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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (04): 574-582.doi: 10.3724/SP.J.1006.2016.00574


Characteristics of Photosynthesis and Antioxidation in Rice Photo-oxidation Mutant 812HS

XU Jin-Gang1,LÜ Chuan-Gen2,*,LIU Li1,LÜ Chun-Fang1,MA Jing1,XIA Shi-Jian2,CHEN Guo-Xiang1,GAO Zhi-Ping1,*   

  1. 1 College of Life Sciences, Nanjing Normal University, Nanjing 210023, China; 2 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2015-08-21 Revised:2016-01-11 Online:2016-04-12 Published:2016-01-27
  • Contact: 吕川根, E-mail: lvchuangen@sina.com; 高志萍, E-mail: 08295@njnu.edu.cn E-mail:jingang_0317@163.com
  • Supported by:

    The study was supported by the National Natural Science Foundation of China (31271621), Natural Science Research of Jiangsu Higher Education Institutions (14KJB180011), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Natural Science Foundation of Jiangsu Province (BK20140916), and Jiangsu Collaborative Innovation Center for Modern Crop Production.


To reveal the physiological mechanism of leaf photo-oxidation in a rice mutant 812HS, we compared and analyzed differences of photochemical activity, antioxidative enzyme activities between 812HS and its wild type 812S grown in the field.  Results showed that chlorophyll content, PSII activity, photophosphorylation activity, net photosynthetic rate (Pn) and antioxidative enzymes activities as well as active oxygen contents in 812HS were similar to those in 812S before exposed to high intensity of sunlight. However, all above indexes were affected by a period of high intensity of sunlight. The decreased degree of chlorophyll content, PSII activity, non-cyclic photophosphorylation (NCPS) activity and Pn in 812HS were significantly higher than those in 812S. Besides, high intensity of sunlight led to a lower increase rate of POD and CAT activities in 812HS. Therefore, the contents of O2?, H2O2, and MDA in 812HS became higher than those in 812S. The result implied that the leaf photo-oxidation of mutant 812HS mainly results from higher sensitivity of PSII activities and lower CAT, POD activities under high intensity of sunlight.

Key words: Photo-oxidation, Photochemical activity, Antioxidative enzyme activity

[1] James B, Bertil A. Too much of a good thing: light can be bad for photosynthesis. Trends Biochem Sci, 1992, 17: 61–66

[2] Satoshi K, Takehiro M, Yuhi S, Hiroshi F, Yuji S, Toshio S, Amane M, Katsumi A, Chikahiro M. Cyclic electron flow around PSI functions in the photoinhibited rice leaves. Soil Sci Plant Nutr, 2011, 57: 105–113

[3] Erling Ö, Eva R. On the significance of photoinhibition of photosynthesis in the field and its generality among species. Photosynth Res, 1992, 33: 63–71

[4] Eva R, Gunnar W, Erling Ö. Photoinhibition of photosynthesis in intact willow leaves in response to moderate changes in light and temperature. Physiol Plant, 1991, 83: 390–396

[5] Takahashi S, Badger M R. Photoprotection in plants: a new light on photosystem II damage. Trends Plant Sci, 2011, 16: 53–60

[6] Long S P, Humphries S, Falkowski P G. Photoinhibition of photosynthesis in nature. Annu Rev Plant Physiol Plant Mol Biol, 1994, 45: 633–662

[7] 彭姣凤, 张磊. 光氧化的成因及其削减机制. 生命科学研究, 2006, 4: 83–90

Peng J F, Zhang L. Causes for the formation and reduction mechanisms of photooxidation. Life Sci Res, 2006, 4: 83–90 (in Chinese with English abstract)

[8] 李霞, 严建民, 季本华, 焦德茂. 光氧化和遮荫条件下水稻的光合生理特性的品种差异. 作物学报, 1999, 25: 301–308

Li X, Yan J M, Ji B H, Jiao D M. Varietal difference in photosynthetic characteristics of rice under photooxidation and shading. Acta Agron Sin, 1999, 25: 301–308 (in Chinese with English abstract)

[9] Jin H K, Choon H L. In vivo deleterious effects specific to reactive oxygen species on photosystem I and II after photo-oxidative treatments of rice (Oryza sativa L.) leaves. Plant Sci, 2005, 168: 1115–1125

[10] Ji B, Jiao D. Photoinhibition and photooxidation in leaves of indica and japonica rice under different temperatures and light intensities. Acta Bot Sin, 2001, 43: 714–720

[11] Ye J W, Gong Z Y, Chen C G, Mi H L, Chen G Y. A mutation of OSOTP 51 leads to impairment of photosystem I complex assembly and serious photo-damage in rice. Integr Plant Biol, 2012, 54: 87–98

[12] Zhou Y, Gong Z, Yang Z, Yuan Y, Zhu J, Wang M, Yuan F, Wu S, Wang Z, Yi C, Xu T, Ryom M, Gu M, Liang G. Mutation of the Light-Induced Yellow Leaf 1 Gene, which encodes a geranial reductase, affects chlorophyll biosynthesis and light sensitivity in rice. PLoS One, 2013, 8(9): e75299

[13] 赖东, 夏士健, 吕川根,魏晓东, 刘少奎, 张斌, 廖慧敏, 颜文飞, 宗寿余, 张启军. 水稻光氧化基因LPO1(t)的初步定位. 江苏农业学报, 2012, 28: 1212–1217

Lai D, Xia S J, Lü C G, Wei X D, Liu S K, Zhang B, Liao H M, Yan W F, Zong S Y, Zhang Q J. Mapping a leaf photo-oxidation gene LPO1(t) in rice. Jiangsu Agric Sci, 2012, 28: 1212–1217 (in Chinese with English abstract)

[14] 夏士健, 吕川根. 水稻叶片光氧化研究进展. 杂交水稻, 2012, 27(5): 1–8

Xia S J, Lü C G. Research progress on leaf photooxidation in rice. Hybrid Rice, 2012, 27(5): 1–8 (in Chinese with English abstract)

[15] Arnon D I. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in beta vulgaris. Plant Physiol, 1949, 24: 1–15

[16] Schansker G, Tóth S Z, Strasser R J. Methylviologen and dibromothymoquinone treatments of pea leaves reveal the role of photosystem I in the Chl a fluorescence rise OJIP. Biochim Biophys Acta-Bioenergetics, 2005, 1706: 250–261

[17] Jérémie S, Jean G, Stéphane H, Olivier P, Patrick O, François L, Liliane B. Physiological and biochemical response to photooxidative stress of the fundamental citrus species. Sci Hortic, 2012, 147: 126–135

[18] Ketcham S R, Davenport J W, Warncke K, McCarty R E. Role of the gamma subunit of chloroplast coupling factor 1 in the light-dependent activation of photophosphorylation and ATPase activity by dithiothreitol. Biol Chem, 1984, 259: 7286-7293

[19] Garc??a L C, Hervás A, Nvas C J A, Jiménez D R M, Tena M. Induction of an antioxidant enzyme system and other oxidative stress markers associated with compatible and incompatible interactions between chickpea (Cicer arietinum L.) and Fusarium oxysporum f. spciceris. Physiol Mol Plant Pathol, 2002, 61: 325-337

[20] Mandhania S, Madan S, Sawhney V. Antioxidant defense mechanism under salt stress in wheat seedlings. Biol Plant, 2006, 50: 227-231

[21] Beers R F, Sizer I W. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Biol Chem, 1952, 195: 133-140

[22] Ou L J, Zhang Z Q, Dai X Z, Zou X X. Photooxidation tolerance characters of a new purple pepper. PLoS One, 2013, 8(5): e63593

[23] 王爱国, 罗广华. 植物的超氧物自由基与轻胺反应的定量关系. 植物生理学通讯, 1990, (6): 55–57

Wang A G, Luo G H. Quantitative relation between the reaction of hydroxylamine and superoxide anion radical in plants. Plant Physiol Commun, 1990, (6): 55–57 (in Chinese with English abstract)

[24] 华春, 王仁雷. 杂交水稻及其三系叶片衰老过程中SOD、CAT活性和MDA含量的变化. 西北植物学报, 2003, 23: 406–409

Hua C, Wang R L. Changes of SOD and CAT activities and MDA content during senescence of hybrid rice and three lines leaves. Acta Bot Boreal-Occident Sin, 2003, 23: 406–409 (in Chinese with English abstract)

[25] Takahashi S, Milward S E, Fan D Y, Chow W S, Badger M R. How does cyclic electron flow alleviate photoinhibition in Arabidopsis? Plant Physiol, 2009, 149: 1560–1567

[26] Allen J F. Cyclic, pseudocyclic and noncyclic photophosphorylation: new links in the chain. Trends Plant Sci, 2003, 8: 15–19

[27] 程建峰, 沈允钢. 循环光合磷酸化. 植物生理学通讯, 2008, 44: 844–852

Cheng J F, Shen Y G. Cyclic photophosphoryation. Plant Physiol Commun, 2008, 44: 844–852 (in Chinese with English abstract)

[28] Xu Q, Fu Y, Min H, Cai S, Sha S, Cheng G. Laboratory assessment of uptake and toxicity of lanthanum (La) in the leaves of Hydrocharis dubia (Bl.) backer. Environ Sci Pollut Res, 2012, 19: 3950–3958

[29] Niu X D, Li G R, Kang Z H, Huang J L, Wang G X. Photosynthetic characteristics and antioxidant enzyme system in high chlorophyll rice Gc mutant. Russia Plant Physiol, 2012, 59: 691–695

[30] Fecht C M M, Horst W J. Does apoplastic ascorbic acid enhance manganese tolerance of Vigna unguiculata and Phaseolus vulgaris? Plant Nutr Fert Sci, 2005, 168: 590–599

[31] Aravind P, Prasad M N V. Zinc alleviates cadmium-induced oxidative stress in Ceratophyllum demersum L.: a free floating freshwater macrophyte. Plant Physiol Biochem, 2003, 41: 391–397

[32] Feussner I, Kuhn H, Wasternack C. Lipoxygenase-dependent degradation of storage lipids. Trends Plant Sci, 2001, 6: 268–273

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