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Acta Agron Sin ›› 2007, Vol. 33 ›› Issue (03): 503-506.

• RESEARCH NOTES • Previous Articles     Next Articles

Genetic Diversity of Waxy Gene in Chinese Wheat Landraces

DU Xiao-Yan 1,2;HAO Chen-Yang1;ZHANG Xue-Yong 1,* ; MA Zheng-Qiang 2 ;YOU Guang-Xia1;WANG Lan-Fen1; DONG Yu-Shen 1   

  1. 1 Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture/ National Key Facility for Crop Gene Resources and Genetic Improvement/ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; 2 Applied Genomics Unit, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
  • Received:2006-04-12 Revised:1900-01-01 Online:2007-03-12 Published:2007-03-12
  • Contact: ZHANG Xue-Yong

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Abstract:

A total of 1 739 Chinese wheat landraces were screened for the Waxy gene mutations with two STS, one SSR and one gene specific marker published in previous researches. Among of the landraces, 3 were null Wx-A1 mutants, 25 were null Wx-B1 mutants, and 3 were null Wx-D1 mutants. Different null types of Waxy genes were also certified by SDS-PAGE of waxy proteins, containing that these markers were effective to verify mutant and normal alleles at waxy loci. These markers can also be used in marker-assisted selection in breeding program. Furthermore, based on our findings, we recommend some accessions with different Waxy mutations to breeders. Genotypes of Waxy genes of these materials were also released in this paper.

Key words: Waxy gene, Wheat, Landrace, Molecular marker, SDS-PAGE

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