Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (07): 1216-1220.doi: 10.3724/SP.J.1006.2010.01216

• RESEARCH NOTES • Previous Articles     Next Articles

FISH Analysis of Resynthesized Allotetraploid Arabidopsis

LI Fang,ZHANG Gai-Sheng   

  1. Key Laboratory of Crop Heterosis of Shaanxi Province, Wheat Breeding Engineering Research Center, Ministry of Education, Northwest A&F University, Yangling 712100, China
  • Received:2009-10-26 Revised:2010-04-21 Online:2010-07-12 Published:2010-05-20
  • Contact: ZHANG Gai-Sheng, E-mail: zhanggsh@public.xa.sn.cn

PDF

1511

Abstract:

Allopolyploidy breeding or introduction of preferential gene(s) via allopolyploidization is widely attempted in production of new crops. Thus at the onset of establishment of new allopolyploids, the functional gametes are reproduced conditioning that normal events such as synapsis and pairing and correct segregation of homologous chromosomes are guaranteed with the avoidance of possible interference of homoeologous chromosomes during meiosis. In the present study, we reported on meiotic synapsis and pairing of homologous chromosomes during the development of pollen mother cells in the newly resynthesized allotetraploid Arabidopsis aided with DAPI staining and FISH technique. The results showed that the correct number of nuclear chromosomes and balanced segregation were frequently observed with DAPI staining, and FISH analysis further provided the improved resolution of synapsis and pairing of homologous chromosomes, and the investigated nuclear chromosomes derived from different parental lines had no interference with each other, and the synapsis and pairing of homologous chromosomes were clearly identified. Therefore, the results indicated that the newly resynthesized allotetraploid Arabidopsis may achieve the normal meiosis and propagate the functionalized gametes for fertilization, confirming a potential vigor of intra/interspecific hybridization and a cytological basis for allopolyploid breeding.

Key words: Arabidopsis, Allotertraploid, Resynthesized, DAPI, FISH


[1] Levsky J M, Singer R H. Fluorescence in situ hybridization: past, present and future. J Cell Sci, 2003, 116: 2833–2838


[2] Jiang J M, Gill B S. Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research. Genome, 2006, 49: 1057–1068


[3] Gorman P, Roylance R. Fluorescence in situ hybridization and comparative genomic hybridization. Methods Mol Med, 2006, 120: 269–295


[4] Ali H B, Lysak M A, Schubert I. Genomic in situ hybridization in plants with small genomes is feasible and elucidates the chromosomal parentage in interspecific Arabidopsis hybrids. Genome, 2004, 47: 954–960


[5] Doyle J J, Flagel L E, Paterson A H, Rapp R A, Soltis D E, Soltis P S, Wendel J F. Evolutionary genetics of genome merger and doubling in plants. Annu Rev Genet, 2008, 42: 443–461


[6] Comai L, Tyagi A P, Winter K, Holmes-Davis R, Reynolds S H, Stevens Y, Byers B. Phenotypic instability and rapid gene silencing in newly formed Arabidopsis allotetraploids. Plant Cell, 2000, 12: 1551–1568


[7] Schranz M E, Osborn T C. Novel flowering time variation in the resynthesized polyploid Brassica napus. J Hered, 2000, 91: 242–246


[8] Madlung A, Tyagi A P, Watson B, Jiang H, Kagochi T, Doerge R W, Martienssen R, Comai L. Genomic changes in synthetic Arabidopsis polyploids. Plant J, 2005, 41: 221–230


[9] Song K, Lu P, Tang K, Osborn T C. Rapid genome change in synthetic polyploids of Brassica and its implications for polyploid evolution. Proc Natl Acad Sci USA, 1995, 92: 7719–7723


[10] Pikaard C S. Genomic change and gene silencing in polyploids. Trends Genet, 2001, 17: 675–677


[11] Chen Z J. Genetic and epigenetic mechanisms for gene expression and phenotypic variation in plant polyploids. Annu Rev Plant Biol, 2007, 58: 377–406


[12] Liu B, Wendel J F. Epigenetic phenomena and the evolution of plant allopolyploids. Mol Phylogenet Evol, 2003, 29: 365–379


[13] Roberts M A, Reader S M, Dalgliesh C, Miller T E, Foote T N, Fish L J, Snape J W, Moore G. Induction and characterization of Ph1 wheat mutants. Genetics, 1999, 153: 1909–1918


[14] Griffiths S, Sharp R, Foote T N, Bertin I, Wanous M, Reader S, Colas I, Moore G. Molecular characterization of Ph1 as a major chromosome pairing locus in polyploid wheat. Nature, 2006, 439: 749–752


[15] Comai L, Madlung A, Josefsson C, Tyagi A. Do the different parental 'heteromes' cause genomic shock in newly formed allopolyploids. Philos Trans R Soc Lond B Biol Sci, 2003, 358: 1149–1155


[16] Wang A-Y(王爱云), Chen D-L(陈冬玲), Cai D-T(蔡得田). Applications of wide hybridization and allopolyploidization in Rice Breeding. J Wuhan Bot Res (武汉植物学研究), 2005, 23(5) : 491–4954 (in Chinese with English abstract)


[17] Stewart C N Jr, Via L E. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. Biotechniques, 1993, 14: 748–50


[18] Ross K J, Fransz P, Jones G H. A light microscopic atlas of meiosis in Arabidopsis thaliana. Chromosome Res, 1996, 4: 507–516


[19] Armstrong S J, Sanchez-Moran E, Franklin F C. Cytological analysis of Arabidopsis thaliana meiotic chromosomes. Methods Mol Biol, 2009, 558: 131–145


[20] Comai L. The advantages and disadvantages of being polyploid. Nat Rev Genet, 2005, 6: 836–846


[21] Zhuang Y(庄勇), Chen L-Z(陈龙正), Yang Y-G(杨寅桂), Lou Q-F(娄群峰), Chen J-F(陈劲枫). Changes in gene expression in evolution of plant allopolyploids. Chin Bull Bot (植物学通报), 2006, 23(2): 207–214 (in Chinese with English abstract)


[22] Xiong Z-Y(熊志勇), Gao Y(高原), He G-Y(何光源), Gu M-G(谷明光), Guo L-Q(郭乐群), Song Y-C(宋运淳). Distribution of the knob heterochromatin repeat sequence on chromosome in maize, perennial diploid maize and their offspring. Chin Sci Bull (科学通报), 2004, 12(49): 1162–1165 (in Chinese with English abstract)


[23] Wang J L, Tian L, Lee H S, Wei N E, Jiang H M, Brian W, Andreas M, Osborn T C, Doerge R W, Comai L, Chen Z J. Genomewide nonadditive gene regulation in Arabidopsis allotetraploids. Genetics, 2006, 172: 507–517


[24] Beaulieu J, Jean M, Belzile F. The allotetraploid Arabidopsis thaliana-Arabidopsis lyrata subsp. petraea as an alternative model system for the study of polyploidy in plants. Mol Genet Genom, 2009, 281: 421–435


[25] Moore G. Meiosis in allopolyploids—the importance of ‘Teflon’ chromosomes. Trends Genet, 2002, 18: 456–463
[1] TAO Jun, LAN Xiu-Jin. Molecular cytogenetic identification of wheat-Thinopyrum intermedium 2A/6St substitution strain 014-459 [J]. Acta Agronomica Sinica, 2022, 48(2): 511-517.
[2] Yi YUAN,Shuang ZHU,Ting-Ting FANG,Jin-Jin JIANG,You-Ping WANG. Analysis of drought resistance and DNA methylation level of resynthesized Brassica napus [J]. Acta Agronomica Sinica, 2019, 45(5): 693-704.
[3] LI Li-Na,,DU Pei,FU Liu-Yang,LIU Hua,XU Jing,QIN Li,YAN Mei,HAN Suo-Yi,HUANG Bing-Yan,DONG Wen-Zhao,TANG Feng-Shou,ZHANG Xin-You. Development and Characterization of Amphidiploid Derived from Interspecific Cross between Cultivated Peanut and Its Wild Relative Arachis oteroi [J]. Acta Agron Sin, 2017, 43(01): 133-140.
[4] XIE Tao,RONG Hao,JIANG Jin-Jin*,KONG Yue-Qin,RAN Li-Ping,WU Jian,WANG You-Ping. Analysis of DNA Methylation Patterns in Resynthesized Brassica napus and Diploid Parents [J]. Acta Agron Sin, 2016, 42(04): 513-524.
[5] Lü Yan-Yan,FU San-Xiong,CHEN Song,ZHANG Wei,QI Cun-Kou*. Cloning of BnADH3 Gene from Brassica napus L. and Submergence Tolerance of BnADH3 Transgenic Arabidopsis [J]. Acta Agron Sin, 2015, 41(04): 565-573.
[6] LUO Qiao-Ling,ZHENG Qi,XU Yun-Feng,LI Li-Hui,HAN Fang-Pu,XU Hong-Xing,LI Bin,MA Peng-Tao,AN Diao-Guo. Main Agronomic Traits of 390 Wheat-Rye Derivatives and GISH/FISH Identification of Their Outstanding Materials [J]. Acta Agron Sin, 2014, 40(08): 1331-1339.
[7] LIU Jin-Shuai, LAI Hui-Cheng, JIA Zhen-Hong. Image Segmentation of Cotton Based on YCbCcr Color Space and Fisher Discrimination Analysis [J]. Acta Agron Sin, 2011, 37(07): 1274-1279.
[8] GAO Shi-Qing, CHEN Ming, XU Zhao-Shi, TANG Yi-Miao, LI Lian-Cheng, MA Wei-Zhi, ZHAO Chang-Ping. GmAREB Gene Improves Tolerances to Drought and Oxidation in Transgenic Arabidopsis [J]. Acta Agron Sin, 2011, 37(06): 982-990.
[9] PENG Ren-Hai;SONG Guo-Li;LIU Fang;LI Shao-Hui;WANG Chun-Ying;ZHANG Xiang-Di;WANG Yu-Hong;WANG Kun-Bo. An Efficient Method of Cotton DNA Fibers Preparation for FISH [J]. Acta Agron Sin, 2009, 35(3): 412-417.
[10] KONG Fang;JIANG Jin-Jin;WU Lei;WANG You-Ping. Relationships among Genome A, B, and C Revealed by FISH and CAPS [J]. Acta Agron Sin, 2008, 34(07): 1188-1192.
[11] GUO Dong-Wei;HU Gan;SHE Mao-Yun;LI Lian-Cheng;CHEN Ming;XU Zhao-Shi;MA You-Zhi
. Identification of Wheat Chromosomes Sorted by Flow Cytometry [J]. Acta Agron Sin, 2008, 34(01): 89-94.
[12] LAN Wei-Zhen;LIU Zhe-Sheng;LI Gang;QIN Rui. Comparative Physical Mapping of Bph15 with BAC-FISH in O. glaberrima, O. officinalis, and O. latifolia [J]. Acta Agron Sin, 2007, 33(04): 560-565.
[13] LIU Ping-Wu;YANG Guang-Sheng. Analyses of the Genetic Diversity of Resynthesized Brassica napus by RAPD and SSR Molecular Markers [J]. Acta Agron Sin, 2004, 30(12): 1266-1273.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd