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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (02): 171-178.doi: 10.3724/SP.J.1006.2017.00171

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Fertility Alteration in Male Sterile Line BS210 of Wheat

SUN Hui**,ZHANG Feng-Ting**,WANG Yong-Bo,YE Zhi-Jie,QIN Zhi-Lie,BAI Xiu-Cheng,YANG Ji-Fang,GAO Jian-Gang,ZHAO Chang-Ping*   

  1. 赵昌平, E-mail: cp_zhao@vip.sohu.com
  • Received:2016-03-15 Revised:2016-11-03 Online:2017-02-12 Published:2016-11-18
  • Supported by:

    This study was supported by the National Key Technology R&D Development Program of China (2014BAD01B05), the Youth Research Fund of the Beijing Academy of Agriculture and Forestry Sciences (QNJJ201307), the Technology Innovation Program of the Beijing Academy of Agriculture and Forestry Sciences (CXJJ201307), the Beijing Science and Technology (Z141100002314017), and the Special Project for Enhancing Innovation Ability of the Beijing Academy of Agriculture and Forestry Sciences (KJCX20150705).

Abstract:

Two-line hybrid wheat is an innovation in China and its critical technique is the application of thermo-photo-sensitive male sterile line. BS210 is a male sterile wheat line developed by our group. We studied the fertility transition characteristics of BS210 with differentially sowing tests at three ecological locations and contorlled experiments in growth chambers from 2012 to 2014. In the hybrid seed-produing locations, BS210 showed sterility of 95–100% under suitable sowing conditions with the safe sowing dates from September 30th to October 10th in Fuyang of Anhui and September 30th in Lijiang of Yunnan. In the self-reproducting locations, BS210 showed fertility of 70% under late sowing conditions with the safe sowing dates of November 10th in Haidian of Beijing and from September 30th to November 10th in Shunyi of Beijing. The fertility alteration of BS210 was induced mainly by temperature rather than photoperiod. The phase from pollen mother cell formation to mono-nucleate was sensitive to fertility alteration of BS210. The critical temperature of alteration was 10–12°C under the photoperiod of 10.0–12.5 h and 8–10°C under the photoperiod of 14 h. The pollen-staining observation indicated that the sterile pollen grains belonged to stained abortion type under short-day and low-temperature condition and the fertile pollen grains were round in shape and dark blue in color under long-day and high-temperature condition. Defining the fertility transition characteristics of BS210 will provide theoretical guidelines for selecting suitable ecological zone to ensure safe seed production.

Key words: Two-line hybrid wheat, Thermo-photo-sensitive male sterile line, Fertility alteration

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