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Acta Agron Sin ›› 1994, Vol. 20 ›› Issue (01): 33-38.

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The Variation of Chloroplast DNA and Mitochondrial DAN in Tissue Culture of Alfalfa

He Qi-qian.;Liu Xin-zhi   

  1. Beijing Agricultural College, Beijing,102208
  • Received:1992-05-01 Revised:1992-08-17 Online:1994-01-12 Published:1994-01-12
  • Contact: He Qi-qian

Abstract: Chloroplast DNA and mitochondrial DNA were isolated form leaves and callus of alfalfa, Those DNAs were digested with six restriction endonucleases (BamH1,Bg1 1, Bg1 II, Dra 1, EcoR1, HindIII), electrophoresed and transferred to nylon membianes. Southern blots were hybridized with sorghum probes cont

Key words: Alfalfa, Tissue, culture, Chloroplast DNA Mitochondrial DNA

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