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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (04): 533-541.doi: 10.3724/SP.J.1006.2018.00533

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Detection of QTLs for Plant Height Related Traits in Brassica napus L. Using DH and Immortalized F2 Population

Ya-Jun HE1(), Dao-Ming WU1, Ying FU1,2, Wei QIAN1,*()   

  1. 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
    2 Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China
  • Received:2017-07-11 Accepted:2017-11-21 Online:2018-01-20 Published:2017-12-01
  • Contact: Wei QIAN E-mail:hyj790124@163.com;qianwei666@hotmail.com
  • Supported by:
    The study was supported by the National Natural Science Foundation of China (31671729), the National Key Research and Development Program of China (2016YFD0100202), and Chongqing Research Program of Basic Research and Frontier Technology (cstc2017jcyjAX0391).

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Abstract:

Plant height is one of the important agronomic traits in rapeseed. In this study, QTLs for plant height and plant height related traits in Brassica napus were evaluated in two years environments by using a doubled haploid (DH) population lines derived from the cross between two rapeseed cultivars, Express and SWU07, and an immortalized F2 population generated by randomly permuted intermating of these DH lines. A total of 41 putative QTLs were identified for plant height and plant height related traits and located on 13 linkage groups. Among them, nine QTLs were identified for plant height, located on A02, A09, C01, C02, and C06, respectively, with explained phenotypic variation ranging from 3.85% to 13.34%, 15 QTLs were identified for inflorescence length, located on A01, A02, A05, A08, A09, C01, C03, and C05, respectively, with explained phenotypic variation ranging from 3.82% to 9.52%, 11 QTLs were identified for the first branch height, located on A01, A03, A09, C01, and C03, respectively, with explained phenotypic variation ranging from 4.01% to 16.54%, four QTLs were identified for branch segment, located on A07, A09, C03, and C04, respectively, with explained phenotypic variation ranging from 4.79% to 8.10%, two QTLs were identified for average internode length, located on A07 and C05, respectively, with explained phenotypic variation ranging from 4.29% to 6.04%. Five of these QTLs were persistently expressed in different year environments or in different populations. These QTLs provide useful information for improving plant height in rapeseed breeding.

Key words: Brassica napus, plant height related traits, QTL

Fig. 1

Parental lines, SWU07 and Express"

Fig. 2

Frequency distribution of plant height related traits from DH and IF2 populations in 2010 and 2011"

Table 1

Analysis of five plant height related traits in the two parents and two populations in 2010 and 2011 (cm)"

年份及性状
Year and trait		 亲本 Parent DH群体 DH population IF2群体 IF2 population
Express SWU7 最大值
Max.		 最小值
Min.		 平均值
Average		 标准差
SD		 最大值
Max.		 最小值
Min.		 平均值
Average		 标准差
SD
2010
株高PH 187.90 171.90 210.25 134.75 174.63 13.59 209.50 167.50 189.33 9.33
第1分枝高BH 64.42 54.21 96.75 0 53.73 12.03 98.25 20.88 70.78 10.33
主花序长MFL 62.45 66.64 95.00 43.25 69.74 9.54 81.38 50.25 66.93 6.74
分枝区段长BSL 61.03 51.05 103.75 7.00 51.44 15.75 89.62 20.12 51.62 9.77
平均节间长AIL 8.14 7.38 16.38 0.27 6.69 2.22 15.24 3.29 8.00 1.45
2011
株高PH 188.20 172.4 211.94 146.38 176.51 12.03 207.00 149.00 183.64 10.28
第1分枝高BH 64.11 54.48 82.19 0 56.17 10.89 84.50 36.25 56.62 8.48
主花序长MFL 62.89 67.24 96.00 40.42 67.08 8.71 94.25 48.88 75.98 7.62
分枝区段长BSL 61.20 50.68 109.31 10.12 53.18 16.62 73.74 28.00 51.04 7.24
平均节间长AIL 8.34 6.90 15.33 1.49 7.34 2.31 9.72 4.75 6.71 0.70

Table 2

Correlation analysis for plant height related traits in 2010 and 2011"

群体
Population		 株高
PH		 第1分枝高
BH		 主花序长
MFL		 分枝区段长
BS		 平均节间长
AIL
DH 相关系数r 0.71173 0.59625 0.62933 0.62424 0.56011
P值 P-value <0.0001 <0.0001 <0.0001 <0.0001 <0.0001
IF2 相关系数r 0.42004 0.27913 0.44481 0.08834 0.09174
P值 P-value <0.0001 <0.0001 <0.0001 0.23700 0.22060

Table 3

Correlation coefficients among plant height-related traits in DH and IF2 populations"

性状
Trait		 株高
PH		 第1分枝高
BH		 主花序长
MFL		 分枝区段长
BS		 平均节间长
AIL
2010
株高PH 0.37004** 0.38887** 0.29524** 0.15032*
第1分枝高BH 0.05025 -0.16880* -0.58786** -0.52719**
主花序长MFL 0.56513** 0.00826 -0.13980 -0.11797
分枝区段长BS 0.46819** -0.70657** -0.12289* 0.78030**
平均节间长AIL 0.40174** -0.66423** -0.03479 0.85850**
2011
株高PH 0.50163** 0.55404** 0.24941** 0.19897**
第1分枝高BH -0.09142 -0.06965 -0.38554** -0.31154**
主花序长MFL 0.42398** 0.00725 -0.18439** 0.08776
分枝区段长BS 0.56407** -0.72415** -0.22219** 0.55513**
平均节间长AIL 0.51891** -0.71135** -0.10067 0.89344**

Fig. 3

Putative QTLs for piant height related traita on different linkage group"

Table 4

Putative QTLs for plant height related traits detected in DH and IF2 populations."

QTL名称
QTL name		 位置
Position		 加性效应
Additive		 贡献率
R2(%)		 LOD值
LOD score		 置信区间
Confidence interval
qPH-A02-1-2010IF2 0.01 -7.4375 5.8060 2.6304 0-4.6
qPH-A02-2011DH 46.01 -2.4401 3.8457 2.6262 45.0-50.3
qPH-A02-2-2010IF2 6.81 -8.4548 8.5134 4.1982 5.9-7.3
qPH-A09-2010DH 55.81 -3.3042 5.6678 3.0804 53.3-59.3
qPH-C01-1-2010DH# 36.01 3.5905 6.8271 4.7230 35.8-36.4
qPH-C01-1-2011DH# 35.01 3.6424 9.0971 6.1127 34.9-36.1
qPH-C01-2010IF2 27.41 2.1962 5.2034 2.9173 25.3-29.9
qPH-C01-2-2010DH# 41.61 5.0102 13.3370 5.1212 40.6-42.9
qPH-C01-2-2011DH# 41.61 4.1570 11.7177 4.7139 40.3-42.9
qPH-C02-2011IF2 20.91 -3.6501 6.3109 3.7199 19.9-22.4
qPH-C06-2010DH 0.01 -2.7798 3.8592 2.7071 0-4.9
qMFL-A01-2010DH 10.31 2.5968 4.6167 3.0365 9.1-13.8
qMFL-A02-1-2011DH# 4.21 -2.6647 4.8134 3.5387 1.2-7.2
qMFL-A02-2011IF2# 7.31 -4.3769 3.8153 2.6593 5.7-7.9
qMFL-A02-2-2011DH 10.91 -2.6231 6.6987 4.0032 9.4-13.0
qMFL-A05-1-2011DH 123.21 1.8066 4.0520 3.0253 118.5-125.2
qMFL-A05-2-2011DH 130.91 1.8008 4.0021 2.7637 125.2-136.9
qMFL-A08-1-2011DH 10.81 -1.9022 4.5131 3.1474 8.5-18.8
qMFL-A08-2-2011DH 21.11 -1.9707 4.9151 3.6317 19.5-23.8
qMFL-A09-1-2010DH 10.51 -2.8631 5.3042 2.5221 3.8-12.5
qMFL-A09-2-2010DH 98.81 -2.5999 4.4557 3.0245 94.7-99.8
qMFL-C01-1-2011DH 26.41 1.9285 4.2338 2.6209 25.6-27.4
qMFL-C01-2010IF2# 29.31 1.6312 5.6672 3.0765 28.8-30.1
qMFL-C01-2011IF2# 29.31 1.9919 6.5915 3.9887 28.7-29.9
qMFL-C01-2-2011DH 31.71 2.7168 9.5163 4.4021 30.7-33.1
qMFL-C03-1-2011DH# 15.31 2.2077 5.3394 3.4259 14.8-19.8
qMFL-C03-2011IF2# 17.61 1.9917 6.5247 4.2625 16.2-18.8
qMFL-C03-2-2011DH 24.81 2.3843 5.5196 3.7784 24.7-30.4
qMFL-C05-2010IF2 6.81 1.7169 5.3536 3.0272 2.9-9.2
qBH-A01-1-2011IF2 8.91 2.3066 5.0922 2.7862 8.0-10.6
qBH-A01-2-2011IF2 14.11 2.3209 4.8624 2.9737 13.5-15.4
qBH-A03-2010DH 76.51 2.5367 6.7539 4.3702 68.6-79.5
qBH-A09-2011DH 58.91 -3.0668 5.9761 3.9711 57.5-59.5
qBH-C01-1-2010DH 31.71 3.9053 16.5377 7.1960 30.7-32.5
qBH-C01-2011DH 26.41 -3.5477 7.1231 4.0033 25.3-27.6
qBH-C01-2011IF2 29.31 -1.7200 4.0073 2.5198 27.7-30.7
qBH-C01-2-2010DH 37.01 3.8418 15.6741 6.6958 36.5-37.1
qBH-C03-1-2010DH 17.61 2.7300 6.8083 4.9960 15.0-18.6
qBH-C03-2-2010DH 24.81 3.6189 10.7972 7.3851 22.3-25.9
qBH-C03-3-2010DH 30.11 2.9942 7.7415 4.6107 28.1-33.5
qBS-A07-2011DH 6.31 -4.1923 5.2783 3.0509 2.7-8.8
qBS-A09-2010DH 10.51 4.5982 8.0987 3.3536 6.5-12.1
qBS-C03-2011IF2 12.61 -2.0515 7.4363 4.5989 11.0-13.1
qBS-C04-2010DH 11.31 -3.7725 4.7862 2.7486 3.8-17.9
qAIL-A07-2011DH 5.31 -0.5257 4.2949 2.7073 3.3-10.1
qAIL-C05-2010IF2 3.81 -0.3883 6.0439 2.8258 2.9-7.1
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