大豆脂肪酸含量的QTL分析

doi:10.3724/SP.J.1006.2010.01498

Abstract

Abstract: Soybean, Glycine max (L.) Merr., is one of the main plant oil sources. Most researches have focused on the improvement of oil content. However, improving fatty acid composition is the main aspect of soybean breeding now. This study aimed at mapping QTLs conferring fatty acids contents in soybean on the genetic map which had been constructed in our lab to provide a reference of soybean oil quality breeding. Five kinds of fatty acid contents of 154 F₁₄ and F₁₅ lines devisedqSt-B1-1, qSt-B1-2, qSt-D1a-1 and qSt-C2-1;three for related with oleic acid content, i.e. qOle-B2-1, qOle-G-1, and qOle-H-1; two for related with linoleic acid content, i.e. qLin-C2-1 and qLin-H-1, and four for linolenic acid content, i.e. qLino-B1-1,qLino-C2-1, qLino-D1b-1, and qLino-J-1. These stable QTLs pave a way for specific fatty acid contents breeding by marker-assisted selection. The number of main-effect QTLs with large effect conferring the contents of fatty acids appeared not as many as expected, it may also subject to a number of the micro-effect genes which failed to detect. from a cross of Charleston (♀) × Dongnong 594 (♂) were measured by GC at three locations in two years. The QTLs of fatty acids contents were analyzed with method of composite interval mapping (CIM) by Win QTL Cartographer 2.5. In total, 47 related QTLs distributed on the 13 linkage groups were obtained. Seventeen QTLs were detected in different years or different sites, including two for palmitic acid content, i.e. qPal-C2-2 and qPal-A1-1; four for stearic acid content, i.e.

Key words: Soybean, Fatty acids contents, QTL analysis

MIAO Xin-Fen, ZHU Ming-Xi, XU Wen-Ping, DING Jun-Jie, YU Feng-Yao, YU Yong-Mei, DU Sheng-Wei, LIU Chun-Yan, CHEN Qiang-Shan, HU Guo-Hua. QTL Analysis of Fatty acids Contents in Soybean[J].Acta Agron Sin, 2010, 36(09): 1498-1505.

References

[1] Cregan P B, Jarvik T, Bush A L, Shoemaker R C, Lark K G, Kahler A L, VanToai T T, Lohnes D O, Chung J, Specht J E. An integrated genetic linkage map of the soybean genome. Crop Sci, 1999, 39: 1464–1490
[2] Song Q J, Marek L F, Shoemaker R C, Lark K G, Concibido V C, Delannay X, Specht J E, Cregan P B. A new integrated genetic linkage map of the soybean. Theor Appl Genet, 2004, 109: 122–128
[3] Li Z L, Wilson R F, Rayford W E, Boerma H R. Molecular mapping genes conditioning reduced palmitic acid content in N87-2122-4 soybean. Crop Sci, 2002, 42: 373–378
[4] Spencer M M, Pantalone V R, Meyer E J. Mapping the Fas locus controlling stearic acid content in soybean. Theor Appl Genet, 2003, 106: 615–619
[5] Hyten D L, Pantalone V R, Sams C E, Saxton A M, Landau-Ellis D, StefaniakT R, Schmidt M E. Seed quality QTL in a prominent soybean population. Theor Appl Genet, 2004, 109: 552–561
[6] Orf J H, Diers B W, Boerma H R. Genetic improvement: conventional and molecular based strategies. In: Soybeans: Improvement, Production, and Uses, 3rd edn. American Society of Agronomy. Inc. USA, 2004. pp 417–450
[7] Panthee D R, Pantalone V R, West D R, Saxton A M, Sams C E. Quantitative trait loci for seed protein and oil concentration, and seed size in soybean. Crop Sci, 2005, 45: 2015–2022
[8] Zheng Y-Z(郑永战), Gai J-Y(盖钧镒), Lu W-G(卢为国), Li W-D(李卫东), Zhong R-B(周瑞宝), Tian S-J(田少君). QTL mapping for fat and fatty acid composition contents in soybean. Acta Agron Sin (作物学报), 2006, (12): 1823–1830 (in Chinese with English abstract)
[9] Lu W-G(卢为国), Gai J-Y(盖钧镒), Zheng Y-Z(郑永战), Li W-D(李卫东). Construction of a genetic linkage map of soybean and mapping QTLs resistant to soybean cyst nematode (Heterodera glycines Ichinohe). Acta Agron Sin (作物学报), 2006, 32(9): 1272–1279 (in Chinese with English abstract)
[10] Shan D-P(单大鹏), Qi Z-M(齐照明), Qiu H-M(邱红梅), Hu G-H(胡国华), Chen Q-S(陈庆山). Epistatic effects of QTLs and QE interaction effects on oil content in soybean. Acta Agron Sin (作物学报), 2008, 34(6): 952–957 (in Chinese with English abstract)
[11] Chen Q-S(陈庆山), Zhang Z-C(张忠臣), Liu C-Y(刘春燕). Construction and analysis of soybean genetic map using recombinant inbred line of Charleston×Dongnong 594. Sci Agric Sin (中国农业科学), 2005, 38(7): 1312–1316 (in Chinese with English abstract)
[12] Wang D-L(王道龙), Zhu J(朱军), Li Z-K(黎志康), Paterson A H. QTLMaper1.6.(2004-12)
[2005-2]. http://ibi.zju.edu.cn/ software/qtlmapper/index.htm
[13] Wang D-L(王道龙), Zhu J(朱军), Li Z-K(黎志康),Paterson A H. Mapping QTLs with epistatic effects and QTL-environment interactions by mixed linear model approaches. Theor Appl Genet, 1999, 99: 1255–1264
[14] McCouch S R, Chen X, Panaud O. Microsatellite markers development mapping and applications in rice genetics and breeding. Plant Mol. Bio, 1997, 35: 89–99
[15] Kang M(康明). Analysis on genetic and molecular marker of oleic acid and linolenic acid in soybean. MS Dissertation of Northeast Agricultural University. 2008 (in Chinese with English abstract)
[16] Yang L(杨柳), Zhang B-B(张彬彬), Han Y-P(韩英鹏), Li W-B(李文滨). QTL analysis of linolenic acid content in soybean. Soybean Sci (大豆科学), 2006, 27(3): 270–273 (in Chinese with English abstract)
[17] Wang X-Y(王晓燕), Zhang C-Y(张彩英), Jia X-Y(贾晓艳). Analysis of fatty acids composition and content in soybean varieties in Hebei province. J Agric Univ Hebei (河北农业大学学报), 2007, 30(2): 15–18 (in Chinese with English abstract) Zhang Y-J(张颖君), Gao H-M(高慧敏), Jiang C-Z(蒋春志). Fast analysis on fatty acids of soybean seed by gas chromatograph. Soybean Sci (大豆科学), 2008, 27(5): 859–862 (in Chinese with English abstract)

Related Articles 15

[1]	Tang Kuan-Qiang, Li Gong-Yun, Song Mei-Yi, Zhao Xue, Chang Chun-Ling. Genome-wide association analysis and prediction model construction for soybean plant height [J]. Acta Agronomica Sinica, 2026, 52(6): 1743-1756.
[2]	Yao Shu, Guo Kai-Yue, Zhai Hui-Hui, Yao Jia-Hui, Deng Wen-Qi, Yan Ling, Huang Chi, Gao Yang, Yu Yan-Ran, Zhao Zhen-Bang, Li Ying-Hui, Wang Xiao-Bo, Li Jia-Jia. Comprehensive evaluation of low-iron tolerance and screening of elite germplasm at the soybean seedling stage [J]. Acta Agronomica Sinica, 2026, 52(5): 1373-1387.
[3]	Zhang Qing, Yang Yu, Guo Qian, Yue Pei-Yao, Yin Cong-Cong, Niu Jing-Ping, Zhao Jin-Zhong, Du Wei-Jun, Yue Ai-Qin. Cloning and functional analysis of the soybean GmARA6a gene in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(2): 480-493.
[4]	WANG Ke-Jing, LI Xiang-Hua. Endangerment assessment of the perennial species G. tabacina and G. tomentella of the genus Glycine Willd. in China [J]. Acta Agronomica Sinica, 2025, 51(8): 2009-2019.
[5]	MENG Ran, LI Zhao-Jia, FENG Wei, CHEN Yue, LIU Lu-Ping, YANG Chun-Yan, LU Xue-Lin, WANG Xiu-Ping. Comprehensive evaluation of salt tolerance at different growth stages of soybean and screening of salt-tolerant germplasm [J]. Acta Agronomica Sinica, 2025, 51(8): 1991-2008.
[6]	HE Hong-Li, ZHANG Yu-Han, YANG Jing, CHENG Yun-Qing, ZHAO Yang, LI Xing-Nuo, SI Hong-Liang, ZHANG Xing-Zheng, YANG Xiang-Dong. Creation and physiological analysis of an e1-as gene mutant in soybean [J]. Acta Agronomica Sinica, 2025, 51(8): 2228-2239.
[7]	HU Meng, SHA Dan, ZHANG Sheng-Rui, GU Yong-Zhe, ZHANG Shi-Bi, LI Jing, SUN Jun-Ming, QIU Li-Juan, LI Bin. QTL mapping and candidate gene screening for branch number in soybean [J]. Acta Agronomica Sinica, 2025, 51(7): 1747-1756.
[8]	WANG Qiong, ZOU Dan-Xia, CHEN Xing-Yun, ZHANG Wei, ZHANG Hong-Mei, LIU Xiao-Qing, JIA Qian-Ru, WEI Li-Bin, CUI Xiao-Yan, CHEN Xin, WANG Xue-Jun, CHEN Hua-Tao. Genome-wide association analysis and candidate genes prediction of flowering time and maturity date traits in soybean (Glycine max L.) [J]. Acta Agronomica Sinica, 2025, 51(6): 1558-1568.
[9]	YIN Cong-Cong, LI Rui-Qi, YUE Pei-Yao, LI Chen, NIU Jing-Ping, ZHAO Jin-Zhong, DU Wei-Jun, YUE Ai-Qin. Establishment and application of a visual detection method for soybean mosaic virus SC15 based on closed dumbbell mediated isothermal amplification [J]. Acta Agronomica Sinica, 2025, 51(5): 1248-1260.
[10]	XU Rui, HE Miao-Hua, WANG Hao, LI Wei, REN Jie, XIA Zhi-Qiang. Spatial transcriptomic analysis of soybean embryonic responses to X-ray irradiation [J]. Acta Agronomica Sinica, 2025, 51(12): 3121-3132.
[11]	LIN Yang, SHI Xiao-Lei, CHEN Qiang, LIU Bing-Qiang, YANG Qing, YU Hui-Juan, YAN Long, WU Xiao-Xia, YANG Chun-Yan. QTL mapping of soybean protein, oil, and fatty acid components [J]. Acta Agronomica Sinica, 2025, 51(11): 2899-2910.
[12]	LI Wei, ZHU Yu-Peng, SUN Bin-Cheng, WEN You-Xiang, WU Zong-Sheng, XU Yi-Fan, SONG Wen-Wen, XU Cai-Long, WU Cun-Xiang. Transgenic soybean combined with no-tillage flat planting promotes the simplification of soybean production in Northeast China [J]. Acta Agronomica Sinica, 2025, 51(10): 2738-2749.
[13]	CHEN Min, JIA Rong, ZHANG Jin-Chuan, ZHANG Chen-Yu, CHU Jun-Cong, YAO Wei, GE Jun-Yong, WANG Xing-Yu, YANG Ya-Dong, ZENG Zhao-Hai, ZANG Hua-Dong. Yield advantages and nitrogen utilization characteristics of oat and legume strip intercropping in semi-arid zones [J]. Acta Agronomica Sinica, 2025, 51(10): 2727-2737.
[14]	QIAN Yu-Ping, SU Bing-Bing, GAO Ji-Xing, RUAN Fen-Hua, LI Ya-Wei, MAO Lin-Chun. Effects of maize and soybean intercropping on soil physicochemical properties and microbial carbon metabolism in karst region [J]. Acta Agronomica Sinica, 2025, 51(1): 273-284.
[15]	DING Shu-Qi, CHENG Tong, WANG Bi-Kun, YU De-Bin, RAO De-Min, MENG Fan-Gang, ZHAO Yin-Kai, WANG Xiao-Hui, ZHANG Wei. Effects of planting density on photosynthetic production and yield formation of soybean varieties from different eras [J]. Acta Agronomica Sinica, 2025, 51(1): 161-173.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

QTL Analysis of Fatty acids Contents in Soybean

PDF

Cited