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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (4): 597-601.doi: 10.3724/SP.J.1006.2009.00597

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Marker-assisted Selection for Stv-bi Gene Controlling Resistance to Rice Stripe Disease

CHEN Feng1,ZHOU Ji-Hua23,ZHANG Shi-Yong1,YAN Chang-Jie2,ZHU Wen-Yin1,SUN Ya-Wei2,YUAN Shou-Jiang1,YANG Lian-Qun1*   

  1. 1Shandong Rice Research Institute,Jining 272177,China;2Key Laboratory for Plant Functional Genomics,Ministry of education/Agricultural College,Yangzhou University,Yangzhou 225009,China;3Crop Breeding and Cultivation Reserch Institute, Shanghai Academy of Agricultural Sciences,Shanghai 201106,China
  • Received:2008-08-11 Revised:2008-12-08 Online:2009-04-12 Published:2009-02-13
  • Contact: YANG Lian-Qun E-mail:yangsdsd@sohu.com

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Abstract:

Rice stripe disease is one of the most serious viral diseases in Huang-Huai and Yangtze River japonica cultivating area in China, and has caused severe loss in rice production. The pathogen is rice stripe virus (RSV) and transmitted by the small brown plant-hopper, Laodelphax striatellus Fallen. Moreover, rice stripe disease is difficult to assess by the way of artificial inoculation, and easily affected by natural conditions, thereby marker-assisted selection using molecular markers closely linked to disease-resistant gene to improve the efficiency of resistance breeding programs is of great significance. At present, Stv-bi is a widely utilized resistant gene for stripe virus disease in rice breeding that came from indica variety Modan, and Stv-bi has been fine mapped on chromosome 11. In this study, eight molecular markers, including three SSR (Simple Sequence Repeat) and five STS (Sequence-tagged Sites), closely linked to Stv-bi, were developed and displayed polymorphic between Shengdao 13 and Zhendao 88. Among them, three markers, H21, H11-8 and H11-12 were subsequently used for marker-assisted selection. The individual seedlings of three compound breeding populations, F30718 (Shengdao 13/Zhendao 88), F50701 (Wuyou 34/T022//Shengdao 806), and F60702 (V6/T022//Zhendao 88) were genotyped with molecular markers H21, H11-8 and H11-12. The lines from these populations checked by marker-assisted selection to rice strip disease were also investigated under field conditions at next generation. The consistency between field performance and the marker genotype in the three compound breeding populations was 99.3%, 87.7%, and 91.8%, respectively. The results indicated that these molecular markers can be applied for marker-assisted selection in the improvement of resistance to RSV.

Key words: Rice, Stripe virus disease, Molecular marker, Breeding

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