Abstract:

The common wheat (Triticum aestivum L.) Chuannong 18 with 1BL/1RS translocation chromosome, whose HMW-GS components is (1, 7+9, 2+12) as male and female parent respectively was crossed with Chuannong 19 (N, 7+8, 2+12). Both the female and male gametes of their F₂ progenies showed significantly (P<0.01) lower rate of 1BL/1RS translocation compared with the theoretical value (75%), in which female gamete (65.1%) had higher rate than male gamete (52.4%). In both crosses, HMW-GS subunits (encoded by Glu-A1 and Glu-B1) of parents were all transmitted to F₁ progenies as dominant trait, but deviated the Mendel’s segregation ratios at Glu-B1 and the combination of Glu-A1 & Glu-B1 loci in F₂ progenies. We also found and confirmed a variant seed in F₁ of Chuannong 19 × Chuannong 18 by detecting its genome DNA with 33 pairs of specific SSR markers and the components of HMW-GS and gliadin of its F₂ progeny. The HMW-GS patterns of the variant seed were (1, 7+8, x+9, 2+12). The band of variant subunit was similar to 1Bx6 by SDS-PAGE analysis, which was between 1Dx5 and 1Bx7.

Key words: Wheat, 1BL/1RS translocation, HMW-GS, Inheritance