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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (12): 1827-1834.doi: 10.3724/SP.J.1006.2017.01827

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Application of Quantitative Graphical Method Based on Small Angle X-Ray Scattering Spectrum in Crop Starch Study

HE Wei1,FAN Xiao-Xu1,WANG Zhi-Feng2,WEI Cun-Xu1,*   

  1. 1 Jiangsu Key Laboratory of Crop Genetics and Physiology / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China; 2 Testing Center, Yangzhou University, Yangzhou 225009, China
  • Received:2017-01-09 Revised:2017-09-10 Online:2017-12-12 Published:2017-09-28
  • Contact: 韦存虚, E-mail: cxwei@yzu.edu.cn, Tel: 0514-87997217
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31570324) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Abstract:

Small-angle X-ray scattering (SAXS) spectrum can be used to quantify the lamellar structure of starch. However, the spectrum analysis lacks a special data analysis software and needs complicated mathematical equations for it, which seriously restricts the applications of SAXS technology in starch studies. In this study, a simple graphical method was established to quantitatively measure the SAXS spectrum parameters (peak intensity, peak position, peak width at half maximum, and lamellar distance). These parameters can reflect the information of starch lamellar structure. The SAXS spectra of starch with different crystal types, rice starch with different amylose contents, and acid-modified and gelatinized starch were all analyzed using the graphical method, showing that the lamellar structure of starch was related with the plant origin, but had no direct relationship with crystal types. For the starch from the same plant origin, the amylose content was significantly negatively correlated with peak intensity and peak width at half maximum, and positively correlated with lamellar distance of SAXS spectrum. The acid hydrolysis had no effect on lamellar distance of starch, but changed the peak intensity and peak width at half maximum of SAXS spectrum. Heating treatment also had no effect on lamellar distance of starch, but destroyed the crystalline structure, leading to the gradual decrease, even disappearance of peak intensity with increasing heating temperature. This study indicated that the quantitative graphical method is simple, good repeatable, and highly credible, and could be widely used in crop starch studies.

Key words: Crop starch, Small-angle X-ray scattering spectrum, Spectrum parameter, Quantitative graphical method, Lamellar

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