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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (12): 1817-1826.doi: 10.3724/SP.J.1006.2017.01817

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Mitigative Effect of Different Forms of Boron on Aluminum Toxicity of Rape Seedlings and Its FTIR Characteristics

YAN Lei, JIANG Cun-Cang*, Muhammad Riaz, WU Xiu-Wen, LU Xiao-Pei, DU Chen-Qing,WANG Yu-Han   

  1. Institute of Resource and Environmental Sciences, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2017-03-06 Revised:2017-07-19 Online:2017-12-12 Published:2017-08-07
  • Contact: 姜存仓, E-mail: jcc2000@mail.hzau.edu.cn E-mail:421655346@qq.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (41271320).

Abstract:

An experiment was conducted in hydroponics using rape cultivar Cao 221167. Two treatment of B, inorganic borate (BA) and organic boron sorbitol (SB) along with different concentrations of Al3+ (0, 100, 200, and 500 μmol L–1). The effects of different forms of B on activities of antioxidant enzyme of leaves in rape seedlings were studied, and the changes of leaf component were analyzed by Fourier Transform Infrared Spectroscopy (FTIR) technique. Under Al toxicity, the addition of BA or SB could alleviate Al toxicity in plant, and significantly increase plant biomass and root length. The root length increased by 52.15%, 101.45%, 366.70%, and 18.73% in BA treatments and 46.80%, 133.98%, 261.36%, and 10.77% in SB treatments under 0, 100, 200, and 500 μmol L–1 Al3+ toxicity stress, respectively. B treatments increased pigment content and SOD activity and reduced contents of Al, MDA and POD activity. Under the addition of boron (BA or SB), the growth, biomass and pigment content of rape seedlings were higher in 200 μmol L–1 Al3+ treatment than in other treatments. In the 500 μmol L–1 Al3+ treatment, biomass and pigment content of rape seedling were significantly lower than those without Al application. The results from FTIR indicated that the contents of protein and oligosaccharide in leaves of rape seedling significantly increased under Al toxicity, however, significantly decreased when was added boron (BA or SB). In conclusion, the addition of different forms of B (BA or SB) could alleviate Al toxicity and the effect of BA is better than that of SB. The information provided by this study will facilitate alleviating Al toxicity in plant through applying a kind of B fertilizer in agricultural production.

Key words: Rape, Different forms of boron, Aluminum toxicity stress, FTIR, Mitigation effect

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