Abstract:

The content of MDA, membrane permeability, SOD activity and developmental morpha of pollens during the pollen development in non-1B/1R and 1B/1R male sterile lines and their maintenance lines were analyzed. The results showed that the content of MDA and conductivity of pollen extract in two types of wheat sterile lines had nearly the same increasing tendency from meiophase to pollens diverging stage; whereas their maintenance lines had no remarkable difference between each other and these two indexes remained stable during the whole course of pollen development. SOD activity of both types of sterile lines showed a downtrend from uninucleate stage to trinucleate stage. The SOD activity was higher in both sterile lines than in their respective maintenance lines from uninucleate stage to binucleate stage; and that was lower in both sterile lines than in their respective maintenance lines at trinucleate stage, and that appeared in a continuous downtrend. Therefore, it could be inferred that the abortive processes and stages of the two sterile lines were almost the same; and that the key stages of pollen abortion were from uninucleate to trinucleate. However, from the cytalogical observation we found that pollen morpha was abnormal in non-1B/1R sterile lines at binucleate state but in 1B/1R sterile lines at trinucleate stage,indicating that pollen abortion of non-1B/1RK-type sterile lines was a little earlier than that of 1B/1RK-type sterile lines.

Key words: Wheat, Triticum.spelta 1BS, Non-1B/1R male sterile line, Pollen abortion