice bacterial blight. Rice Genet Newsl, 2001, 18: 71–73

[26]Wang J K, Singh R P, Braun H J, Pfeiffer W H. Investigation the efficiency of the single backcrossing breeding strategy through computer simulation. Theor Appl Genet, 2009, 118: 683-694

[27]潘晓飚, 陈凯, 张强, 黄善军, 谢留杰, 李美, 孟丽君, 徐正进, 徐建龙, 黎志康. 分子标记辅助选育水稻抗白叶枯病和稻瘟病多基因聚合恢复系. 作物学报, 2013, 39: 1582–1593

Pan X B, Chen K, Zhang Q, Huang S J, Xie L J, Li M, Meng L J, Xu Z J, Xu J L, Li Z K. Developing restorer lines pyramiding different resistant genes to blast and bacterial leaf blight by marker-assisted selection in rice. Acta Agron Sin, 2013, 39: 1582–1593 (in Chinese with English abstract)

[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[3] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[4] YANG De-Wei, WANG Xun, ZHENG Xing-Xing, XIANG Xin-Quan, CUI Hai-Tao, LI Sheng-Ping, TANG Ding-Zhong. Functional studies of rice blast resistance related gene OsSAMS1 [J]. Acta Agronomica Sinica, 2022, 48(5): 1119-1128.
[5] MA Hong-Bo, LIU Dong-Tao, FENG Guo-Hua, WANG Jing, ZHU Xue-Cheng, ZHANG Hui-Yun, LIU Jing, LIU Li-Wei, YI Yuan. Application of Fhb1 gene in wheat breeding programs for the Yellow-Huai Rivers valley winter wheat zone of China [J]. Acta Agronomica Sinica, 2022, 48(3): 747-758.
[6] ZHAO Hai-Han, LIAN Wang-Min, ZHAN Xiao-Deng, XU Hai-Ming, ZHANG Ying-Xin, CHENG Shi-Hua, LOU Xiang-Yang, CAO Li-Yong, HONG Yong-Bo. Genetic dissection of the bacterial blight disease resistance in super hybrid rice RILs using genome-wide association study [J]. Acta Agronomica Sinica, 2022, 48(1): 121-137.
[7] CHEN Can, NONG Bao-Xuan, XIA Xiu-Zhong, ZHANG Zong-Qiong, ZENG Yu, FENG Rui, GUO Hui, DENG Guo-Fu, LI Dan-Ting, YANG Xing-Hai. Genome-wide association study of blast resistance loci in the core germplasm of rice landraces from Guangxi [J]. Acta Agronomica Sinica, 2021, 47(6): 1114-1123.
[8] HAN Yu-Zhou, ZHANG Yong, YANG Yang, GU Zheng-Zhong, WU Ke, XIE Quan, KONG Zhong-Xin, JIA Hai-Yan, MA Zheng-Qiang. Effect evaluation of QTL Qph.nau-5B controlling plant height in wheat [J]. Acta Agronomica Sinica, 2021, 47(6): 1188-1196.
[9] HUANG Yi-Wen, DAI Xu-Ran, LIU Hong-Wei, YANG Li, MAI Chun-Yan, YU Li-Qiang, YU Guang-Jun, ZHANG Hong-Jun, LI Hong-Jie, ZHOU Yang. Relationship between the allelic variations at the Ppo-A1 and Ppo-D1 loci and pre-harvest sprouting resistance in wheat [J]. Acta Agronomica Sinica, 2021, 47(11): 2080-2090.
[10] ZHENG Kai-Li, JI Zhi-Yuan, HAO Wei, TANG Yong-Chao, WEI Ye-Na, HU Yun-Gao, ZHAO Kai-Jun, WANG Chun-Lian. Molecular identification of rice bacterial blight susceptible gene Xig1 and creation of disease resistant resources [J]. Acta Agronomica Sinica, 2020, 46(9): 1332-1339.
[11] HU Mao-Long, CHENG Li, GUO Yue, LONG Wei-Hua, GAO Jian-Qin, PU Hui-Ming, ZHANG Jie-Fu, CHEN Song. Development and application of the marker for imidazolinone-resistant gene in Brassica napus [J]. Acta Agronomica Sinica, 2020, 46(10): 1639-1646.
[12] Ping ZHANG,Yi-Mei JIANG,Peng-Hui CAO,Fu-Lin ZHANG,Hong-Ming WU,Meng-Ying CAI,Shi-Jia LIU,Yun-Lu TIAN,Ling JIANG,Jian-Min WAN. Introducing qSS-9 Kas into Ningjing 4 by molecular marker-assisted selection to improve its seed storage ability [J]. Acta Agronomica Sinica, 2019, 45(3): 335-343.
[13] ZHANG An-Ning,LIU Yi,WANG Fei-Ming,XIE Yue-Wen,KONG De-Yan,NIE Yuan-Yuan,ZHANG Fen-Yun,BI Jun-Guo,YU Xin-Qiao,LIU Guo-Lan,LUO Li-Jun. Pyramiding and evaluation of brown planthopper resistance genes in water-saving and drought-resistance restorer line [J]. Acta Agronomica Sinica, 2019, 45(11): 1764-1769.
[14] Xiang-Yi XIAO,Xue-Tao SHI,Hao-Wen SHENG,Jin-Ling LIU,Ying-Hui XIAO. Fine Mapping and Candidate Gene Analysis of Rice Blast Resistance Gene Pi47 [J]. Acta Agronomica Sinica, 2018, 44(7): 977-987.
[15] Hong-Bo SHAN,Jia-Wen SHI,Ying SHI. Development and Validation of Molecular Marker for Protein Content in Tetraploid Potato Tuber [J]. Acta Agronomica Sinica, 2018, 44(7): 1095-1102.
Full text



No Suggested Reading articles found!