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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (1): 66-70.doi: 10.3724/SP.J.1006.2009.00066

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Analysis of Hemoglobin Gene in Cyanobacterium and Transformation into Brassica napus L.

TAN Xiao-Li1,2,KONG Fan-Ming1,*,ZHANG Li-Li1,CHEN Song2,QI Chui-Kou2*   

  1. 1Institute of Life Sciences,Jiangsu University,Zhenjiang 212013,China;2 Institute of Imdustrial Crop,Jiangsu Academy of Agricultural Sciences,Nanjiang 200014,China
  • Received:2008-03-24 Revised:2008-07-16 Online:2009-01-12 Published:2008-11-17
  • Contact: QI Chui-Kou

Abstract:

Hemoglobin exists in most organisms including animals, plants, protozoa, algae, bacteria and fungi. In the paper, hemoglobin gene SLR2097 was cloned from Synechocystis sp. PCC 6803, which is 375 bp in size and encodes123 amino acid polypeptide chains belonging to the truncated hemoglobin family. Hemoglobin canbind oxygen and co-ordinate the heme iron with protein ligation. Database search showed that a motif ‘F-[L]-x(4)-[G]-G-[T]-x(2)-[Y]-x-[G]-[R]-x-[M]-x(3)-H’ occurred in hemoglobins. Therefore, the truncated hemoglobins might evolve from the same ancestor of hemoglobin. The hemoglobin gene SLR2097 was ligated into binary vector pCAMBIA1300-CaMV35S, and then transformed into rapeseed cultivar Ningyou 12 with Agrobacterium mediated transformation method. Phenotype analysis indicated that the expression of hemoglobin gene SLR2097 led to the earlier maturity of transgenic rapeseed. These phenotype variations have a potential application in the rapeseed production in the future.

Key words: Agrobacterium tumefaciens, Brassica napus, Hemoglobin SLR2097, Transformation

[1] Hardison R C. A brief history of hemoglobins: Plant, animal, protist, and bacteria. Proc Natl Acad Sci USA, 1996, 93: 5675–5679
[2] Perutz M F. Regulation of oxygen affinity of hemoglobin: influence of structure of the globin on the heme iron. Ann Rev Biochem, 1979, 48: 327–386
[3] Perutz M F. Structure and function of hemoglobin. Harvey Lect, 1969, 63: 213–261
[4] Scott N L, Lecomte J T. Cloning, expression, purification and preliminary characterization of a putative hemoglobin from the cyanobacterium Synechocystis sp. PCC 6803. Protein Sci, 2000, 9: 587–597
[5] Pesce A, Couture M, Dewilde S, Guertin M, Yamauchi K, Ascenzi P, Moens L, Bolognesi M. A novel two-over-two α-helical sandwich fold is characteristic of the truncated hemoglobin family. Embo J, 2000, 19: 2424–2434
[6] Moens L, Vanfleteren J, Van de Peer Y, Peeters K, Kapp O, Czeluzniak J, Goodman M, Blaxter M, Vinogradov S. Globins in non-vertebrate species: Dispersal by horizontal gene transfer and evolution of the structure-function relationships. Mol Bio Evol, 1996, 13: 324–333
[7] Holmberg N, Lilius G, Bailey J E, Bulow L. Transgenic tobacco expressing Vitreoscilla hemoglobin exhibits enhanced growth and altered metabolite production. Nat Biotechnol, 1997, 15: 244–247
[8] Cardoza V, Stewart C N. Increased Agrobacterium-mediated transformation and rooting efficiencies in canola (Brassica napus L.) from hypocotyl segment explants. Plant Cell Rep, 2003, 21: 599–604
[9] Block M, Brouwer D, Tenning P. Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol, 1989, 91: 694–701
[10] Murashige T, Skong F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant, 1962, 15: 473–497
[11] Doyle J J. Isolation of plant DNA from fresh tissues. Focus, 1990, 12: 13–15
[12] Chomczynski P. Solubilization in formamide protects RNA from degradation, Nucl Acids Res, 1992, 20: 3791–3792
[13] Weber R E, Vinogradov S N. Nonvertebrate hemoglobins: functions and molecular adaptations. Physiol Rev, 2001, 81: 569–628
[14] Lecomte J T J, Scott N L, Vu B C, Falzone C J. Binding of ferric heme by the recombinant globin from the cyanobacterium Synechocystis sp. PCC 6803. Biochemistry, 2001, 40: 6541–6552
[15] Khosla C, Curtis J E, DeModena J, Rinas U, Bailey J E. Expression of intracellular hemoglobin improves protein synthesis in oxygen-limited Escherichia coli. Biotechnology, 1990, 8: 849–853
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