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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (12): 1779-1790.doi: 10.3724/SP.J.1006.2015.01779

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Development and Application of a Functional Marker of the Blast Resistance Gene Pi35 in Rice

MA Jian**,MA Xiao-Ding**,ZHAO Zhi-Chao,WANG Shuai,WANG Jiu-Lin,WANG Jie,CHENG Zhi-Jun,LEI Cai-Lin*   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2015-04-09 Revised:2015-07-20 Online:2015-12-12 Published:2015-08-28
  • Contact: 雷财林, E-mail: leicailin@caas.cn ** 同等贡献(Contributed equally to this work) E-mail:jian.ma2018@aliyun.com
  • Supported by:

    This research was supported by the National Natural Science Foundation of China (30871606, 31471758) and the Major Project of China on New Varieties of GMO Cultivation (2014ZX08001).

Abstract:

Rice blast is one of the most destructive diseases, and breeding resistant cultivars is considered to be the most economical and effective strategy to control this disease. The Pi35 gene shows partial resistance to leaf blast and has been used as a broad-spectrum and durable resistance source in rice breeding programs in Japan. However, its distribution is not clear in Chinese rice germplasm and cultivars. For the purpose to facilitate the application of Pi35 in rice breeding programs in China, we compared the coding sequences of Pi35 alleles in multiple resistant and susceptible rice cultivars, found a specific nucleotide 3780T which was only present in the functional resistance allele of Pi35, and further developed a Pi35 functional marker (Pi35-dCAPS). Among 281 rice accessions including 10 Fukei 138-derived japonica cultivars, 67 leading cultivars, and 204 accessions of rice mini-core collection of Chinese germplasm, five Fukei 138-derived cultivars (Kenjiandao 3, Kenjiandao 6, Kendao 8, Suijing 3, and Longjing 34) and two mini-core accessions (japonica cv. Funingzipijingzi and indica cv. Ximaxian) were detected to possess the intact Pi35 gene by using the Pi35-dCAPS marker in combination with the genomic sequencing of Pi35. These results will greatly facilitate the utilization of Pi35 in rice breeding programs by marker-assisted selection.

Key words: Rice, Blast disease, Partial resistance gene, Functional marker, Marker-assisted selection

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