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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (09): 1280-1289.doi: 10.3724/SP.J.1006.2017.01280


Correlation Analysis of Sclerotinia Resistance with Lignin Content and Monomer G/S and its QTL Mapping in Brassica napus L.

CHEN Xue-Ping**,JING Ling-Yun**,WANG Jia,JIAN Hong-Ju,MEI Jia-Qin,XU Xin-Fu,LI Jia-Na,LIU Lie-Zhao*   

  1. College of Agronomy and Biotechnology, Southwest University / Chongqing Engineering Research Center for Rapeseed, Chongqing, 400715, China
  • Received:2017-01-07 Revised:2017-04-20 Online:2017-09-12 Published:2017-05-08
  • Contact: 刘列钊, E-mail: liezhao2003@126.com, Tel: 023-68251383 E-mail:1473718700@qq.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31371655) and the Science and Technology Committee of Chongqing (cstc2016shmszx80083).


Sclerotinia sclerotiorum is a fungal pathogen causing disease in a wide range of plants, resulting in serious damage in crop production. The detached stem inoculation assay of RIL and F2 populations at final flowering stage was conducted, near infrared (NIR) spectroscopy was used to measure lignin content and monomer G/S in the stem, and correlation analysis and QTL mapping for these traits were performed. The lesion size of the RIL had a significantly negative correlation with lignin content, with a correlation coefficient at –0.348 and –0.286 in 2013 and 2014, respectively. The monomer G/S was significantly correlated with lesion size in the RIL population, and the correlation coefficient at 0.198 and 0.167 in 2013 and 2014, respectively. The lesion size of F2 in 2014 was significantly and negatively correlated with lignin content in the stem, with a correlation coefficient at –0.306. The cross sections of resistant and susceptible plants from F2:3 family were stained with phloroglucinol-HCl, showing that the content of lignin was significantly lower in the less resistant materials than in more resistant plants. According to the high density SNP genetic maps and composite interval mapping, a total of 18 QTLs were identified, which were located on A04, A05, A06, A08, C01, C03, C04, C06, and C07 chromosomes, with the explained phenotypic variation by individual QTL ranging from 2.38% to 12.05% for nine QTLs of lesion size, from 2.03% to 13.75% for three QTLs of lignin content, and from 2.06% to 8.66% for six QTLs of monomer G/S. The research results provide some new insights for the Sclerotinia resistance breeding in B. napus.

Key words: Brassica napus, Sclerotinia sclerotiorum, Correlation, QTL, Lignin, Monomer G/S

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