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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (11): 1967-1974.doi: 10.3724/SP.J.1006.2011.01967

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Identification of SSR Markers Linked to Oil Content in Peanut (Arachis hypogaea L.) through RIL Population and Natural Population

HUANG Li,ZHAO Xin-Yan,ZHANG Wen-Hua,FAN Zhi-Ming,REN Xiao-Ping,LIAO Bo-Shou,JIANG Hui-Fang*,CHEN Yu-Ning   

  1. Key Laboratory of Oil Crop Biology, Ministry of Agricultural / Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
  • Received:2011-03-23 Revised:2011-07-15 Online:2011-11-12 Published:2011-09-06
  • Contact: 姜慧芳,E-mail:peanutlab@oilcrops.cn

Abstract: Oil content has been an important quality trait for peanut. However, the progress in genetic improvement of oil content is slow. This is mainly due to the complex genetic basis, the high cost in oil content testing, and difficulty in phenotypic selection because of environmental influence. Therefore, marker assisted selection (MAS) is necessary for oil content improvement in peanut breeding. In the present study, F8 RILs derived from a cross of Yuanza 9102×Zhonghua 5 were selected as materials. Ten RILs with extremely low oil content (about 52.91% on an average), twelve RILs with extremely high oil content (about 58.85% on an average) and the two parental lines were used to screen 631 simple sequence repeat (SSR) primers. Thirteen polymorphic bands of seven SSR primers were able to distinguish the extremely high and low oil content lines. Subsequently, the seven SSR primers were used to genotype the total RIL population. Based on the statistics of the SSR data from 20 lines with low oil content (less than 55%) and 45 lines with high oil content (more than 56%), we identified that a SSR marker, 2A5-250/240, was tightly linked to the oil content trait. The band 2A5-250 appeared almost in all low oil content lines with the match rate of 95.0%, while the band 2A5-240 appeared almost in all high oil content lines with the match rate of 88.9%. Detection of the marker 2A5-250/240 was conducted in 11 high oil (55.93% on an average) and 11 low oil (48.41% on an average) content peanut cultivars. The results showed that the match rates of the band 2A5-250 to low oil content materials and 2A5-240 to high oil content materials was 90.9% and 63.6%, respectively. In addition, the band 2A5-240 appeared in 10 of the 19 wild peanut (Arachis) lines with high oil content (about 58.60%). The results based on the analyses using RIL and natural populations demonstrated that the SSR marker 2A5-250/240 was useful for marker-assisted selection of oil content in peanut.

Key words: Peanut (Arachis hypogaea L.), Oil content, SSR, Marker assisted selection

[1]Liao B-S(廖伯寿), Lei Y(雷永), Wang S-Y(王圣玉), Li D(李栋), Huang J-Q(黄家权), Jiang H-F(姜慧芳), Ren X-P(任小平). Genetic diversity of peanut RILs and enhancement for high oil genotypes. Acta Agron Sin (作物学报), 2008, 34(6): 999-1004 (in Chinese with English abstract)

[2]Zhang L-H(张礼红), Tan X-H(谭兴和), Wang F(王锋), Deng J-H(邓洁红), Pan X-H(潘小红). Review on arachis in foreign. Food Sci Technol (食品科技), 2007, (6): 13-16 (in Chinese with English abstract)

[3]Jiang H-F(姜慧芳), Ren X-P(任小平). Genetic diversity of peanut resource on morphological characters and seed chemical components in China. Chin J Oil Crop Sci (中国油料作物学报), 2006, 28(4): 421-426 (in Chinese with English abstract)

[4]Tai Y P, Clyde T Y. Genetic studies of peanut proteins and oils. J Am Oil Chem Soc, 1975, 52(9): 377-385

[5]Yu S-L(禹山林), Yang Q-L(杨庆利), Pan L-J(潘丽娟), Bo W-N(薄文娜). Genetic analysis for oil content of peanut seeds. J Plant Genet Resour (植物遗传资源学报), 2009, 10(3): 453-456 (in Chinese with English abstract)

[6]Chen S-L(陈四龙), Li Y-R(李玉荣), Cheng Z-S(程增书), Liao B-S(廖伯寿), Lei Y(雷永), Liu J-S(刘吉生). Heterosis and genetic analysis of oil content in peanut using mixed model of major gene and polygene. Sci Agric Sin (中国农业科学), 2009, 42(9): 3048-3057 (in Chinese with English abstract)

[7]Wang J-Y(王金彦), Yang Q-L(杨庆利), Yu S-L(禹山林). Development and application of SSR markers in peanut. Chin J Oil Crop Sci (中国油料作物学报), 2009, 31(3): 401-406 (in Chinese with English abstract)

[8]Zhang S F, Ma C Z, Zhu J C, Wang J P, Wen Y C, Fu T D. Genetic analysis of oil content in Brassica napus L. using mixed model of majorgene and polygene. Acta Genet Sin, 2006, 33: 171-180

[9]Zhao J, Becker H C, Zhang D, Zhang Y, Ecke W. Oil content in a European × Chinese rapeseed population: QTL with additive andepistatic effects and their genotype-environment interactions. Crop Sci, 2005, 45: 51-59

[10]Delourme R, Falentin C, Huteau V, Clouet V, Horvais R, Gandon B, Specel S, Hanneton L, Dheu J E, Deschamps M, Margale E, Vincourt P, Renard M. Genetic control of oil content in oilseed rape (Brassica napus L.). Theor Appl Genet, 2006, 113: 1331-1345

[11]Mahmood T, Rahman M H, Stringam G R, Yeh F, Good A G. Identification of quantitative trait loci (QTL) for oil and protein contents and their relationships with other seed quality traits in Brassica juncea. Theor Appl Genet, 2006, 113:1211-1220

[12]Mansur L M, Orf J, Lark K G. Determ in ing the linkage of quantitative trait loci to RFLP maker using extreme phenotype of recombinant inbreds of soybean(Glycine max L. Merr.). Theor Appl Genet, 1993, 86: 914-918

[13]Wu X-L(吴晓雷), Wang Y-J(王永军), He C-Y(贺超英), Chen S-Y(陈受宜), Gai J-Y(盖钧镒), Wang X-C(王学臣). QTLs mapping of some agronomic traits of doybean. Acta Genet Sin (遗传学报), 2001, 28(10): 947-955 (in Chinese with English abstract)

[14]Hong Y-B(洪彦彬), Lin K-Y(林坤耀), Zhou G-Y(周桂元), Li S-X(李少雄), Li Y(李艳), Liang X-Q(梁炫强). Genetic linkage analysis of SSR markers and the gene for dark purple testa color in peanut (Arachis hypogaea L.). Chin J Oil Crop Sci (中国油料作物学报), 2007, 29(1): 35-38 (in Chinese with English abstract)

[15]Wang H(王辉), Shi Y-M(石延茂), Ren Y(任艳), Li S-L(李双铃), Jiao K(焦坤), Yuan M(袁美), Li H-J(李华军). Development of SSR markers for root-knot nematode resistance in peanut. J Peanut Sci (花生学报), 2008, 37(2): 14-17 (in Chinese with English abstract)

[16]Hong Y-B(洪彦彬), Li S-X(李少雄), Liu H-Y(刘海燕), Zhou G-Y(周桂元), Chen X-P(陈小平), Wen S-J(温世杰), Liang X-Q(梁炫强). Correlation analysis of SSR markers and host resistance to Aspergillus flavus infection in peanut (Arachis hypogaea L.) Mol Plant Breed (分子植物育种), 2009, 7(2): 360-364 (in Chinese with English abstract)

[17]Varshney R K, Bertioli D J, Moretzsohn M C, Vadez V, Krishnamurthy L, Aruna R, Nigam S N, Moss B J, Seetha K, Ravi K, He G, Knapp S J, Hoisington D A. The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L.). Theor Appl Genet, 2009, 118: 729-739

[18]Khedikar Y P, Gowda M V C, Sarvamangala C, Patgar K V, Upadhyaya H D, Varshney R K. A QTL study on late leaf spot and rust revealed one major QTL for molecular breeding for rust resisitance in groundnut (Arachis hypogaea L.). Theor Appl Genet, 2010, 121: 971-984

[19]Xia Y-L(夏友霖), Liao B-S(廖伯寿), Li J-N(李加纳), Lei Y(雷永), Jiang H-F(姜慧芳), Cui F-H(崔富华), Zeng X-P(曾孝平). Identification of AFLP markers linked to resistance to late leaf spot in peanut (Arachis hypogaea L.). Chin J Oil Crop Sci (中国油料作物学报), 2007, 29(3): 318-321 (in Chinese with English abstract)

[20]Hou H-M(侯慧敏), Liao B-S(廖伯寿), Lei Y(雷永), Ren X-P(任小平), Wang S-Y(王圣玉), Li D(李栋), Jiang H-F(姜慧芳), Huang J-Q(黄家权), Chen B-Y(陈本银). Identification of AFLP markers for resistance to peanut rust. Chin J Oil Crop Sci (中国油料作物学报), 2007, 29(2): 89-92 (in Chinese with English abstract)

[21]Lei Y(雷永), Liao B-S(廖伯寿), Wang S-Y(王圣玉), Li D(李栋), Jiang H-F(姜慧芳). Identification of AFLP markers for resistance to seed infection by Aspergillus flavus in peanut (Arachis hypogaea L.). Acta Agron Sin (作物学报), 2005, 31(10): 1349-1353 (in Chinese with English abstract)

[22]Liao B-S(廖伯寿), Lei Y(雷永), Li D(李栋), Wang S-Y(王圣玉), Huang J-Q(黄家权), Ren X-P(任小平), Jiang H-F(姜慧芳), Yan L-Y(晏立英). Novel high oil germplasm with resistance to Aspergillus flavus and bacterial wilt developed from recombinant inbred lines. Acta Agron Sin (作物学报), 2010, 36(8): 1296-1301 (in Chinese with English abstract)

[23]Hopkins M S, Casa A M, Wang T, Mitchell S E, Dean R E, Kochert G D, Kresovich S. Discovery and characterization of polymorphic simple sequence repeats (SSR) in peanut. Crop Sci, 1999, 39: 1243-1247

[24]Palmieri D A, Hoshino A A, Bravo J P, Lopes C R, Gimenes M A. Isolation and characterization of microsatellite loci from the forage species Arachis Pintoi (Genus Arachis). Mol Ecol Notes, 2002, 2: 551-553

[25]He G, Meng R, Newman M, Gao G, Pittman R N, Prakash C S. Microsatellites as DNA markers in cultivated peanut (A. hypogaea L.). BMC Plant Biol, 2003, 3: 3

[26]Ferguson M E, Burow M D, Schulze S R, Bramel P J, Paterson A H, Kresovich S, Mitchell S. Microsatellite identification and characterization in peanut (A. hypogaea L.). Theor Appl Genet, 2004, 108: 1064-1070

[27]Moretzsohn M C, Hopkins M S, Mitchell S E, Kresovich S, Valls J F, Ferreira M E. Genetic diversity of peanut (Arachis hypogaea L.) and its wild relatives based on the analysis of hypervariable regions of the genome. BMC Plant Biol, 2004, 4: 11

[28]Moretzsohn M C, Leoi L, Proite K, Guimaras P M, Leal-Bertioli S C M, Gimenes M A, Martins W S, Valls J F M, Grattapaglia D, Bertioli D J. A microsatellite-based, gene-rich linkage map for the AA genome of Arachis (Fabaceae). Theor Appl Genet, 2005, 111: 1060-1071

[29]Palmieri D A, Bechara M D, Curi R A, Gimenes M A, Lopes C R. Novel polymorphic microsatellite markers in section Caulorrhizae (Arachis, Fabaceae). Mol Ecol Notes, 2005, 5: 77-79

[30]Chen B-Y(陈本银), Jiang H-F(姜慧芳), Liao B-S(廖伯寿), Ren X-P(任小平). Phylogenetic relationship among the species in genus Arachis through SSR. J Plant Genet Resour (植物遗传资源学报), 2007, 8(2): 140-144 (in Chinese with English abstract)

[31]Burow M D, Simpson C E, Starr J L, Paterson A H. Transmission genetics of chromatin from a synthetic amphidiploid to cultivated peanut (Arachis hypogaea L.): broadening the gene pool of a monophyletic polyploid species. Genetics, 2001, 159: 823-837

[32]Kantety R V, La Rota M, Matthews D E. Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat. Plant Mol Biol, 2002, 48: 501-510

[33]Upadhyaya H D, Nigam S N. Detection of epistasis for protein and oil contents and oil quality parameters in peanut. Crop Sci, 1999, 39: 115-118

[34]Wynne J C, Coffelt T A. Genetics of Arachis hypogaea L. In: Pattee H E, Young C T, eds. Peanut Science and Technology. Yoakum, TX: American Peanut Research and Education Society, 1982. pp 50-94

[35]Liu E-S(刘恩生). Diallel analysis for yield protein and oil content of peanut. Acta Agric Boreali-Sin (华北农学报), 1987, 2(3): 18-26 (in Chinese with English abstract)
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