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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 814-819.doi: 10.3724/SP.J.1006.2012.00814

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Effect of Successive Backcrossing on Eliminating Somaclonal Variation Caused by Agrobacterium-Mediated Transformation in Rice

YANG Zhou1,2,CHEN Hao1,TANG Wei3,LIN Yong-Jun1,*   

  1. 1 National Key Laboratory of Crop Genetic Improvement / National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan
    430070, China; 2 Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China; 3 Biochemistry Department, Hubei
    University of Medicine, Shiyan 442000, China
  • Received:2011-08-29 Revised:2012-01-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 林拥军, E-mail: yongjunlin@mail.hzau.edu.cn

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Abstract: Somaclonal variation caused by Agrobacterium-mediated transformation influences some agronomic traits of transgenic plants. As a result, a large number of T0 regenerated plants are required in the development of transgenic crop. Here, we successively backcrossed transgenic rice (Oryza sativa L.) to progenitor variety and evaluated agronomic performance of progeny, with the objective of eliminating somaclonal variation and recovering agronomic traits of transgenic parent. Three transgenic lines possessing different insect-resistance genes from Bacillus thuringiensis (Bt) were used as donor parents. They were backcrossed successively to the progenitor variety, and the individuals of each BCnF1 generation were then selfed twice, with selection for insect-resistance. BCnF3 homozygous lines, which were selected by a germination assay, caused an insect mortality of 100% in laboratory bioassay. In the field evaluation, these lines showed obviously higher yield than the donor parents and no significant differences from the progenitor variety in most agronomic traits. These results indicate that successive backcrossing can to a large extent recover agronomic traits of transgenic rice and reduce the workload required in transgenic breeding program.

Key words: Successive backcross, Somaclonal variation, Agrobacterium-mediated transformation, Transgenic rice, Insect-resistance, Agronomic trait

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