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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (08): 1378-1388.doi: 10.3724/SP.J.1006.2011.01378

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Gene Expression Profiles in Pod and Leaf of Two Major Peanut Cultivars in Southern China

CHEN Xiao-Ping,ZHU Fang-He,HONG Yan-Bin,LIU Hai-Yan,ZHANG Er-Hua,ZHOU Gui-Yuan,LI Shao-Xiong,ZHONG Ni,WEN Shi-Jie,LI Xing-Yu,LIANG Xuan-Qiang*   

  1. Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
  • Received:2011-01-15 Revised:2011-04-27 Online:2011-08-12 Published:2011-06-13
  • Contact: 梁炫强, E-mail: Liang804@yahoo.com; Tel: 020-87597315

Abstract: Great improvements have been achieved in peanut yield, quality and resistance through traditional breeding methods. However, variations in gene expression profiles in major cultivars are yet unclear. Here, we utilized a high-density peanut oligonucleotide microarray to analyze gene expression profiles in pod and leaf of Shanyou 523 and Yueyou 7, which are widely grown in southern China. The results indicated that 1 383 differentially expressed genes were detected in pod, 662 and 721 of which were up- and down-regulated, respectively, while 647 differentially expressed genes were detected in leaf, 234 and 413 of which were up- and down-regulated, respectively. Among the pod differentially expressed genes, 52 and 105 genes were, respectively, up- and down-regulated at least 10-fold, whereas only two and five were found in leaf. To further characterize these differentially expressed genes, we used Gene Ontology (GO) for annotation of them. The results showed that a large proportion of differentially expressed genes from both pod and leaf were distributed in cell and membrane, possessed binding and catalytic activities and were involved in metabolic and cellular processes. Additionally, the expression of four differentially expressed genes was validated by real time qRT-PCR, further confirming the microarray results.

Key words: Cultivated peanut (Arachis hypogaea L.), Microarray, Gene expression profiles, Differentially expressed genes, Real-time quantitative RT-PCR

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