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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (12): 2073-2083.doi: 10.3724/SP.J.1006.2010.02073

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning of an ABC1-like Gene ZmABC1-10 and Its Responses to Cadmium and Other Abiotic Stresses in Maize (Zea mays L.)

GAO Qing-Song,YANG Ze-Feng,ZHOU Yong,ZHANG Dan,YAN Cheng-Hai,LIANG Guo-Hua,XU Chen-Wu*   

  1. Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology / Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China
  • Received:2010-05-18 Revised:2010-08-01 Online:2010-12-12 Published:2010-10-14
  • Contact: XU Chen-Wu, E-mail: qtls@yzu.edu.cn, Tel: +86-514-87979358; Fax: +86-514-87996817
  • Supported by:

    This study was supported by the National Basic Research Program of China (2006CB101700), the National Natural Science Foundation (30971846) and the Vital Project of Natural Science in Universities of Jiangsu Province, China (09KJA210002).

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Abstract: Cadmium is a non-essential heavy metal that is extremely toxic to plants and animals. Previous studies have shown that several proteins associated with the Activity of the bc1 complex (ABC1) protein family participate in plant responses to cadmium. Here we presented the cloning and characterization of an ABC1-like gene, ZmABC1-10, from maize (Zea mays L.). The full-length 2 519 bp cDNA of maize ABC1-10 gene contained an open reading frame (ORF) of 2 250 bp encoding a membrane-binding protein with a predicted localization in the chloroplast. A promoter scan detected numerous cis-elements implicated in abiotic stress, light, and phytohormone responses. Expression profile analysis indicated most expression of this gene occurred in green tissues. Cadmium treatment revealed that expression of this gene could be induced and was correlated with plant development. In addition to cadmium, ZmABC1-10 expression was also affected by a broad range of abiotic factors, such as ABA, H2O2, drought and darkness. A total of 19 members of maize ABC1 family were identified with the B73 maize genomic sequence. Phylogenetic analysis using 148 ABC1 proteins from 8 representative species of plant kingdom revealed that divergence occurred and species-specific expansion contributed to the evolution of this family in plants. Collectively, our data suggest that ZmAbc1-10 is a cadmium- esponsive factor and may play potential roles in the plant adaption to diverse abiotic stresses.

Key words: Maize, ABC1-like gene, Cloning, Cadmium response, Abiotic stress

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