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Acta Agron Sin ›› 2006, Vol. 32 ›› Issue (08): 1256-1260.

• RESEARCH NOTES • Previous Articles     Next Articles

Cloning and Expression Analysis of Maize LFY-like Gene

GAO Wei1,CHEN Yan-Hui1,KU Li-Xia1,REN Yong-Zhe1,CHANG Sheng-He1,WANG Tie-Gu1,CHEN Xiao1   

  1. 1Agronomy College, Henan Agricultural University, Zhengzhou 450002, Henan; 2Physics College, Zhengzhou University, Zhengzhou 450003, Henan
  • Received:2005-06-08 Revised:1900-01-01 Online:2006-08-12 Published:2006-08-12
  • Contact: CHEN Yan-Hui

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Abstract:

Two maize (Zea mays L. ) inbred lines, CML288 from tropical zone (CIMMYT) and Huangzao 4 from temperate zone (China) were employed in the study. Plants were grown in 2 different controlled photoperiod conditions with diurnal sunlight of 9h and 15 h, respectively. A 1 265 bp cDNA (LFY-like) clone was isolated from the stem top of maize by RT-PCR. The LFY-like gene shared a homology of 93% with zfl2, 84% with RFL, 57% with LFY and FLO. Compared to zfl1, the deduced protein lacks an alanine, proline, theonine in the position of 28th, 29th and 160th respectively and has an additional methionine in the position of 188th. Expression analysis showed the gene expressed all time under long-day condition, but not expressed at early or late stage of maize under short-day condition. The function of the gene needs to be studied further.

Key words: Maize, Photoperiod, LFY-like gene

CLC Number: 

  • S512
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