亚洲棉短绒突变体纤维发育及其差异基因表达分析

doi:10.3724/SP.J.1006.2020.94133

Abstract

Abstract:

Cotton fiber, one of main nature textile and longest single cell, is a good material for researching the cotton fiber development. In this study, fuzz mutant (FZ) and wild-type (fz) which belong to diploid Gossypium arboreum species were used for unraveling molecular mechanism of fuzz fiber initiation by scanning electron microscopy (SEM), paraffin section and RNA sequencing technology. It was found that any fibers scarcely initiated on the ovule surface of the mutant at 0DPA, compared with that of the wild-type. Only lint fiber cells emerged on the ovule surface of fuzz mutant, while abundant fuzz and lint fiber cells appeared in wild-type at +3DPA stage. The transcriptome analysis of FZ and fz across the four fiber development stages (0DPA, +3DPA, +5DPA, and +8DPA) indicated that the number of differentially expressed gene was 3780. There were litter differentially expressed genes at 0DPA in FZ and fz. In the proceed of ovule development, the number of differentially expressed genes was increased gradually. The KEGG enriched pathway analysis illustrated that these differentially expressed genes were involved in cutin, suberine and wax biosynthesis, phenylalanine metabolism pathways and plant hormone signal transduction pathways. The analysis of co-expressed gene trend lines indicated that the up-regulated genes in mutant at +3DPA were all involved in metal ion binding, MAPK cascade, oxidoreductase activity and transcription regulation, resulting in failed fuzz fiber cells initiation in mutant. These findings elaborated a dynamic variation about fuzz fiber initiation in diploid G. arboreum, which provides a reference for further research in the cotton fiber development.

Key words: Gossypium arboreum, fuzzless mutant, differentially expressed gene, co-expressed trend

Xiao-Yang WANG,Li-Yuan WANG,Zhao-E PAN,Shou-Pu HE,Xiao WANG,Wen-Fang GONG,Xiong-Ming DU. Analysis of differentially expressed genes and fiber development in Gossypium arboreum fuzzless mutant[J].Acta Agronomica Sinica, 2020, 46(5): 645-660.

Figures/Tables 13

Table 1

Primers used for real-time quantitative PCR verify of cotton differentially expressed genes (DEGs)"

基因编号 Gene	正向引物 Forward primer (5'-3')	反向引物 Reverse primer (5'-3')	log₂RT-QPCR	log₂FPKM	功能注释 Functional annotations
Ga02G0173	GTGCTCGGAAGAAAACGCTC	CGCACCTGCCAAAGTTGTTA	1.31	1.10	谷胱甘肽转移酶 Glutathione S-transferase
Ga03G0324	ACAGCAAAAGGCAATCGAGAAC	CGTGGGAAGCTATCGGCTTT	2.85	1.65	谷胱甘肽转移酶 Glutathione S-transferase
Ga04G0014	CTTTCGGGTCAGGTCGAAGG	GTTGTGTTACTCGGGGGACC	3.52	2.89	细胞色素氧化酶 Cytochrome P450
Ga05G3679	GAGCCATCGCTAAGTTCTGGGC	TCCAACAATTTCAGGCACGCAA	-1.19	-1.40	谷胱甘肽转移酶 Glutathione S-transferase
Ga05G3965	CCTGCTATTGCCATCAACACTT	TTGTGCTGGACTTGTTCTTGCT	-2.00	-1.24	分子伴侣 Molecular chaperone
Ga07G0568	CAACGGCGAAAACAACACGGAA	TGCACTGGTCTCCTTCTGTGGA	1.31	1.18	甲基转移酶Methyltransferase
Ga07G1530	GGTGGTAACCCTAGCTTGTGGC	GCGCGTTATGTGTAACTTGCGA	1.26	1.11	转录因子WD40
Ga07G1742	GCCGTGCTAACTTATTCCCTTGC	AGCACCCCAAGTAGTAACCAAGC	-1.54	-1.20	钙联蛋白 Calnexin 1
Ga07G2088	TTGCATCAGAGCAGCCTGCATA	TCTGCTGCCCAAGATTTGCCAT	-1.54	-1.32	转录因子 MYB108
Ga07G2611	GCAGAGGCAGCTTTAAGTTTGGG	GCATCCCACCAGGCCTAAGTTC	-1.61	-1.55	网状蛋白 Reticulon-like protein
Ga08G0121	TGTCTTCGGAACCCGGAGATGA	GGCATCCCACTAGCAGCATTGA	4.47	6.69	蛋白受体激酶 Receptor-like protein kinase
Ga09G0409	TACCGGTGCACTGATCGACCTA	TTCTGAGTCGACTGTTCGTCGC	2.03	1.55	功能未知 Unknown
Ga09G0435	TGCAGGGTTGTTAAGGTGTGGG	TGATTTCGTTATCGGTCCGCCC	1.14	1.05	假定蛋白 Predicted protein
Ga09G0661	CGCCATGCTTGCCATCTTCATC	CACTGAAGGCAGCTTGATCCGA	1.28	1.31	还原酶 Dihydroflavonol-4-reductase
Ga10G0810	ATGAGCCGCCCTATTGACTGCC	TGGTAGATGCCTTCGTTGCTGA	2.24	2.51	RNA加工 RNA processing
Ga10G1419	CCAACAAGGGCCCAAGTGCTAT	TCTTGTTCCCTTGGCCGACTTG	-1.22	-1.20	NADH脱氢酶 NADH dehydrogenase
Ga11G2832	TAAGATCGGTTCGGCTTCGG	ATGCTCCACCCTAACTTGCC	2.01	2.45	假定蛋白 Hypothetical protein
Ga11G2833	CACTTCTCGTGTGATGACCTGT	CATCTTCTAACGCTGGAATCTG	3.70	5.20	谷胱甘肽转移酶 Glutathione S-transferase
Ga11G3625	ATGCTTCTCAACTGCGCAGGAA	GCTGTTGTCGAACTTTATGGGGC	2.45	2.86	谷胱甘肽转移酶 Glutathione S-transferase
Ga11G3670	GACCGATGAAGAGGAACCCAGC	CCATGTGGTGTCCCGGTATGAC	-2.88	-1.78	NADH脱氢酶 NADH dehydrogenase
Ga12G0113	TCGACGTCGCCCTGTTTTTCAT	CTGACGTGGACGCCATGTTGAT	1.53	1.58	假定蛋白 Hypothetical protein
Ga10G0013	TACCAGTTGAAGCCGAAGGCTG	CCATTGTAAGGCCTGCGCTTTG	2.18	1.81	甲基转移酶N-methyltransferase

Table 1

Fig. 1

Table 2

Analysis of transcriptome data and comparison with reference genome"

编号 No	过滤后的序列数 Clean reads	GC GC (%)	Q30 Q30 (%)	原始数 Total reads	比对到参考基因组的总数 Mapped reads	比对到参考基因组唯一位置的数目 Uniq mapped reads	比对到参考基因组多个位置的数目 Multiple mapped reads	比对到正链数 Reads map to “+”	比对到负链数 Reads map to “-“
L01	6E+07	42.75	88.41	1.13E+08	100098347(88.79%)	95615963 (84.82%)	4482384 (3.98%)	49356770 (43.78%)	49542917 (43.95%)
L02	7E+07	42.89	87.89	1.49E+08	132564763(88.84%)	126665191 (84.88%)	5899572 (3.95%)	65411403 (43.84%)	65613688 (43.97%)
L03	6E+07	43.73	88.21	1.29E+08	114781600(89.23%)	109876069 (85.42%)	4905531 (3.81%)	56731466 (44.10%)	56901283 (44.24%)
L04	7E+07	43.32	87.93	1.31E+08	115706679(88.26%)	109946001 (83.86%)	5760678 (4.39%)	57037850 (43.51%)	57182987 (43.62%)
L05	6E+07	43.45	88.18	1.15E+08	101447417(88.47%)	96871049 (84.47%)	4576368 (3.99%)	50115129 (43.70%)	50235739 (43.81%)
L06	7E+07	43.65	88.41	1.36E+08	119977298(88.23%)	114195412 (83.98%)	5781886 (4.25%)	59276583 (43.59%)	59413894 (43.69%)
L07	9E+07	43.47	88.41	1.85E+08	164355512(88.86%)	156168989 (84.43%)	8186523 (4.43%)	81092741 (43.84%)	81289251 (43.95%)
L08	6E+07	43.21	87.12	1.24E+08	108363137(87.68%)	102760244 (83.15%)	5602893 (4.53%)	53406006 (43.21%)	53552268 (43.33%)
L09	6E+07	43.53	88.53	1.25E+08	110741117(88.61%)	105077371 (84.08%)	5663746 (4.53%)	54559214 (43.66%)	54711263 (43.78%)
L10	6E+07	43.93	88.53	1.12E+08	95982638(85.64%)	89277314 (79.66%)	6705324 (5.98%)	47006407 (41.94%)	47142478 (42.06%)
L11	6E+07	43.62	87.74	1.24E+08	106630817(85.76%)	99506038 (80.03%)	7124779 (5.73%)	52250173 (42.03%)	52415958 (42.16%)
L12	7E+07	43.65	88.90	1.45E+08	125095168(86.41%)	116813022 (80.69%)	8282146 (5.72%)	61391019 (42.41%)	61584013 (42.54%)
L13	6E+07	43.78	89.76	1.28E+08	109195486(85.27%)	104512429 (81.62%)	4683057 (3.66%)	53869063 (42.07%)	54027240 (42.19%)
L14	7E+07	43.40	90.20	1.31E+08	115537224(88.07%)	110898150 (84.53%)	4639074 (3.54%)	57057026 (43.49%)	57220578 (43.61%)
L15	8E+07	43.20	90.60	1.63E+08	144883755(88.62%)	139117996 (85.09%)	5765759 (3.53%)	71628619 (43.81%)	71848062 (43.94%)
L16	7E+07	44.19	89.70	1.44E+08	122786455(85.34%)	116535579 (80.99%)	6250876 (4.34%)	60495477 (42.04%)	60621983 (42.13%)
L17	8E+07	44.14	90.20	1.56E+08	130032853(83.13%)	123871138 (79.19%)	6161715 (3.94%)	64115657 (40.99%)	64295623 (41.10%)
L18	7E+07	43.38	90.49	1.44E+08	127237888(88.61%)	121687992 (84.75%)	5549896 (3.87%)	62876344 (43.79%)	63029624 (43.90%)
L19	6E+07	43.32	91.03	1.19E+08	106495539(89.28%)	101489480 (85.08%)	5006059 (4.20%)	52555236 (44.06%)	52649155 (44.14%)
L20	6E+07	43.38	91.29	1.16E+08	101384931(87.60%)	96799488 (83.64%)	4585443 (3.96%)	50050156 (43.24%)	50157927 (43.34%)
L21	6E+07	43.63	91.17	1.12E+08	98144438(87.78%)	93630910 (83.74%)	4513528 (4.04%)	48444159 (43.33%)	48541604 (43.42%)
L22	6E+07	44.24	87.94	1.22E+08	100479675(82.20%)	92865206 (75.98%)	7614469 (6.23%)	49048631 (40.13%)	49206041 (40.26%)
L23	6E+07	44.06	87.32	1.23E+08	102829882(83.46%)	95769710 (77.73%)	7060172 (5.73%)	50222205 (40.76%)	50359879 (40.87%)
L24	6E+07	44.39	86.86	1.14E+08	96749028(84.55%)	90393952 (79.00%)	6355076 (5.55%)	47365502 (41.39%)	47485322 (41.50%)

Table 2

Fig. 2

Fig. 3

Table 3

Ninety-one common significant DEGs found in fz and FZ across fiber development stages and their expression"

基因编号 Gene ID	基因注释Gene annotation	0DPA	+3DPA	+5DPA	+8DPA
Ga11G2831	含有WD40结构的蛋白 WD40 repeat-like superfamily protein	2.00	2.70	3.00	1.72
Cotton_newGene_517935	假定蛋白 Hypothetical protein	2.55	2.94	1.88	1.84
Ga03G0324	谷胱甘肽S-转移酶 Glutathione S-transferase	1.33	1.65	1.63	1.05
Ga11G0115	假定蛋白 Hypothetical protein	-1.25	-1.28	-1.42	-1.05
Ga07G1809	硫代硫酸盐ATP结合蛋白 Sulfate/thiosulfate ATP-binding protein	2.35	2.34	2.40	0.95
Cotton_newGene_190500	未鉴定蛋白 Uncharacterized protein	2.37	2.67	0.97	1.16
Cotton_newGene_217366	未鉴定蛋白 Uncharacterized protein	2.03	2.44	1.33	1.65
Ga11G3311	葡萄糖基转移酶 UDP-glycosyltransferase 83A1	1.25	1.98	1.50	1.85
Ga09G2202	乙烯响应因子 AP2/ERF	1.95	1.31	2.13	-1.42
Ga09G0608	亮氨酸拉链蛋白 Leucine-rich repeat protein kinase	-1.90	-1.49	-0.70	-0.20
Cotton_newGene_190685	假定蛋白 Hypothetical protein	1.39	1.60	0.94	0.89
Cotton_newGene_184413	假定蛋白 Hypothetical protein	0.86	0.82	0.68	0.20
Cotton_newGene_216530	假定蛋白 Hypothetical protein	1.42	1.92	0.70	1.48
Ga06G0475	泛素连接酶 E3 ligase	-0.74	-0.80	-0.53	-0.61
Cotton_newGene_206595	假定蛋白 Hypothetical protein	0.62	0.51	0.22	0.08
Cotton_newGene_188033	假定蛋白 Hypothetical protein	-3.18	-2.89	-2.89	-1.50
Ga14G2119	谷氨酸受体 Glutamate receptor	3.94	3.27	3.11	1.92
Cotton_newGene_206588	假定蛋白 Hypothetical protein	2.13	2.00	1.73	0.92
Ga11G2832	自噬相关蛋白 Autophagy-related protein	2.42	2.45	2.66	1.38
Ga06G1674	锌依赖的包外多肽酶蛋白 Zn-dependent exopeptidases protein	-1.22	-0.78	-0.75	-0.57
Cotton_newGene_313214	TMV抗性蛋白 TMV resistance protein	0.42	1.14	1.27	0.73
Ga04G1219	乙酰基葡萄糖转移酶 UDP-GlcNAc	1.21	1.37	1.40	0.78
Cotton_newGene_383086	ATP合成酶 ATP synthase	1.29	1.00	1.15	0.57
Cotton_newGene_336363	核酸内切酶 Endonuclease	1.47	1.00	1.30	0.46
Cotton_newGene_521387	功能未知 Unknown	1.87	1.81	1.73	1.36
Ga04G0915	功能未知 Unknown	1.43	1.30	1.23	0.32
Cotton_newGene_190683	细胞色素氧化酶 P450 Cytochrome P450	2.78	3.25	2.09	1.73
Cotton_newGene_94861	核酸酶 Putative nuclease	-1.00	-0.86	-0.93	-0.65
Cotton_newGene_195900	功能未知 Unknown	-3.47	-3.50	-3.45	-2.85
Ga10G0013	甲基转移酶 Histone-lysine N-methyltransferase	1.78	1.81	2.10	1.35
Ga11G0121	木糖梅 Alpha-xylosidase 1	-1.64	-0.77	-0.92	-1.26
Ga11G0187	假定蛋白 Hypothetical protein	-0.85	-0.74	-0.81	-0.48
Ga11G0110	假定蛋白 Hypothetical protein	0.82	1.21	0.71	0.84
Ga06G1755	脯氨酸受体蛋白激酶Proline-rich receptor-like protein kinase	-1.00	-0.52	-0.98	-0.95
Ga05G2954	天冬氨酸酶 Asparticase	0.65	0.58	1.25	0.24
Cotton_newGene_572274	假定蛋白 Hypothetical protein	2.24	2.62	1.48	1.69
Cotton_newGene_353692	假定蛋白 Hypothetical protein	-0.56	-0.63	-0.71	-0.49
Cotton_newGene_483966	假定蛋白 Hypothetical protein	2.77	2.77	2.71	2.30
Ga11G0111	微丝蛋白 Fb17	-0.31	-0.75	-1.21	-2.11
Cotton_newGene_99176	丝氨酸/苏氨酸蛋白磷酸酶 Serine/threonine protein phosphatase	1.08	0.85	0.97	1.10
Ga12G1137	乙烯受体蛋白 AP2/ERF	1.25	1.53	1.81	-1.25
Ga11G2354	假定蛋白 Hypothetical protein	1.73	1.58	1.68	0.31
Cotton_newGene_517938	细胞色素氧化酶 P450 Cytochrome P450	4.11	4.65	3.60	3.14
Cotton_newGene_300782	核酸内切酶亚基 Endonuclease subunit	1.47	1.44	1.06	1.14
Cotton_newGene_252500	Copia型多蛋白 Copia-type polyprotein	2.74	2.88	2.52	2.00
Cotton_newGene_517934	功能未知 Unknown	3.19	3.84	2.65	1.73
Cotton_newGene_353671	缬氨酸-tRNA连接酶 Valine-tRNA ligase	-0.39	-0.95	-0.65	-0.94
Ga05G3965	功能未知 Unknown	-1.55	-1.24	-1.95	-1.34
Ga09G1652	丝氨酸/苏氨酸蛋白磷酸酶 Serine/threonine protein phosphatase	-1.61	-1.34	-1.01	-1.25
Ga04G1773	转录因子 NAC1	1.20	0.98	2.02	-1.43
Ga09G1364	乙酰辅酶A氧化酶 Acyl-coenzyme A oxidase	2.66	2.52	2.53	2.06
Cotton_newGene_213765	假定蛋白 Hypothetical protein	-1.28	-1.29	-1.89	-1.22
Ga04G0280	富含脯氨酸蛋白 Proline-rich protein	0.71	0.77	1.46	1.21
Cotton_newGene_217374	假定蛋白 Hypothetical protein	1.56	1.89	0.72	1.26
Ga10G0798	细胞壁受体激酶 Wall-associated receptor kinase	0.44	0.41	0.30	0.13
Ga03G0640	功能未知 Unknown	1.88	1.62	1.14	1.16
Cotton_newGene_577041	功能未知 Unknown	3.08	1.66	3.58	0.40
Ga07G1742	含有CBS结构域的蛋白 CBS domain-containing protein	-1.45	-1.20	-1.17	-0.69
Ga07G2703	丝氨酸/苏氨酸蛋白磷酸酶 Serine/threonine-protein kinase	-0.81	-0.59	-0.39	-0.26
Ga07G0566	转录因子 bHLH137	1.05	1.03	3.13	-0.50
Cotton_newGene_190686	假定蛋白 Hypothetical protein	1.42	1.61	0.84	0.86
Ga04G1342	未鉴定蛋白 Uncharacterized protein	0.80	0.30	0.17	0.37
Cotton_newGene_563611	未鉴定蛋白 Uncharacterized protein	2.35	2.52	1.44	1.83
Cotton_newGene_592029	丝裂原活化蛋白激酶 Mitogen-activated protein kinase MMK1	-1.65	-1.23	-0.87	-0.46
Ga01G1687	富含半胱氨酸/组氨酸蛋白 Cysteine/histidine-rich protein	0.74	0.58	0.54	0.48
Cotton_newGene_54250	功能未知 Unknown	2.88	2.87	2.67	1.88
Cotton_newGene_353710	假定蛋白 Hypothetical protein	1.50	1.25	1.10	1.44
Cotton_newGene_474685	未鉴定蛋白 Uncharacterized protein	2.45	2.75	1.40	1.79
Cotton_newGene_67170	假定蛋白 Hypothetical protein	3.06	2.61	2.59	1.44
Cotton_newGene_353674	芳香类多肽 Pentafunctional arom polypeptide	1.41	1.31	1.33	1.02
Ga03G1055	木聚糖糖基化酶 Xyloglucan endotransglucosylase	0.83	2.34	-1.71	0.96
Ga11G0123	甲硫氨酸tRNA转移酶 Methionine-tRNA ligase	-2.16	-1.98	-1.73	-1.21
Ga07G0272	咖啡酸盐酯酶 Caffeoylshikimate esterase	-1.59	-1.52	-1.82	-1.20
Cotton_newGene_57872	假定蛋白 Hypothetical protein	0.51	0.71	0.36	0.36
Ga03G2383	受体蛋白 Receptor	-0.81	-1.43	-2.23	1.21
Ga02G0173	假定蛋白 Hypothetical protein	1.44	1.10	1.19	1.04
Cotton_newGene_509534	假定蛋白 Hypothetical protein	1.82	2.28	1.03	1.35
Ga11G0124	未鉴定蛋白 Uncharacterized protein	-2.92	-4.04	-3.64	-3.84
Ga13G1311	丝氨酸/苏氨酸蛋白磷酸酶 Serine/threonine-protein phosphatase	1.80	1.60	1.61	1.42
Ga10G1419	NADH脱氢酶 NADH dehydrogenase	-0.99	-1.20	-1.37	-1.31
Cotton_newGene_190499	未鉴定蛋白 Uncharacterized protein	1.30	1.60	0.80	0.91
Cotton_newGene_217363	假定蛋白 Hypothetical protein	1.69	2.16	0.97	1.75
Ga07G2215	未鉴定蛋白 Uncharacterized protein	1.38	2.23	-2.23	0.98
Ga11G2833	谷胱甘肽S-转移酶 Glutathione S-transferase	4.06	5.20	5.45	1.92
Cotton_newGene_473974	功能未知 Unknown	1.76	1.56	1.46	0.69
Ga07G0353	富含脯氨酸蛋白 Proline-rich protein	1.48	1.61	1.06	0.36
Cotton_newGene_99000	假定蛋白 Hypothetical protein	0.73	0.61	0.46	0.92
Ga07G1792	CBL9	-1.33	-1.33	-1.07	-0.68
Cotton_newGene_190497	假定蛋白 Hypothetical protein	2.55	2.89	2.28	1.86
Ga09G1540	蛋白YLS9 YLS9 protein	0.88	1.12	-0.82	0.66
Ga09G1216	川芎嗪-3 Tetraspanin-3	-1.02	-1.53	-0.94	-2.15

Table 3

Fig. 4

Table 4

DEGs functioning in cutin, suberine and wax biosynthesis, and phenylalanine metabolism pathways and their expression"

基因编号Gene ID	基因功能 Gene function	0DPA	+3DPA	+5DPA	+8DPA	KEGG_pathway_annotation
Ga01G2133	ω-羟基棕榈酸酯-乙酰酰转移酶 Omega-hydroxypalmitate-feruloyl transferase	0.05	0.52	-0.11	1.07	Cutin, suberine, and wax biosynthesis
Ga12G0500	细胞色素氧化酶 P450 Cytochrome P450	-0.06	0.18	-1.22	-1.24	Cutin, suberine, and wax biosynthesis
Ga07G2444	细胞色素氧化酶 P450 Cytochrome P450	0.06	1.06	-0.18	-0.40	Cutin, suberine, and wax biosynthesis
Ga08G1573	细胞色素氧化酶 P450 Cytochrome P450	-0.81	-0.68	-0.63	1.02	Cutin, suberine, and wax biosynthesis
Ga08G2220	细胞色素氧化酶 P450 Cytochrome P450	-1.95	-2.11	-2.22	0.14	Cutin, suberine, and wax biosynthesis
Ga10G2228	ω-羟基棕榈酸酯-乙酰酰转移酶 Omega-hydroxypalmitate-feruloyl transferase	0.59	-1.55	0.32	0.59	Cutin, suberine, and wax biosynthesis
Ga04G1297	脂肪酸羟化酶 Fatty acid hydroxylase	-0.95	-0.15	0.31	2.10	Cutin, suberine, and wax biosynthesis
Ga07G2443	细胞色素氧化酶 P450 Cytochrome P450	0.05	1.16	-0.09	-0.37	Cutin, suberine, and wax biosynthesis
Ga05G2018	过氧化物酶Peroxidase	0.34	0.88	1.20	-0.25	Phenylalanine metabolism
Ga14G0143	肉桂醇脱氢酶 Cinnamyl alcohol dehydrogenase	-0.06	0.47	0.77	-1.41	Phenylpropanoid biosynthesis
Ga10G0561	甲基转移酶 Caffeic acid-methyltransferase	0.08	-0.02	-0.19	-1.30	Phenylalanine metabolism
Ga11G3625	过氧化物酶 Peroxidase	0.65	2.86	1.37	1.11	Phenylalanine metabolism
Ga12G1737	甲基转移酶 Caffeic acid-methyltransferase	-1.18	-0.54	-0.96	1.15	Phenylpropanoid biosynthesis
Ga05G1817	过氧化物酶 Peroxidase	-0.05	0.33	-0.17	-2.71	Phenylalanine metabolism
Ga12G2843	过氧化物酶 Peroxidase	-1.71	-1.17	-0.29	-0.02	Phenylalanine metabolism
Ga12G0336	甲基转移酶 Caffeic acid-methyltransferase	0.35	0.44	-0.05	-1.12	Phenylpropanoid biosynthesis
Ga03G0366	花青素葡萄糖基转移酶 Anthocyanidin-glucosyltransferase	0.25	0.72	-0.22	1.45	Phenylpropanoid biosynthesis
Ga05G0239	乙烯脱氢酶 Aldehyde dehydrogenase	-0.01	0.02	0.37	-3.41	Phenylpropanoid biosynthesis
Ga09G0681	葡萄糖苷酶 Glucosidase	-0.18	0.30	-0.15	-1.20	Phenylpropanoid biosynthesis
Ga04G1983	过氧化物酶 Peroxidase	0.08	0.33	0.23	-1.16	Phenylalanine metabolism
Ga09G1700	苯丙氨酸氨裂合酶 Phenylalanine ammonia-lyase	-0.19	0.37	-1.21	-0.84	Phenylalanine metabolism
Ga01G2475	过氧化物酶 Peroxidase 68	0.42	2.69	0.60	-0.49	Phenylalanine metabolism
Ga10G0968	肉桂酸4-羟化酶 Cinnamate 4-hydroxylase	0.24	-0.26	-1.28	-0.01	Phenylalanine metabolism
Ga11G1671	过氧化物酶 Peroxidase 17	0.13	0.46	0.05	-2.06	Phenylalanine metabolism
Ga10G1786	过氧化物酶 Cationic peroxidase 1	0.46	1.38	0.50	-0.25	Phenylalanine metabolism
Ga10G1772	莽草酸O-羟基肉桂酰基转移酶 Shikimate O-hydroxycinnamoyltransferase	0.04	1.14	1.78	1.38	Phenylpropanoid biosynthesis
Ga11G2088	过氧化物酶 Peroxidase 72	0.42	0.22	0.79	-2.38	Phenylalanine metabolism
Ga13G0640	细胞色素氧化酶 P450 Cytochrome P450	-0.09	0.15	0.12	-1.35	Phenylpropanoid biosynthesis
Ga12G1739	氨基甲酸N-甲基转移酶 Anthranilate N-methyltransferase	-1.30	-0.86	-0.68	0.34	Phenylpropanoid biosynthesis
Ga04G0842	苯丙氨酸裂解酶 Phenylalanine ammonia-lyase	-0.12	0.06	-1.08	-0.88	Phenylalanine metabolism
Ga04G1242	过氧化物酶 Peroxidase 51	0.44	1.08	-0.02	0.17	Phenylalanine metabolism

Table 4

Table 5

DEGs in plant hormone signal transduction pathways and their expression"

基因编号 Gene ID	基因注释 Gene annotation	基因名称Gene name	激素Hormone	0DPA	+3DPA	+5DPA	+8DPA
Ga06G1125	乙烯响应因子 EIN3-binding F-box protein 1	EIN3	ETH	1.1	1.1	4.6	0.4
Ga01G1781	生长素响应因子Auxin response factor 9	ARF9	IAA	1.0	1.2	2.4	0.6
Ga09G2536	生长素诱导蛋白Auxin-induced protein 22D	AUX22D	IAA	1.6	1.0	2.6	1.6
Ga02G1198	脱落酸不敏感蛋白Abscisic acid-insensitive protein	ABI3	ABA	0.7	0.8	2.1	0.4
Ga01G2735	转录因子MYC4 Transcription factor MYC4	MYC4	JA	1.7	1.4	4.1	0.5
Ga13G1098	生长素诱导蛋白Auxin-induced protein X10A	AIP10A	IAA	1.0	1.0	0.6	2.2
Ga03G2153	吲哚乙酸合成酶Indole-3-acetic acid-amido synthetase	IAA	IAA	0.9	5.0	2.7	0.6
Ga05G2728	生长素诱导蛋白Auxin-induced protein 6B	AIP6B	IAA	1.2	0.9	3.2	1.0
Ga11G1087	生长素响应蛋白Auxin-responsive protein IAA16	IAA16	IAA	0.8	1.1	4.2	0.4
Ga07G0242	脱落酸受体Abscisic acid receptor	PYL2	ABA	1.2	1.7	1.5	0.7
Ga01G0771	吲哚乙酸合成酶 Indole-3-acetic acid-amido synthetase	IAA	IAA	1.4	1.3	3.0	0.5
Ga08G2804	乙烯响应因子 Ein3-binding F-box protein 4	EIN3	ETR	1.4	1.2	4.6	0.7
Ga06G2183	磷酸转移蛋白Histidine-containing phosphotransfer protein 4	AHP4	CK	1.0	1.0	0.6	0.4
Ga10G2395	生长素响应蛋白 Auxin-responsive protein IAA26	SRK2I	IAA	0.8	1.4	2.3	0.7
Ga10G3077	生长素诱导蛋白Auxin-induced protein X15	AIPX15	IAA	0.3	0.4	0.7	1.0
Ga08G2252	生长素响应蛋白Auxin-responsive protein IAA11	IAA11	IAA	1.2	0.9	2.5	0.6
Ga03G2608	生长素诱导蛋白Auxin-induced protein X10A	AIP10A	IAA	2.2	0.8	1.3	1.0
Ga03G0428	乙烯受体Ethylene receptor 2	ETR2	ETH	1.3	0.9	2.6	0.7
Ga11G1046	乙烯受体Ethylene receptor	EIN4	ETH	1.3	1.2	2.2	0.8
Ga07G0142	蛋白磷酸酶 Protein phosphatase 2C	PP2C	ABA	1.5	1.6	4.0	0.8
Ga05G0163	乙烯响应蛋白EIN3-binding F-box protein 1	EIN3	ETH	0.9	1.2	3.6	0.4
Ga09G2537	生长素响应蛋白 Auxin-responsive protein IAA14	IAA14	IAA	1.0	0.5	0.4	1.2
Ga12G2846	赤霉素受体 Gibberellin receptor GID1B	GID1B	GA	1.2	1.8	2.1	0.4
Ga08G2885	蛋白磷酸酶 Protein phosphatase 2C	PP2C77	ABA	1.4	1.3	3.9	0.4
Ga07G0455	生长素响应蛋白 Auxin-responsive protein IAA9	IAA9	IAA	1.6	0.8	2.6	0.3
Ga03G2632	生长素转运蛋白 Auxin transporter-like protein 5	ATP5	IAA	0.9	1.1	2.3	0.8
Ga11G3045	脱落酸受体 Abscisic acid receptor	PYL9	ABA	1.6	1.2	4.2	0.4
Ga11G1086	生长素诱导蛋白 Auxin-induced protein 22D	AUX22D	IAA	1.3	1.8	6.4	0.5
Ga03G2592	生长素诱导蛋白 Auxin-induced protein 15A	AUX15A	IAA	0.9	0.6	0.5	2.2
Ga05G1344	生长素响应蛋白 Auxin-responsive protein IAA16	IAA16	IAA	1.5	1.0	2.3	0.6
Ga05G2981	细胞分裂素响应蛋白 Cytokinin ARR17	ARR17	IAA	1.0	1.2	1.0	0.5
Ga11G2673	乙烯响应转录因子 Ethylene-responsive transcription factor	ERF10	ETH	1.8	1.0	15.7	0.1
Ga12G2331	ABA相关蛋白激酶 ABA-related protein kinase	SNF1	ABA	1.2	1.3	2.1	0.9
Ga05G0157	生长素响应蛋白IAA29 Auxin-responsive protein IAA29	IAA29	IAA	1.3	0.8	0.5	0.5
Ga02G1269	水杨酸相关蛋白 Salicylic acid-related protein	PRP1	SA	0.8	0.9	0.4	0.5
Ga06G2185	磷酸转移蛋白 Histidine phosphotransfer protein	AHP3	CK	1.0	0.9	0.7	0.5
Ga13G2114	生长素诱导蛋白Auxin-induced protein 22B	AUX15A	IAA	1.4	1.2	2.1	1.9
Ga05G3500	生长素诱导蛋白 Auxin-induced protein X15	AUX15	IAA	3.6	0.7	0.9	0.6
Ga07G1535	吲哚乙酸合成酶 Indole-3-acetic acid-amido synthetase	IAA	IAA	0.8	1.3	1.5	0.4
Ga09G2560	乙烯受体 Ethylene receptor 2	ETR2	ETH	1.3	1.4	6.3	0.3
Ga03G2582	生长素诱导蛋白 Auxin-induced protein 15A	AUX15A	IAA	1.0	0.9	2.1	0.8
Ga05G1383	生长素响应蛋白 Auxin-responsive protein IAA27	IAA27	IAA	1.1	1.1	1.3	0.2
Ga11G1865	生长素响应蛋白 Auxin-responsive protein	IAA	IAA	1.3	0.9	2.0	0.4
Ga05G1988	生长素诱导蛋白 Auxin-induced protein 6B	AUX6B	IAA	1.1	0.8	2.9	0.3

Table 5

Fig. 5

Fig. 6

Fig. 7

Fig. 8

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