Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (01): 168-173.doi: 10.3724/SP.J.1006.2015.00168

• RESEARCH NOTES • Previous Articles    

Stability of Six Soybean Isoflavones Solution

LIANG Xiao-Fang,WANG Bu-Jun   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences / Laboratory of Quality Safety Risk Assessment for Cereal Products (Beijing), 100081, China
  • Received:2014-06-30 Revised:2014-09-30 Online:2015-01-12 Published:2014-11-11
  • Contact: 王步军, E-mail: wangbujun@caas.cn E-mail:liangxiaofanglxf@163.com

Abstract: Six chromatographic grade soybean isoflavones solutions daidzin, glycitin, genistin, daidzein, glycitein, and genistein were prepared with a preparative high performance liquid chromatography (Shimadzu LC-20AP). The solution stabilities were monitored in changes of concentrations and purities during a one-month period under temperatures of –20°C, 4°C, 25°C, and 60°C, respectively, and during a six-month period under a temperature of 4°C. Variance analysis of the one-month period experimental results indicated that the concentrations of five soybean isoflavones solutions showed no obvious change under 4°C and 25°C except for genistin solution. No obvious changes in purities for all soybean isoflavones solutions under –20°C, 4°C, and 25°C were observed. Temperatures of 4°C and 25°C were suggested for short-term storage of soybean isoflavones solutions. The t-test of the six-month period experimental results indicated that the concentrations of six chromatographic grade soybean isoflavones solutions increased significantly, but the purities of those did not change after 0, 1, 2, 3, 4, 6, 8, 12, 16, and 24 weeks preservation at 4°C. Leakage of methanol from containers of isoflavones solutions was considered to be the course of concentration increment. This research provided technical helps for the production of the soybean isoflavones standard solutions.

Key words: Soybean isoflavones, Solution purity, Solution concentration

[1]王兆梅, 李琳, 郭祀远, 郑必胜, 黎海彬. 大豆异黄酮结构及其活性分析. 天然产物研究与开发, 2002, 14(3): 70–74

Wang Z M, Li L, Guo S Y, Zheng B S, Li H B. Analysis for the structure and activity of soybean isoflavones. Nat Prod Res Dev, 2001, 14(3): 70–74 (in Chinese with English abstract)

[2]Barnes S. Effect of genistein on in vitro and in vivo models of cancer. J Nutr, 1995, 125: 777–783

[3]He F J, Chen J Q. Consumption of soybean, soy foods, soy isoflavones and breast cancer incidence: Differences between Chinese women and women in Western countries and possible mechanisms. Food Sci Human Wellness, 2013, 2: 146–161

[4]杨学东, 邓志成, 王晶, 丁明玉. 反相高效液相色谱法制备纯化大豆异黄酮糖苷. 色谱, 2006, 24: 363–366

Yang X D, Deng Z C, Wang J, Ding M Y. Preparation of soybean isoflavone glucosides by reversed-phase high performance liquid chromatography. Chin J Chromatogr, 2006, 24: 363–366 (in Chinese with English abstract)

[5]Eisen B, Ungar Y, Shimoni E. Stability of Isoflavones in soy milk stored at elevated and ambient temperatures. J Agric Food Chem, 2003, 51: 2212–2215

[6]Ungar Y, Osundahunsi O F, Shimoni E. Thermal stability of genistein and daidzein and its effect on their antioxidant activity. J Agric Food Chem, 2003, 51: 4394–4399

[7]汪海波, 刘大川, 佘珠花, 汪海婴, 大豆异黄酮类物质的提取、抗氧化性及稳定性研究. 食品科学, 2004, 25(1): 111–114

Wang H B, Liu D C, She Z H, Wang H Y. The study on extracting antioxidative activity and stability of soy isoflavone. Food Sci, 2004, 25(1): 111–114 (in Chinese with English abstract)

[8]应向东, 李敏珍. 硫化锌胶体标准溶液稳定性研究. 化学分析计量, 2012, 21(6): 76–78

Ying X D, Li M Z. Stability of Zinc sulfide colloid standard solution. Chem Anal Meter, 2012, 21(6): 76–78 (in Chinese with English abstract)

[9]孟凡敏, 阚莹. 标准物质稳定性不确定度的评估. 计量学报, 2010, 31: 112–114

Meng F M, Kan Y. Evaluation of the uncertainty contribution of instability for reference material. Acta Metrol Sin, 2010, 31: 112–114(In Chinese with English abstract)

[10]全国标准物质管理委员会. 标准物质定值原则和统计学原理. 北京: 中国质检出版社, 2011. p 79

National Administrative Committee for CMR’s. General and Statistical Principles for Characterization of Reference Materials. Beijing: China Quality Press, 2011. p 79

[11]梁晓芳, 王步军. 应用制备高效液相色谱同时制备6种大豆异黄酮单体的研究方法. 分析测试学报, 2014, 33: 63–67

Liang X F, Wang B J. Simultaneous preparation of six soybean isoflavones monomers by preparative HPLC. J Instrumental Anal, 2014, 33: 63–67 (in Chinese with English abstract)

[12]ISO Guide 35. Certification of Reference Materials—General and Statistical Principles, 2006

[13]李中皓, 唐纲岭, 边照阳, 冯群芝, 王惠平, 马明, 刘惠民. 烟碱纯度标准物质的研制. 烟草科技, 2013, (9): 50–53

Li Z H, Tang G L, Bian Z Y, Feng Q Z, Wang H P, Ma M, Liu H M. Preparation of nicotine purity reference material. Tob Sci Tech, 2013, (9): 50–53 (in Chinese with English abstract)

[14]马康, 赵敏, 王海峰, 邢金京, 黄挺. 茶碱标准物质的研制与定值. 分析测试学报, 2011, 30: 418–424

Ma K, Zhao M, Wang H F, Xing J J, Huang T. Development and certification of theophylline certified reference material. J Instr Anal, 2011, 30: 418–424 (in Chinese with English abstract)

[15]孟凡敏, 郭敬, 阚莹. 标准物质的稳定性探讨. 计量技术, 2011, (5): 21–23

Meng F M, Guo J, Kan Y. Study on stability of standard materials. Measur Tech, 2011, (5): 21–23 (in Chinese)

[16]杨学东, 王莲, 王文洁, 郭鹤男. 大黄蒽醌类标准物质的制备与鉴定. 计量学报, 2010, 31(5A): 88–92

Yang X D, Wang L, Wang W J, Guo H N. Preparation and identification of anthraquinones certified reference materials in Rhei. Acta Metrol Sin, 2010, 31(5A): 88–92 (in Chinese with English abstract)

No related articles found!
Full text



No Suggested Reading articles found!