Rice (Oryza sativa L.),psf mutant,Ultra-structure,Photosynthesis rate,Chlorophyll fluorescence,"/> Photosynthesis and Chloroplast Ultra-structure Characteristics of Flag Leaves for a Premature Senescence Rice Mutant
Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 871-879.doi: 10.3724/SP.J.1006.2012.00871


Photosynthesis and Chloroplast Ultra-structure Characteristics of Flag Leaves for a Premature Senescence Rice Mutant

WANG Fu-Biao1,HUANG Fu-Deng2,CHENG Fang-Min1,*,LI Zhao-Wei1,HU Dong-Wei1,PAN Gang1,*,MAO Yu-Chan1   

  1. 1 College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; 2 Zhejiang Academy of Agricultural Sciences, Hangzhou 310031, China
  • Received:2011-10-20 Revised:2011-12-19 Online:2012-05-12 Published:2012-02-13
  • Contact: 程方民, E-mail: chengfm@zju.edu.cn; 潘刚, E-mail: pangang12@126.com E-mail:wfb19872856@163.com

Abstract: The influences of leaf premature senescence at the filling stage on photosynthesis rate, chlorophyll fluorescence and chloroplast ultra-structure were investigated using Zhehui 7954 (an excellent indica restorer line) and its leaf senescence mutant (psf). The results indicated that the psf mutant had significantly negative influence on the numbers of filling grains per panicle, seed-setting rate, 1000-grain weight and yield per plant, especially on the numbers of filling grains per panicle and seed-setting rate. Compared with CK, psf also exhibited dramatically lower chlorophyll content, the ratio of chlorophyll a/b, photosynthetic rate (Pn) and chlorophyll fluorescence parameters Fv/Fo and Fv/Fm at filling stage, which were further lowered in the process of maturity. No visual changesin the ultra-structure of mesophyll cells and chloroplasts were observed for psf, showing its intact thylakoid, plastoglobuli size and shape. However, abnormal shape and stacking parallel distribution of chloroplasts, near the cell wall, the degraded grana, the plastoglobuli residue and the destroyed chloroplast membrane could be detected in psf mesophyll cells, in which the appearance of stacking and spherical chloroplasts was responsible for the dropped Pn in psf leaf prior to the degradation and disintegration of thylakoid and grana membrane, thereby caused the decrease of Fv/Fo and Fv/Fm.

Key words: Rice (Oryza sativa L.)')">

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