Abstract:

The crop yield is affected seriously by the salty soil in Xinjiang. Cotyledons with petiole of the line of Brassica napus 1khp11 were transformed with strain of Agrobacterium tumefaciens EHA105, harboring Na⁺/H⁺ antiporter gene of Suaeda heteroptera in order to select the salt tolerant oilseed. The regeneratedly kan-resistant plants were obtained. In the research, major factors of transformation were discussed detailed. The results showed that the best physiological and transforming condition was preculture for 3 days with 1 mg/L 2,4-D for cotyledons with petiole and immersion in OD₆₀₀ 0.3–0.4 liquid bacterium for 7 min, 8%–9% kan–resistant green seedlings were obtained. It was proved that NHX gene was inserted into the genome of oilseed by the methods of PCR, Dot blot and PCR-Southern.

Key words: Agrobacterium tumefaciens, Cotyledons with petiole, Preculture, Concentration of bacterium, Immerged time, Transgenic oilseed