Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (02): 278-294.doi: 10.3724/SP.J.1006.2016.00278

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Microstructure of Glandular Trichomes on Leaf Surface of Sesame and Changes of Trichome Secretions under Drought Condition

SU Shi1,LI Rui-Hang1,LANG Dan-Ying1,ZHANG Ke1,HAO Xiao-Hu1,LIU Yan1,WANG Jun-Wei2,3,XU Hong1,3,*   

  1. 1 College of Life Sciences, Northwest A&F University, Yangling 712100, China; 2 College of Agronomy, Northwest A&F University, Yangling 712100, China;
    3 State Key Laboratory of Crop Stress Biology for Arid Area, Northwest A & F University, Yangling 712100, China
  • Received:2015-04-20 Revised:2015-09-06 Online:2016-02-12 Published:2015-10-08
  • Contact: 徐虹, E-mail: xuh73@163.com, Tel: 13572445613 E-mail:ssuusshhii@126.com

Abstract:

We investigated the microstructure of glandular trichome on sesame leaf surface by scan electron microscope and extracted the trichome secretions using dichloromethane as solvent, and the secretion components under normal and drought conditions were measured by GC/MS to compare the secondary metabolites between sesame varieties. The experiment results indicated that, there were non-glandular hairs, long stalk glandular hairs, short stalk glandular hairs and mucilage hairs on the sesame leaves surface, and the stomata belong to the buttercup type. The secretions of glandular hairs contained a variety of components, of which the main fractions with peak area more than 2% were composed of esters and straight chain saturated alkanes mainly, showing notable differences among four sesame cultivars. Under drought condition, the secreted components changed significantly. Ji9014 with the highest drought resistance among the four cultivars, had ten components, including C36 alkanes and C34 alkanes, which were higher in Ji9014 than in other tested cultivars. We can conclude that, the GC/MS technique is feasible to identify the glandular trichome secretions, and the secretion components are good index to reveal differences between varieties and the effects of drought condition on metabolic pathway in sesame leaves. So, the glandular hairs and secretion components have important application values in sesame germplasm identification and molecular breeding for drought-resistant sesame.

Key words: Sesame leaves, Glandular trichome, Secretions of glandular trichome, Drought condition, Gas chromatography-mass spectrometer

[1]张世卿, 张水成. 芝麻素研究进展. 氨基酸和生物资源, 2005, 27(3): 17–21



Zhang S Q, Zhang S C. Development of Study on Sesamin. Amino Acids & Biotic Resour, 2005, 27(3): 17–21 (in Chinese with English abstract)



[2]杨湄, 黄凤洪. 中国芝麻产业现状与存在问题、发展趋势与对策建议. 中国油脂, 2009, 34(1): 7–12



Yang M, Huang F H. Situation, problem, development trend and suggestion of sesame industry in China. China Oils Fats, 2009, 34(1): 7–12 (in Chinese with English abstract)



[3]刘红艳, 赵应忠. 我国芝麻生产·育种现状及展望. 安徽农业科学, 2005, 33: 2475–2476



Liu H Y, Zhao Y Z. The present situation and prospect of sesame breeding and production in China. J Anhui Agric Sci, 2005, 33: 2475–2476 (in Chinese with English abstract)



[4]严寒, 许本波, 赵福永, 何勇, 姚晓鼎, 田志宏. 脱落酸和水杨酸对干旱胁迫下芝麻幼苗生理特性的影响. 干旱地区农业研究, 2008, 26: 163–166



Yan H, Xu B B, Zhao F Y, He Y, Yao X D, Tian Z H. Effects of abscisic acid and salicylic acid on physiological characteristics of sesame seedlings under drought stress. Agric Res Arid Areas, 2008, 26: 163–166 (in Chinese with English abstract)



[5]Hassanzadeh M, Ebadi A, Panahyan-e-Kivi M, Jamaati-e-Somarin S, Saeidi M, Gholipouri A. Investigation of water stress on yield and yield components of sesame(Seesamum indicum L.) in moghan region. Res J Environ Sci, 2009, 3: 239–244



[6]孙建, 饶月亮, 乐美旺, 颜廷献, 颜小文, 周红英. 干旱胁迫对芝麻生长与产量性状的影响及其抗旱性综合评价. 中国油料作物学报, 2010, 32: 525–533



Sun J, Rao Y L, Le M W, Yan T X, Yan X W, Zhou H Y. Effect of drought stress on sesame growth and yield characteristics and comprehensive evaluation of drought tolerance. Chin J Oil Crop Sci, 2010, 32: 525–533 (in Chinese with English abstract)



[7]王晓玲. 水分胁迫对芝麻花期脯氨酸含量的影响. 安徽农学通报, 2007, 13(20): 66–67



Wang X L. Effect of water stress on proline content of sesame florescence. Anhui Agric Sci Bull, 2007, 13(20): 66–67 (in Chinese with English abstract)



[8]王林海, 张艳欣, 危文亮, 张秀荣. 中国芝麻湿害和旱害发生调查与分析. 中国农学通报, 2011, 27(28): 301–306



Wang L H, Zhang Y X, Wei W L, Zhang X R. Investment of water logging and drought effect on the sesame production in China. Chin Agric Sci Bull, 2011, 27(28): 301–306 (in Chinese with English abstract)



[9]陈培, 汪强, 赵莉, 田东风. 水分胁迫对芝麻种子萌发特性的影响. 种子, 2012, 31(4): 83–85



Chen P, Wang Q, Zhao L, Tian D F. Effect of water stress on seed germination characteristics of sesame (Sesamum indicum L.). Seed, 2012, 31(4): 83–85 (in Chinese with English abstract)



[10]黎冬华, 刘文萍, 张艳欣, 王林海, 危文亮, 高媛, 丁霞, 王蕾, 张秀荣. 芝麻耐旱性的鉴定方法及关联分析. 作物学报, 2013, 39: 1425–1433



Li D H, Liu W P, Zhang Y X, Wang L H, Wei W L, Gao Y, Ding X, Wang L, Zhang X R. Identification method of drought tolerance and association mapping for sesame (Sesamum indicum L.). Acta Agron Sin, 2013, 39: 1425–1433 (in Chinese with English abstract)



[11]Abraham S S, Jaleel C A, Zhao C X, Somasundaram R, Azooz M M, Mantivannan P, Panneerselvam R. Regulation of growth and metabolism by paclobutrazol and ABA in Sesamum indicum L. under drought condition Global J Mol Sci, 2008, 3: 57–66



[12]A.saeidi E, Tohidi-Nejad F, Ebrahimi G, Nejad M, Shirzadi M H. Investigation of water stress on yield and some yield components of sesame genotypes (Sesamum indicum L.) in Jiroft region. J Appl Sci Res, 2012, 8: 243–246



[13]Bahrami H, Razmjoo J, Ostadi Jafari A. Effect of drought stress on germination and seedling growth of sesame (Sesamum indicum L.) cultivars. Intl J Agric-Sci, 2012, 2: 423–428



[14]Molaei P, Ebadi A, Namvar A, Bejandi T K. Water relation, solute accumulation and cell membrane injury in sesame (Sesamum indicum L.) cultivars subjected to water stress. Ann Biol Res, 2012, 3: 1833–1838



[15]Wagner G J. Secreting glandular trichomes: more than just hairs. Plant Physiol, 1991, 96: 675–679



[16]Glas J J, Schimmel B C J, Alba J M, Escobar-Bravo R, Schuurink R C, Kant M R. Plant glandular trichomes as targets for breeding or engineering of resistance to herbivores. Int J Mol Sci, 2012, 13: 17077–17103



[17]Tissier A. Glandular trichomes: what comes after expressed sequence tags? Plant J, 2012, 70: 51–68



[18]Yadav R K, Sangwan R S, Sabir F, Srivastava A K, Sangwan N S. Effect of prolonged water stress on specialized secondary metabolites, peltate glandular trichomes, and pathway gene expression in Artemisia annua L.. Plant Physiol Biochem, 2014, 74: 70–83



[19]Sallets A, Beyaert M, Boutry M, Champagne A. Comparative proteomics of short and tall glandular trichomes of Nicotiana tabacum reveals differential metabolic activities. J Proteome Res, 2014,13: 3386–3396



[20]He J, Gao Q, Liao T, Zeng Q. An ecological implication of glandular trichome-sequestered artemisinin: as a sink of biotic/abiotic stress-triggered singlet oxygen. PeerJ PrePrints 3:e1026, 2015, https://dx.doi.org/10.7287/ peerj.preprints.827v1



[21]Fridman E, Wang J H, Iijima Y, Froehlich J E, Gang D R, Ohlrogge J, Pichersky E. Metabolic, genomic, and biochemical analyses of glandular trichomes from the wild tomato species Lycopersicon hirsutum identify a key enzyme in the biosynthesis of methylketones. Plant Cell, 2005, 17: 1252–1267



[22]Croteau R. Biosynthesis and catabolism of monoterpenoids. Chem Rev, 1987, 87: 929–954



[23]McCaskill D, Gershenzon J, Croteau R. Morphology and monoterpene biosynthetic capabilities of secretory cell clusters isolated from glandular trichomes of peppermint (Mentha piperita L.). Planta, 1992, 187: 445–454



[24]Turner G W, Croteau R. Organization of monoterpene biosynthesis in mentha. Immuno cytochemical localizations of geranyl diphosphate synthase, limonene-6-hydroxylase, isopiperitenol dehydrogenase, and pulegone reductase. Plant Physiol, 2004, 136: 4215–4227



[25]Solereder H. Systematic Anatomy of Dicotyledons. Oxford: Clarendon Press, 1908. pp 611–613



[26]李焜章, 芝麻(Sesamom indicum L.)营养器官的解剖构造: II. 叶的解剖构造. 河南师范大学学报, 1987, 53(1): 89–95



Li K Z. The anatomic structure about the vegetative organ of the Sesamum indicum: II. The leaf anatomy structure. J Henan Norm Univ, 1987, 53(1): 89–95 (in Chinese with English abstract)



[27]AbdulRahaman A A, Oladele F A. Types, densities and frequencies of trichomes in some Nigerian vegetable species. Nig J Pure Appl Sci, 2004, 19: 1653–1658



[28]Sullivan C Y, Ross W M. Selecting for drought and heat resistance in grain sorghum. In: Mussell H, Staples R, eds. Stress Physiology in Crop Plants, John Wiley & Sons, New York, NY, USA, 1979. pp 263–281



[29]Clarke J M, Romagosa I, Jana S, Srivastava J P, McCaig T N. Relationship of excised-leaf water loss rate and yield of durum wheat in diverse environments. Can J Plant Sci, 1989, 69: 1075–1081



[30]侯建华, 吕凤山. 玉米苗期抗旱性鉴定研究. 华北农学报, 1995, 10(3): 89–93



Hou J H, Lü F S. A study on drought resistance identification of maize Seedlings. Acta Agric Boreali-Sin, 1995, 10(3): 89–93 (in Chinese with English abstract)



[31]高俊凤. 植物生理学实验指导. 北京: 高等教育出版社, 2006. pp 208–209



Gao J F. Experimental Guide for Plant Physiology. Beijing: Higher Education Press, 2006. pp 208–209 (in Chinese)



[32]张木清, 陈如凯. 作物抗旱分子生理与遗传改良. 北京: 科学出版社, 2005. pp 372–373



Zhang M Q, Chen R K. The Molecular Physiology and Genetic Improvement in Crop Drought Resistance. Beijing: Science Press, 2005. pp 372–373 (in Chinese)



[33]孔光辉. 烤烟烟叶腺毛分泌物提取方法初探. 中国农学通报, 2006, 22(12): 62–65



Kong G H. Study on the extraction method of leaf trichome exudates in flue-cured tobacco. Chin Agric Sci Bull, 2006, 22(12): 62–65 (in Chinese with English abstract)



[34]刘利娥, 宋少华, 刘金盾. 芝麻叶营养成分分析. 食品科技, 2012, 37(2): 45–47



Liu L E, Song S H, Liu J D. Analysis of nutritional components in sesame leaves. Food Sci Technol, 2012, 37(2): 45–47, 51 (in Chinese with English abstract)



[35]李婧婧, 黄俊华, 谢树成. 植物蜡质及其与环境的关系. 生态学报, 2011, 31: 565–574



Li J J, Huang J H, Xie S C. Plant wax and its response to environmental conditions: an overview. Acta Ecol Sin, 2011, 31: 0565–0574 (in Chinese with English abstract)



[36]李魏强, 张正斌, 李景娟. 植物表皮蜡质与抗旱及其分子生物学. 植物生理与分子生物学学报, 2006, 32: 505–512



Li W Q, Zhang Z B, Li J J. Plant epicuticular wax and drought resistance as well as its molecular biology. J Plant Physiol Mol Biol, 2006, 32: 505–512 (in Chinese with English abstract)



[37]Kim K S, Park S H, Jenks M A. Changes in leaf cuticular waxes of sesame (Sesamum indicum L.) plants exposed to water deficit. J Plant Physiol, 2007, 164: 1134–1143



[38]Kim M S, Shim K B, Park S H, Kim K S. Changes in cuticular waxes of developing leaves in sesame (Sesamum indicum L.). J Crop Sci Biotech, 2009, 12: 161–167

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