Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (07): 1303-1308.doi: 10.3724/SP.J.1006.2013.01303

• RESEARCH NOTES • Previous Articles     Next Articles

Effects of Soil Arsenic on Soybean Main Traits and Chlorophyll Content at Different Growing Stage

LI Hai-Bo1,YANG Lan-Fang1,*,LI Ya-Dong2   

  1. 1 School of Resources and Environmental Science, Hubei University, Wuhan 430062, China; 2 School of Life Science, Hubei University, Wuhan 430062, China
  • Received:2012-10-09 Revised:2013-03-11 Online:2013-07-12 Published:2013-04-23
  • Contact: 杨兰芳, E-mail: lfyang@hubu.edu.cn, Tel: 18971612858

Abstract:

Arsenic is ubiquitous in environment and has high toxicity to animal and plant. Investigating the effects of arsenic on plant growth is important for the recognition of the toxic mechanism of arsenic to plant and the food safety. To understand the relationship between soil arsenic pollution and plant growth, we conducted a soil pot experiment using a soybean variety with adding different amounts of arsenic into soil, in which the soybean growth condition was observed and the plant height, chlorophyll content at podding and grain filling stages and the biomass after harvest were determined. The results showed that visible symptom of arsenic toxicity to soybean was dwarfing of plants, dark green and crimpled leaves, retarded maturity when soil arsenic addition was up to 50 mg kg–1. The soybean plant height decreased and the soybean biomass significantly reduced when high soil arsenic contents. The soybean plant height, soybean stem biomass, aerial part biomass, grain yield and the total soybean biomass decreased by 45.0%, 36.6%, 44.6%, 56.1%, and 43.4%, respectively, in the treatments of 100 mg kg–1 of arsenic. High soil arsenic amounts increased the biomass ratios of roots to aerial parts and stem grains to aerial parts, but decreased the ratios of grains to aerial parts, grains to stems and grains to total biomass. Soil arsenic amounts had no significant effects on chlorophyll content in soybean leaf but decreased the ratio of Chl a to Chl b at podding stage. At grain filling stage, 50 and 100 mg kg-1 arsenic treatments increased 120.4% and 96.1% of Chl a, 112.2% and 91.5% of Chl b, 117.8% and 94.5% of total chlorophyll and 104.4% and 83.7% of carotenoid content, respectively. High soil arsenic amounts reduced the ratio of chlorophyll content at podding stage to that at grain filling stage significantly. In conclusion, high soil arsenic amounts are toxic to soybean growth, affect the allocation of soybean biomass, and decrease the biomass of aerial parts and grains yield. The important reason of the retardation of soybean maturity is the constitutional alteration of chlorophyll in soybean leaf and the relative increment of chlorophyll content in soybean leaves during later growing stage by high soil arsenic amounts.

Key words: Soil arsenic, Soybean (Glycine max), Plant height, Biomass, Chlorophyll

[1]Tripathi R D, Srivastava S, Mishra S, Singh N, Tuli F, Gupta D K, Maathuis F J M. Arsenic hazards: strategies for tolerance and remediation by plants. Trends Biotechnol, 2007, 25: 158–165



[2]Mateos-Naranjo E, Andrades-Moreno L, Redondo-Gómez S. Tolerance to and accumulation of arsenic in the cordgrass Spartina densiflora Brongn. Bioresource Technol, 2012, 104: 187–194



[3]Pillai A, Sunita G, Gupta V K. A new system for the spectrophotometric determination of arsenic in environmental and biological samples. Anal Chimica Acta, 2000, 408: 111–115



[4]Mandal B K, Suzuki K T. Arsenic around the world: a review. Talanta, 2002, 58: 201–235



[5]Brammer H, Ravenscroft P. Arsenic in groundwater: a threat to sustainable agriculture in South and Southeast Asia. Environ Internatl, 2009, 35: 647–654



[6]Hu S-Y(胡省英), Ran W-Y(冉伟彦). Ecological effects of arsenic in soil environment. Geophys Geochem Explorat (物化与物探), 2006, 30(1): 83–91(in Chinese with English abstract)



[7]Chang S-M(常思敏), Ma X-M(马新明), Jiang Y-Y(蒋媛媛), He D-X(贺德先), Zhang G-L(张贵龙). Research progress on arsenic contamination in soils and arsenic toxicity in crops. J Henan Agric Univ (河南农业大学学报), 2005, 39(2): 161–167 (in Chinese with English abstract)



[8]Garg N, Singla P. Arsenic toxicity in crop plants: physiological effects and tolerance mechanisms. Environ Chem Lett, 2011, 9: 303–321



[9]Talano M A, Cejas R B, González P S, Agostini E. Arsenic effect on the crop symbiosis Bradythizobium-soybean. Plant Physiol Biochem, 2013, 63: 8–14



[10]Zou Q(邹琦). Experimental Guidebook of Plant Physiology (植物生理实验指导书). Beijing: China Agriculture Press, 2000. pp 72–73 (in Chinese)



[11]Lao J-C(劳家柽). Manual of Soil Agro-Chemical Analysis (土壤农化分析手册). Beijing: China Agriculture Press, 1988. pp 229–354 (in Chinese)



[12]Cao H, Jiang Y, Chen J, Zhang H, Huang W, Li L, Zhang W. Arsenic accumulation in Scutellaria baicalensis Georgi and its effects on plant growth and pharmaceutical components. J Hazardous Materials, 2009, 171: 508–513



[13]Shaibur M R, Kawai S. Effect of arsenic on visible symptom and arsenic concentration in hydroponic Japanese mustard spinach. Environ Exp Bot, 2009, 67: 65–70



[14]Chen T-B(陈同斌), Liu G-L(刘更令). Effect of arsenic on rice (Oryza sativa L) growth and development and its mechanism. Sci Agric Sin (中国农业科学), 1993, 26(6): 50–58 (in Chinese with English abstract)



[15]Liu Q-J(刘全吉), Zheng C-M(郑床木), Tan Q-L(谭启玲), Sun X-C(孙学成), Hu C-X(胡承孝). Effects of high arsenic pollution in soil on growth of winter wheat (Triticum aestivum L.) and rape (Brassica napus). Acta Agric Zhejiangensis (浙江农业学报), 2011, 23(5): 967–971(in Chinese with English abstract)



[16]Aposhian H V, Zakharyan R A, Avram M D, Sampayo-Reyes A, Wollemberg M L. A view of the enzymology of arsenic metabolism and a mew potential role of hydrogen peroxide in the detoxication of the trivalent arsenic species. Toxicol Appl Pharmacol, 2004, 198: 327–335



[17]Smith S E, Christophersen H M, Pope S, Smith F A. Arsenic uptake and toxicity in plants: integrating mycorrhizal influences. Plant Soil, 2010, 327: 1–21



[18]Päivöke A E, Simola L K. Arsenate toxicity to Pisum sativum: mineral nutrients, chlorophyll content, and phytase activity. Ecotoxicol Environ Safety, 2011, 49: 111–121



[19]Liu Q-J(刘全吉), Sun X-C(孙学成), Hu C-X(胡承孝), Tan Q-L(谭启玲). Growth and photosynthesis characteristics of wheat (Triticum aestivum L.) under arsenic stress condition. Acta Ecol Sin (生态学报), 2009, 29(2): 854–859 (in Chinese with English abstract)



[20]Rahman M A, Hasegawa H, Rahman M M, Ialam M N, Miah M A M, Tasmen A. Effect of arsenic on photosynthesis, growth and yield of five widely cultivated rice (Oryza sativa L) varies in Bangladesh. Chemosphere, 2007, 67: 1072–1079



[21]Miteva E, Hristova D, Nenova V, Maneva S. Arsenic as a factor affecting virus infection in tomato plants: changes in plant growth, peroxidase activity and chloroplase pigments. Sci Hort, 2005, 105: 343–358



[22]Stoeva N, Berova M, Zlatev Z. Physiological response of maize to arsenic contamination. Biol Plant, 2003, 47: 449–452



[23]Xu Z-S(徐竹生), Liu D-H(刘道宏). Study of the senescence of rice leaves. J Huazhong Agric Univ (华中农业大学学报), 1986, 5(1): 33–39 (in Chinese with English abstract)



[24]Li J R, Yu K, Wei J R, Ma Q, Wang B Q, Yu D. Gibberellin retards chlorophyll degradation during senescence of Paris polyphylla. Biol Plant, 2010, 54: 395–399



[25]Guan J-Y(关锦毅), Hao Z-B(郝再彬), Zhang D(张达), Wang X-L(王秀丽). A review on the extraction, detection and biological function of chlorophyll. J Northeast Agric Univ (东北农业大学学报), 2009, 40(12): 130–134 (in Chinese with English abstract)
[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] WANG Wang-Nian, GE Jun-Zhu, YANG Hai-Chang, YIN Fa-Ting, HUANG Tai-Li, KUAI Jie, WANG Jing, WANG Bo, ZHOU Guang-Sheng, FU Ting-Dong. Adaptation of feed crops to saline-alkali soil stress and effect of improving saline-alkali soil [J]. Acta Agronomica Sinica, 2022, 48(6): 1451-1462.
[3] YU Chun-Miao, ZHANG Yong, WANG Hao-Rang, YANG Xing-Yong, DONG Quan-Zhong, XUE Hong, ZHANG Ming-Ming, LI Wei-Wei, WANG Lei, HU Kai-Feng, GU Yong-Zhe, QIU Li-Juan. Construction of a high density genetic map between cultivated and semi-wild soybeans and identification of QTLs for plant height [J]. Acta Agronomica Sinica, 2022, 48(5): 1091-1102.
[4] WANG Ze, ZHOU Qin-Yang, LIU Cong, MU Yue, GUO Wei, DING Yan-Feng, NINOMIYA Seishi. Estimation and evaluation of paddy rice canopy characteristics based on images from UAV and ground camera [J]. Acta Agronomica Sinica, 2022, 48(5): 1248-1261.
[5] FU Mei-Yu, XIONG Hong-Chun, ZHOU Chun-Yun, GUO Hui-Jun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, DING Yu-Ping, XU Yan-Hao, LIU Lu-Xiang. Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene [J]. Acta Agronomica Sinica, 2022, 48(3): 580-589.
[6] ZHANG Jia-Kang, LI Fei, SHI Shu-De, YANG Hai-Bo. Construction and application of the critical nitrogen concentration dilution model of sugar beet in Inner Mongolia, China [J]. Acta Agronomica Sinica, 2022, 48(2): 488-496.
[7] WANG Ying, GAO Fang, LIU Zhao-Xin, ZHAO Ji-Hao, LAI Hua-Jiang, PAN Xiao-Yi, BI Chen, LI Xiang-Dong, YANG Dong-Qing. Identification of gene co-expression modules of peanut main stem growth by WGCNA [J]. Acta Agronomica Sinica, 2021, 47(9): 1639-1653.
[8] ZHANG Jian, XIE Tian-Jin, WEI Xiao-Nan, WANG Zong-Kai, LIU Chong-Tao, ZHOU Guang-Sheng, WANG Bo. Estimation of feed rapeseed biomass based on multi-angle oblique imaging technique of unmanned aerial vehicle [J]. Acta Agronomica Sinica, 2021, 47(9): 1816-1823.
[9] LI Jing, WANG Hong-Zhang, LIU Peng, ZHANG Ji-Wang, ZHAO Bin, REN Bai-Zhao. Differences in photosynthetic performance of leaves at post-flowering stage in different cultivation modes of summer maize (Zea mays L.) [J]. Acta Agronomica Sinica, 2021, 47(7): 1351-1359.
[10] HAN Yu-Zhou, ZHANG Yong, YANG Yang, GU Zheng-Zhong, WU Ke, XIE Quan, KONG Zhong-Xin, JIA Hai-Yan, MA Zheng-Qiang. Effect evaluation of QTL Qph.nau-5B controlling plant height in wheat [J]. Acta Agronomica Sinica, 2021, 47(6): 1188-1196.
[11] SHEN Wen-Qiang, ZHAO Bing-Bing, YU Guo-Ling, LI Feng-Fei, ZHU Xiao-Yan, MA Fu-Ying, LI Yun-Feng, HE Guang-Hua, ZHAO Fang-Ming. Identification of an excellent rice chromosome segment substitution line Z746 and QTL mapping and verification of important agronomic traits [J]. Acta Agronomica Sinica, 2021, 47(3): 451-461.
[12] WEI Huan-He, ZHANG Xu-Bin, GE Jia-Lin, MENG Tian-Yao, LU Yu, LI Xin-Yue, TAO Yuan, DING En-Hao, CHEN Ying-Long, DAI Qi-Gen. Dynamics in above-ground biomass accumulation after transplanting and its characteristic analysis in Yongyou japonica/indica hybrids [J]. Acta Agronomica Sinica, 2021, 47(3): 546-555.
[13] JING Xia, ZOU Qin, BAI Zong-Fan, HUANG Wen-Jiang. Research progress of crop diseases monitoring based on reflectance and chlorophyll fluorescence data [J]. Acta Agronomica Sinica, 2021, 47(11): 2067-2079.
[14] FU Hong-Yu, CUI Guo-Xian, LI Xu-Meng, SHE Wei, CUI Dan-Dan, ZHAO Liang, SU Xiao-Hui, WANG Ji-Long, CAO Xiao-Lan, LIU Jie-Yi, LIU Wan-Hui, WANG Xin-Hui. Estimation of ramie yield based on UAV (Unmanned Aerial Vehicle) remote sensing images [J]. Acta Agronomica Sinica, 2020, 46(9): 1448-1455.
[15] BAI Zong-Fan,JING Xia,ZHANG Teng,DONG Ying-Ying. Canopy SIF synergize with total spectral reflectance optimized by the MDBPSO algorithm to monitor wheat stripe rust [J]. Acta Agronomica Sinica, 2020, 46(8): 1248-1257.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!