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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (4): 588-596.doi: 10.3724/SP.J.1006.2009.00588

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

MSAP Analysis of Epigenetic Changes in Cotton(Gossypium hirsutum L.) under Salt Stress

LI Xue-Lin12,LIN Zhong-Xu1,NIE Yi-Chun1,GUO Xiao-Ping1,ZHANG Xian-Long1*   

  1. Huazhong Agricultural University, Wuhan 430070,China;2College of Agronomy,Henan University of Science and Technology,Luoyang 471003,China
  • Received:2008-10-12 Revised:2009-01-10 Online:2009-04-12 Published:2009-02-13
  • Contact: ZHANG Xian-Long E-mail:xlzhang@mail.hzau.edu.cn E-mail:xlzhang@mail.hzau.edu.cn

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Abstract:

Salinity is one of the important limiting factors in plant production worldwide. The objectives of the study were to assess the effect of salt stress on the plant growth and to determine if DNA can be methylated in cotton plants (Gossypium hirsutum) by methylation-sensitive amplified polymorphism (MSAP) technique. The results showed that 100 mmol L-1 NaCl obviously promoted plant height and root length of cotton seedlings, but 200 mmol L-1 NaCl significantly inhibited plant growth; 100–200 mmol L-1 NaCl inhibited the number of lateral root considerably. The analysis of MSAP showed that the level of global DNA methylation decreased from 41.2% to 34.5% as the salt concentrations increased; there was a significantly negative correlation (r = –0.986) between NaCl concentrations and the level of DNA methylation in cotton roots. Under stresses of 100, 150 and 200 mmol L-1 NaCl, methylation and demethylation of DNA were 6.4%, 7.6%, 11.3% and 12.7%, 11.1%, 8.2%, respectively. In addition, the analyses of sequences and RT-PCR showed that expressions of genes homologous to MSAP fragments in roots were different between control and treated plants under salt stress, suggesting that these genes would play an important role in the cotton adaptation of salt stress.

Key words: Cotton, Salt stress, DNA methylation, MSAP, RT-PCR

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