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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (02): 179-189.doi: 10.3724/SP.J.1006.2017.00179


QTL Mapping for Seedling Dry Weight and Fresh Weight under Salt Stress and Candidate Genes Analysis in Brassica napus L.

HOU Lin-Tao**,WANG Teng-Yue**,JIAN Hong-Ju,WANG Jia,LI Jia-Na,LIU Lie-Zhao*   

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
  • Received:2016-04-20 Revised:2016-09-18 Online:2017-02-12 Published:2016-09-27
  • Contact: 刘列钊, E-mail: liezhao2003@126.com, Tel: 023-6825070
  • Supported by:

    The study was supported by the National Natural Science Foundation of China (31371655).


Salt stress is one of the main abiotic stresses affecting crop yield and it would be very important by using the salt tolerance related markers in rapeseed breeding to improve the oilseed production. In this research, the Brassica napus L high generation recombinant inbred lines (RIL) population derived from the cross of GH06 and P174 via single seed descent propagation was used for QTL mapping and candidate gene analysis. The fresh and dry weight of leaf and root were measured at 25 days after the seedlings were grown in Hoagland solution with 16 g L–1 NaCl. Composite interval mapping (CIM) was used to identify the related QTLs according to the high density SNP genetic map, and the candidate gene expression in the extreme lines tested by qRT-PCR. A total of 19 QTLs were identified in the control and salt stress treatment, and six QTLs were mapped on chromosomes A02, A04 and C03 under salt stress, with contribution rate ranged from 7.16% to 16.15%. Eight genes were identified according to the BLAST of genes in the QTL confidence intervals and the salt stress related genes in Arabidopsis. The expression of four candidate genes in the extreme lines showed that BnaA02g14680D and BnaA02g14490D under salt stress treatment for 48 or 72 hours had higher expression than the control, which indicates that the expressions are induced by salt stress. The relative expressions of gene BnaC03g64030D in sensitive extreme lines were higher than those in tolerant extreme lines. There were no changed in expression for gene BnaC03g62830D in sensitive extreme lines but increased expression at 48 hours and reduced expression at 72 hours after salt treatment in tolerant extreme lines, showing the enhance of plant salt tolerance possibly. Our research laid a foundation for the function research of salt tolerant gene in rapeseed and the breeding of salt tolerant rapeseed.

Key words: Brassica napus L, Salt stress, QTL, SNP, Candidate gene

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