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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (8): 1451-1457.doi: 10.3724/SP.J.1006.2009.01451

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Construction of  Fingerprinting and Analysis of Genetic Diversity with SSR Markers for Eighty-Eight Approved Potato Cultivars(Solanum tuberosum L.)in China

DUAN Yan-Feng,LIU Jie,BIAN Chun-Song,DUAN Shao-Guang,XU Jian-Fei,JIN Li-Ping*   

  1. Institute of Vegetables and Flowers of Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2008-12-29 Revised:2009-04-25 Online:2009-08-12 Published:2009-06-10
  • Contact: JIN Li-Ping,Tel: 010-82109543
  • About author:E-mail: duanyanfeng1110@163.com; Tel: 13810761380

Abstract:

Potato (Solanum tuberosum L.) is one of the most important food crops in the world. Approved potato cultivars have contributed a lot not only to potato production but also to varietal improvement as germplasm resource, and about 110 potato cultivars were approved during 2000–2007 in China. It is necessary to make potato cultivar identification and genetic relationship analysis for seed production, germplasm management, plant variety protection and breeding practice. Currently, the simple sequence repeats (SSRs) are preferred as molecular markers due to their highly desirable properties. In this study, for the aim of cultivar identification and parents combination at the molecular level, SSR markers were employed to analysis on fingerprinting and genetic diversity of 88 potato cultivars approved in China during 2000–2007. Ten of one hundred and thirty-eight pairs of SSR primers were screened out based on sixteen accessions distinct in genetics. The 10 primer pairs amplified a total of 135 alleles (including 133 polymorphic alleles) among the 88 cultivars, and the ratio of polymorphism was as high as 98.52%.Alleles amplified by each pair of primers ranged from 7 (primer S7) to 22 (primer S189), with a mean of 13.5. The polymorphic information content values (PIC) ranged from 0.7604 (primer S192) to 0.9375 (primer S189), with a mean of 0.8501. The fragment sizes varied from 80 to 380 bp. The fingerprinting of 88 cultivars was constructed by 6 pairs of primers of S180, S25, S7, S151, S184, and S192. Eighty-seven out of eighty-eight cultivars were univocally identified by using only five SSR primers (S180, S25, S7, S151, and S184). UPGMA cluster analysis of genetic similarity showed that all the materials were clustered in to one group at the genetic similarity of 0.620, and 81.8% of the cultivars were still clustered together at the genetic similarity of 0.652.The genetic relationships of cultivars were identical to the family tree basically. It’s indicated that the genetic basis of potato cultivars in China is narrow, and the genetic relationship of the potato cultivars derived from the same district is similar. Thefingerprinting and analysis of genetic diversity in this studygave a basis for exploration and utilization of approved potato cultivars as germplasm resources.

Key words: Potato, Approved cultivars, SSR markers, DNA fingerprinting, Genetic diversity



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