Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (10): 1499-1506.doi: 10.3724/SP.J.1006.2017.01499

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Genetic Characters of Fragrance in a New Fragrant Rice Variety Yuhui 2103

WANG Chun-Ping1,**,ZHANG Xian-Wei2,**,BAI Wen-Qin1,JIANG Xiao-Ying1,WU Hong1,LIN Qing1,TANG Yong-Qun2,YAO Xiong2,ZHANG Wu-Jun2,TANG Rong-Li,LI Jing-Yong2,*,LEI Kai-Rong1,*   

  1. 1 Biotechnology Research Center, Chongqing  Academy of Agricultural Sciences, Chongqing 401329, China; 2 Chongqing Ratooning Rice Research Center, Chongqing Academy of Agricultural Sciences, Chongqing 402160, China
  • Received:2016-12-05 Revised:2017-04-19 Online:2017-10-12 Published:2017-05-23
  • Contact: 李经勇, E-mail: ljy@cqagri.gov.cn; 雷开荣, E-mail: leikairong@126.com E-mail:wcp49930000@sina.com
  • Supported by:

    This work was supported by Chongqing Agriculture Seed Innovation and Major Research Extension Project (NKY2016AA003), Chongqing Agriculture Basic Research Project (NKY2016AC024), Chongqing Basic and Frontier Research Program (cstc2016jcyjA0091) and Chongqing Basic Research Fund (2014cstc-jbky-00549).

RichHTML

PDF

965

Abstract:

A major component 2-Acetyl-1-pyrroline (2AP) in fragrance is one of the important indices of high-quality rice, however, the biosynthetic pathway of 2AP has not been demonstrated clearly. The betaine aldehyde dehydrogenase 2 (BADH2) is considered to be closely related to 2AP biosynthesis. In this study, three mutations in Badh2 gene were screened from thirty rice varieties and a new fragrant rice variety Yuhui 2103 was discovered. The new variety has no mutation in the coding region of Badh2 gene and the Badh2 allele of Yuhui 2103 can complement the defect of the badh2-E7 allele in Yixiang 1B. From crosses between Yuhui 2103 and non-fragrant varieties the segregating ratios of F2 fragrant to non-fragrant individuals were 9:7, while from crosses between Yuhui 2103 and fragrant varieties the segregating ratios of F2 fragrant to non-fragrant individuals were 7:9, indicating that fragrance of Yuhui 2103 is not controlled by only one gene. Furthermore, expression patterns of genes involved in 2AP biosynthesis were examined by the quantitative real-time PCR (qRT-PCR) in Nipponbare, Yuhui 2103, and Yixiang 1B. There was no significant difference in transcription level of Badh2 in Nipponbare and Yuhui 2103, however, the transcription level of Badh2 inYixiang 1B was unexpectedly high. The expression levels of most of the genes involved in proline and glutamatic acid metabolism were significantly higher in Yixiang 1B than that in Nipponbare and Yuhui 2103. It is proposed that 2AP biosynthesis in Yixiang 1B is both influenced by Badh2 and the genes involved in proline and glutamatic acid metabolism, however, there is no necessary relationship between these genes and the fragrance of Yuhui 2103. The novel genetic characters of Yuhui 2103 may bring new breakthrough to the study of fragrance formation mechanism in rice.

Key words: Fragrant rice, 2-Acetyl-1-pyrroline, Proline, Glutamatic acid, Badh2, Genetic character

[1] Bradbury L M T, Fitzgerald T L, Henry R J, Jin Q S, Waters D L E. The gene for fragrance in rice.Plant Biotechnol J, 2005, 3: 363–370
[2] Bradbury L M T, Gillies S A, Brushett D J, Waters D L E. Inactivation of an aminoaldehyde dehydrogenase is responsible for fragrance in rice. Plant Mol Biol, 2008, 68: 609–616
[3] Chen S H, Wu J, Yang Y, Shi W W, Xu M L. The fgr gene responsible for rice fragrance was restricted within 69 kb. Plant Sci, 2006, 17: 505–514
[4] Chen S H, Yang Y, Shi W W, Ji Q, He F, Zhang Z D, Cheng Z K, Liu X N, Xu M L. Badh2, encoding betaine aldehyde dehydrogenase, inhibits the biosynthesis of 2-acetyl-1-pyrroline, a major component in rice fragrance. Plant Cell, 2008, 20: 1850–1861
[5] Romanczyk L J, Mcclelland C A, Post L S, Aitken W M. Formation of 2-acetyl-l-pyrroline by several Bacillus cereus strains isolated from cocoa fermentation boxes. J Agric Food Chem, 1995, 43: 469–475
[6] Yoshihashi T, Huong N T T, Kabaki N. Quality evaluation of Khao Dawk Mali 105, an aromatic rice variety of northeast Thailand. JIRCAS Work Rep, 2002, 30: 151–160
[7] Yoshihashi T, Huong N T T, Inatomi H. Precursors of 2-acetyl-1-pyrroline, a potent flavor compound of an aromatic rice variety. J Agric Food Chem, 2002, 50: 2001–2004
[8] Trossat C, Rathinasabapathi B, Hanson A D. Transgenically expressed bataine aldehyde dehydrognase efficiently catalyzes oxidation of dimethylsulfoniopropionaldehyde and ω-aminoaldehydes. Plant Physiol, 1997, 113: 1457–1461
[9] Huang T C, Teng C S, Chang J L, Chuang H S, Ho C T, Wu M L. Biosynthetic mechanism of 2-acetyl-1-pyrroline and its relationship with ?1-pyrroline-5-carboxylic acid methylglyoxal in aromatic rice (Oryza sativa L.) callus. J Agric Food Chem, 2008, 56: 7399–7404
[10] Schieberle P. Quantitation of important roast-smelling odorants in popcorn by stable isotope dilution assays and model studies on flavor formation during popping. J Agric Food Chem, 1995, 43: 2442–2448
[11] He Q, Park Y J. Discovery of a novel fragrant allele and development of functional markers for fragrance in rice. Mol Breed, 2015, 35: 217–227
[12] Shi W W, Yang Y, Chen S H, Xu M L. Discovery of a new fragrance allele and the development of functional markers for breeding of fragrant rice varieties. Mol Breed, 2008, 22: 185–192
[13] Shi Y Q, Zhao G C, Xu X L, Li J Y. Discovery of a new fragrance allele and development of functional markers for identifying diverse fragrant genotypes in rice. Mol Breed, 2014, 33: 701–708
[14] Fitzgerald M A, Hamilton N R S, Calingacion M N, Verhoeven H A, Burtado V M. Is there a second fragrance gene in rice？Plant Biotechnol J, 2008, 6: 416–423
[15] Amarawathi Y, Singh R, Singh A K,Singh V P. Mapping of quantitative trait of loci for basmati quality traits in rice (Oriza sativa L.). Mol Breed, 2007, 21: 49–65
[16] Pachauri V, Mishra V, Mishra P, Singh A K, Singh S, Singh R, Singh N K. Identification of candidate genes for rice grain aroma by combining QTL mapping and transcriptome profiling approaches. Cereal Res Commun, 2014, 42: 376–388
[17] Cheng A, Ismail I, Osman M, Hashim. Mapping of quantitative trait loci for aroma, amylose content and cooked grain elongation traits in rice. Plant Omics, 2014, 7: 152–157
[18] Sood B G, Siddiq E A. A rapid technique for scent determination in rice. Indian J Genet Plant Breed, 1978, 38: 268–275
[19] Dhulappanavar C V. Inheritance of scent in rice. Euphytica, 1975, 25: 659–666
[20] Forlani G, Bertazzini M, Zarattini M, Funk D. Functional characterization and expression analysis of rice δ1-pyrroline-5-carboxylate dehydrogenase provide new insight into the regulation of proline and arginnine catabolism. Front Plant Sci, 2015, 6: 591
[21] Pinson S R M. Inheritance of aroma in six rice cultivars. Crop Sci, 1994, 34: 1151–1157
[22] Hinge V R, Patil H B, Nadaf A. Aroma volatile analyses and 2AP characterization at various developmental stages in Basmati and Non-Basmati scented rice (Oryza sativa L.) cultivars. Rice, 2016, 9: 38–50
[23] Lorieux M, Petrov M, Huang N, Guiderdoni E, Ghesquière A. Aroma in rice: genetic analysis of a quantitative trait. Theor Appl Genet, 1996, 93: 1145–1151
[1] ZENG Zi-Jun, ZENG Yu, YAN Lei, CHENG Jin, JIANG Cun-Cang. Effects of boron deficiency/toxicity on the growth and proline metabolism of cotton seedlings [J]. Acta Agronomica Sinica, 2021, 47(8): 1616-1623.
[2] WANG Wu-Bin, TONG Fei, KHAN Mueen-Alam, ZHANG Ya-Xuan, HE Jian-Bo, HAO Xiao-Shuai, XING Guang-Nan, ZHAO Tuan-Jie, GAI Jun-Yi. Detecting QTL system of root hydraulic stress tolerance index at seedling stage in soybean [J]. Acta Agronomica Sinica, 2021, 47(5): 847-859.
[3] WANG Cui-Ping,HUA Xue-Jun,LIN Bin,LIU Ai-Hua. Evolutionary Fate and Expression Pattern of Genes Related to Proline Biosynthesis in Brassica napus [J]. Acta Agron Sin, 2017, 43(10): 1480-1488.
[4] WU Liang-Liang,YAO Lei,MA Rui,ZHU Xi,YANG Jiang-Wei,ZHANG Ning*,SI Huai-Jun. Cloning and Functional Identification of the ATHB12 Gene of HD-Zip IFamily in Potato(Solanum tuberosum L.) [J]. Acta Agron Sin, 2016, 42(08): 1112-1121.
[5] Lü Gao-Qiang,WU Xiang-Yang,WANG Xin-Yu. Cloning and Characterization of a Novel Gene Encoding Proline-Rich Protein in Sesame [J]. Acta Agron Sin, 2015, 41(12): 1810-1818.
[6] ZHOU Jing-Hua, XING Hu-Cheng,JIE Yu-Cheng, ZHONG Yin-Li, ZHU Shou-Jing, JIANG Jie, WANG Liang. Molecular Cloning and Express Analysis of Δ1-Pyrroline-5-Carboxylate Synthetase (P5CS) Gene in Ramie [J]. Acta Agron Sin, 2012, 38(03): 549-555.
[7] CHEN Ji-Bao,ZHAO Li-Ying,MAO Xin-Guo,WANG Shu-Min,JING Rui-Lian. Response of PvP5CS1 Transgenic Arabidopsis Plants to Drought-and Salt-Stress [J]. Acta Agron Sin, 2010, 36(1): 147-153.
[8] CHEN Ji-Bao;JING Rui-Lian;MAO Xin-Guo;CHANG Xiao-Ping;WANG Shu-Min. A Response of PvP5CS2 Gene to Abiotic Stresses in Common Bean [J]. Acta Agron Sin, 2008, 34(07): 1121-1127.
[9] XU Wen-Liang;HUANG Geng-Qing;WANG Xiu-Lan;TAI Fu-Ju;WANG Hong;LI Xue-Bao. Molecular Characterization and Expression Analysis of Two Genes Encoding Hybrid Proline-Rich Proteins in Cotton [J]. Acta Agron Sin, 2007, 33(07): 1146-1153.
[10] WU Cui;SHAO Guo-Jun;MA Lian-Ju;CUI Xin-Fu;CAO Ping;YIN Chang-Bin;XU Xing-Wei;HE Ping;LÜ Wen-Yan. Genetic Research for Gelatinization Temperature (GT) in Japonica Rice [J]. Acta Agron Sin, 2007, 33(06): 1041-1044.
[11] JIAN Ling-Cheng;LU Cun-Fu;LI Ji-Hong;LI Paul H. Increment of Chilling Tolerance and Its Physiological Basis in Chilling-sensitive Corn Sprouts and Tomato Seedlings after Cold-hardening at Optimum Temperatures [J]. Acta Agron Sin, 2005, 31(08): 971-976.
[12] Zong Hui;Liu EE;Guo Zhenfei;Li Mingqi. Effects of LaCl3 and CPZ on Proline Accumulation of Rice Seedling under Drought and Salt Stresses [J]. Acta Agron Sin, 2001, 27(02): 173-177.
[13] Tao Longxing;Wang Xi;Yu Meiyu;Huang Xiaolin. Bio-effects and Mechanism of CM268 on Inducing Rice Male Sterility [J]. Acta Agron Sin, 2001, 27(02): 178-184.
[14] Chen Cuixia Yu Yuanjie Wang Honggang Shen Fafu Liu Fengzhen. RAPD Analysis and Physiologic Studies on Salt-tolerance Variation of Cotton [J]. Acta Agron Sin, 1999, 25(05): 643-646.
[15] Bao Jusong;Yang Chengshu;Xue Jiquan;Hao Yinchuan. Effect of Water Stress during Different Growing Period of Maize on Its Physiological Characteristics [J]. Acta Agron Sin, 1991, 17(04): 261-266.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd