Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (4): 597-601.doi: 10.3724/SP.J.1006.2009.00597

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Marker-assisted Selection for Stv-bi Gene Controlling Resistance to Rice Stripe Disease

CHEN Feng1,ZHOU Ji-Hua23,ZHANG Shi-Yong1,YAN Chang-Jie2,ZHU Wen-Yin1,SUN Ya-Wei2,YUAN Shou-Jiang1,YANG Lian-Qun1*   

  1. 1Shandong Rice Research Institute,Jining 272177,China;2Key Laboratory for Plant Functional Genomics,Ministry of education/Agricultural College,Yangzhou University,Yangzhou 225009,China;3Crop Breeding and Cultivation Reserch Institute, Shanghai Academy of Agricultural Sciences,Shanghai 201106,China
  • Received:2008-08-11 Revised:2008-12-08 Online:2009-04-12 Published:2009-02-13
  • Contact: YANG Lian-Qun E-mail:yangsdsd@sohu.com

PDF

1771

Cited

24

Abstract:

Rice stripe disease is one of the most serious viral diseases in Huang-Huai and Yangtze River japonica cultivating area in China, and has caused severe loss in rice production. The pathogen is rice stripe virus (RSV) and transmitted by the small brown plant-hopper, Laodelphax striatellus Fallen. Moreover, rice stripe disease is difficult to assess by the way of artificial inoculation, and easily affected by natural conditions, thereby marker-assisted selection using molecular markers closely linked to disease-resistant gene to improve the efficiency of resistance breeding programs is of great significance. At present, Stv-bi is a widely utilized resistant gene for stripe virus disease in rice breeding that came from indica variety Modan, and Stv-bi has been fine mapped on chromosome 11. In this study, eight molecular markers, including three SSR (Simple Sequence Repeat) and five STS (Sequence-tagged Sites), closely linked to Stv-bi, were developed and displayed polymorphic between Shengdao 13 and Zhendao 88. Among them, three markers, H21, H11-8 and H11-12 were subsequently used for marker-assisted selection. The individual seedlings of three compound breeding populations, F30718 (Shengdao 13/Zhendao 88), F50701 (Wuyou 34/T022//Shengdao 806), and F60702 (V6/T022//Zhendao 88) were genotyped with molecular markers H21, H11-8 and H11-12. The lines from these populations checked by marker-assisted selection to rice strip disease were also investigated under field conditions at next generation. The consistency between field performance and the marker genotype in the three compound breeding populations was 99.3%, 87.7%, and 91.8%, respectively. The results indicated that these molecular markers can be applied for marker-assisted selection in the improvement of resistance to RSV.

Key words: Rice, Stripe virus disease, Molecular marker, Breeding

[1] Wang C-L(王才林). Advances in breeding of rice with resistance to rice stripe disease in Jiangsu, China. Jiangsu Agric Sci (江苏农业科学), 2006, (3): 1–5(in Chinese)
[2] Gao L-C(高苓昌), Song K-Q(宋克勤), Zhang H-R(张洪瑞), Du B-H(杜本怀), Zhu Q-S(朱其松). Pathogeny feature and integrate control of rice strip blight in Huang-Huai rice area. Shangdong Agric Sci (山东农业科学), 2006, (3): 66–67(in Chinese)
[3] Wang C-L(王才林), Zhang Y-D(张亚东), Zhu Z(朱镇), Zhao L(赵凌), Chen T(陈涛). Rice breeding for resistance to stripe virus disease. Acta Agron Sin (作物学报), 2008, 34(3): 530–533(in Chinese with English abstract)
[4] Pan X-B(潘学彪), Liang G-H(梁国华), Chen Z-X(陈宗祥), Zhang Y-F(张亚芳). Breeding strategy on resistance to rice stripe in Jiangsu. Jiangsu Agric Sci (江苏农业科学), 2005, (5): 22–23(in Chinese)
[5] Hayano-Saito Y, Tsuji T, Fujii K, Saito K, Iwasaki M, Saito A. Localization of the rice stripe disease resistance gene, Stv-bi, by graphical genotyping and linkage analyses with molecular markers. Theor Appl Genet, 1998, 96: 1044–1049
[6] Hayano-Saito Y, Saito K, Nakamura S. Kawasaki S, Iwasaki M. Fine physical mapping of the rice stripe resistance gene locus, Stvb-i. Theor Appl Genet, 2000, 101: 59–63
[7] Wu S J, Zhong H, Zhou Y, Zuo H, Zhou L H, Zhu J Y, Ji C Q, Gu S L, Gu M H, Liang G H. Identification of QTLs for the resistance to rice stripe virus in the indica rice variety Dular. Euphytica, 2009, 165: 557–565
[8] Wang C-F(王春芳), Yan C-J(严长杰), Liang G-H(梁国华), Dong X-S(董学锁), Yu H-X(于恒秀), Gu M-H(顾铭洪). Construction and characteristic analysis of rice clonally propagated F2 population. J Yangzhou Univ (Agric＆Life Sci Edn) (扬州大学学报·农业与生命科学版), 2002, 23(2): 41–45(in Chinese with English abstract)
[9] Ding X-L (丁秀兰), Jiang L(江玲), Liu S-J(刘世家), Wang C-M(王春明), Chen L-M(陈亮明), Cheng Z-B(程兆榜) , Fan Y-J(范永坚), Zhou Y-J(周益军), Wan J-M(万建民) . QTL analysis for rice stripe disease resistance gene using recombinant inbred lines (RILs) derived from crossing of Kin maze and DV85. Acta Genet Sin (遗传学报), 2004, 31(3): 287–292(in Chinese with English abstract)
[10] Ding X-L(丁秀兰), Jiang L(江玲), Zhang Y-X(张迎信), Sun D-Z(孙黛珍), Zhai H-Q(翟虎渠), Wan J-M(万建民). Detection and analysis of QTL for resistance to stripe disease in rice , using backcross inbred lines, Acta Agron Sin (作物学报), 2005, 38(5): 1041–1046(in Chinese with English abstract)
[11] Maeda H, Sugisawa T, Nemoto H, Sunohara Y. QTL analysis for rice stripe resistance in the Japanese upland rice kanto 72, Breed Sci, 2004, 54: 19–26
[12] Gong D-Y(宫德英), Chen F(陈峰), Zhang S-Y(张士永), Yuan S-J(袁守江), Yang L-Q(杨连群), Sang M-P(桑茂鹏). Breeding and cultivation technology of a new rice cultivar Shengdao 13. Shangdong Agric Sci (山东农业科学), 2007, (5):113–114(in Chinese)
[13] Washio O, Ezuka A, Toriyama K, Sakurai Y. Testing method for genetics and breeding for resistance to rice stripe disease. Bull Chugoku Agric Exp Stn, 1968, 16: 39–197
[14] Sugiura N, Tsuji T, Fujii K, Kato T, Saka N, Touyama T, Hayano-Saito Y, Izawa T. Molecular marker-assisted selection in a recurrent backcross breeding for the incorporation of resistance to rice stripe virus and panicle blast in rice (Oryza sativa L.). Breed Res, 2004, 6: 143–148(in Japanese with English abstract)
[15] Hayano-Saito Y, Saito K, Fujii K, Touyama T, Tsuji T, Sugiura N, Izawa T, Iwasaki M. SCAR marker for selection of the rice stripe resistance gene Stvb-i. J Breed Res, 2000, 2: 67–72 (in Japanese with English abstract)
[16] Sun L-J (孙林静), Ma Z-Y(马忠友), Su J-P(苏京平), Liu X-J(刘学军), Wang C-M(王春敏), Wang S-J(王胜军), Yan S-Y(闫双勇). Detection of the rice stripe disease resistance gene Stvb-i by molecular marker. Tianjin Agric Sci (天津农业科学), 2007, 13(3): 9–11(in Chinese with English abstract)
[17] Sun D-Z(孙黛珍), Jiang L(江玲), Zhang Y-X(张迎信), Cheng X-N(程遐年), Zhai H-Q(翟虎渠), Wan J-M(万建民). Detection of QTL associated with rice stripe resistance in cultivar IR24. Acta Agron Sin (作物学报), 2007, 33(1): 25–30(in Chinese with English abstract)
[1] TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[2] ZHENG Chong-Ke, ZHOU Guan-Hua, NIU Shu-Lin, HE Ya-Nan, SUN wei, XIE Xian-Zhi. Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1389-1400.
[3] ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[4] ZHENG Xiao-Long, ZHOU Jing-Qing, BAI Yang, SHAO Ya-Fang, ZHANG Lin-Ping, HU Pei-Song, WEI Xiang-Jin. Difference and molecular mechanism of soluble sugar metabolism and quality of different rice panicle in japonica rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1425-1436.
[5] YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[6] YANG Jian-Chang, LI Chao-Qing, JIANG Yi. Contents and compositions of amino acids in rice grains and their regulation: a review [J]. Acta Agronomica Sinica, 2022, 48(5): 1037-1050.
[7] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[8] YANG De-Wei, WANG Xun, ZHENG Xing-Xing, XIANG Xin-Quan, CUI Hai-Tao, LI Sheng-Ping, TANG Ding-Zhong. Functional studies of rice blast resistance related gene OsSAMS1 [J]. Acta Agronomica Sinica, 2022, 48(5): 1119-1128.
[9] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[10] WANG Xiao-Lei, LI Wei-Xing, OU-YANG Lin-Juan, XU Jie, CHEN Xiao-Rong, BIAN Jian-Min, HU Li-Fang, PENG Xiao-Song, HE Xiao-Peng, FU Jun-Ru, ZHOU Da-Hu, HE Hao-Hua, SUN Xiao-Tang, ZHU Chang-Lan. QTL mapping for plant architecture in rice based on chromosome segment substitution lines [J]. Acta Agronomica Sinica, 2022, 48(5): 1141-1151.
[11] WANG Ze, ZHOU Qin-Yang, LIU Cong, MU Yue, GUO Wei, DING Yan-Feng, NINOMIYA Seishi. Estimation and evaluation of paddy rice canopy characteristics based on images from UAV and ground camera [J]. Acta Agronomica Sinica, 2022, 48(5): 1248-1261.
[12] KE Jian, CHEN Ting-Ting, WU Zhou, ZHU Tie-Zhong, SUN Jie, HE Hai-Bing, YOU Cui-Cui, ZHU De-Quan, WU Li-Quan. Suitable varieties and high-yielding population characteristics of late season rice in the northern margin area of double-cropping rice along the Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(4): 1005-1016.
[13] CHEN Yue, SUN Ming-Zhe, JIA Bo-Wei, LENG Yue, SUN Xiao-Li. Research progress regarding the function and mechanism of rice AP2/ERF transcription factor in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 781-790.
[14] WANG Lyu, CUI Yue-Zhen, WU Yu-Hong, HAO Xing-Shun, ZHANG Chun-Hui, WANG Jun-Yi, LIU Yi-Xin, LI Xiao-Gang, QIN Yu-Hang. Effects of rice stalks mulching combined with green manure (Astragalus smicus L.) incorporated into soil and reducing nitrogen fertilizer rate on rice yield and soil fertility [J]. Acta Agronomica Sinica, 2022, 48(4): 952-961.
[15] QIN Qin, TAO You-Feng, HUANG Bang-Chao, LI Hui, GAO Yun-Tian, ZHONG Xiao-Yuan, ZHOU Zhong-Lin, ZHU Li, LEI Xiao-Long, FENG Sheng-Qiang, WANG Xu, REN Wan-Jun. Characteristics of panicle stem growth and flowering period of the parents of hybrid rice in machine-transplanted seed production [J]. Acta Agronomica Sinica, 2022, 48(4): 988-1004.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd