Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (02): 278-294.doi: 10.3724/SP.J.1006.2016.00278


Microstructure of Glandular Trichomes on Leaf Surface of Sesame and Changes of Trichome Secretions under Drought Condition

SU Shi1,LI Rui-Hang1,LANG Dan-Ying1,ZHANG Ke1,HAO Xiao-Hu1,LIU Yan1,WANG Jun-Wei2,3,XU Hong1,3,*   

  1. 1 College of Life Sciences, Northwest A&F University, Yangling 712100, China; 2 College of Agronomy, Northwest A&F University, Yangling 712100, China;
    3 State Key Laboratory of Crop Stress Biology for Arid Area, Northwest A & F University, Yangling 712100, China
  • Received:2015-04-20 Revised:2015-09-06 Online:2016-02-12 Published:2015-10-08
  • Contact: 徐虹, E-mail: xuh73@163.com, Tel: 13572445613 E-mail:ssuusshhii@126.com


We investigated the microstructure of glandular trichome on sesame leaf surface by scan electron microscope and extracted the trichome secretions using dichloromethane as solvent, and the secretion components under normal and drought conditions were measured by GC/MS to compare the secondary metabolites between sesame varieties. The experiment results indicated that, there were non-glandular hairs, long stalk glandular hairs, short stalk glandular hairs and mucilage hairs on the sesame leaves surface, and the stomata belong to the buttercup type. The secretions of glandular hairs contained a variety of components, of which the main fractions with peak area more than 2% were composed of esters and straight chain saturated alkanes mainly, showing notable differences among four sesame cultivars. Under drought condition, the secreted components changed significantly. Ji9014 with the highest drought resistance among the four cultivars, had ten components, including C36 alkanes and C34 alkanes, which were higher in Ji9014 than in other tested cultivars. We can conclude that, the GC/MS technique is feasible to identify the glandular trichome secretions, and the secretion components are good index to reveal differences between varieties and the effects of drought condition on metabolic pathway in sesame leaves. So, the glandular hairs and secretion components have important application values in sesame germplasm identification and molecular breeding for drought-resistant sesame.

Key words: Sesame leaves, Glandular trichome, Secretions of glandular trichome, Drought condition, Gas chromatography-mass spectrometer

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