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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (4): 636-644.doi: 10.3724/SP.J.1006.2010.00636

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Heat Shock Protein 70 May Improve the Ability of Antioxidant Defense Induced by the Combination of Drought and Heat in Maize Leaves

HU Xiu-Li1,LI Yan-Hui1,YANG Hai-Rong1,LIU Qun-Jun,LI Chao-Hai2,*   

  1. 1 College of Life Science, Henan Agricultural University; 2 College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2009-08-26 Revised:2009-12-22 Online:2010-04-12 Published:2010-02-05
  • Contact: LI Chao-Hai,E-mail: Chaohai@371.net

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Abstract:

In order to determine the mechanism of heat shock protein (HSP70) increasing crops endurance to the combination of drought and heat, we investigated the physiological characteristics of four maize varieties with different responses to drought and heat stresses. The results are as follows: (1) Under drought, heat, the combination of drought and heat, the increase of malondialdelehyde (MDA) content in leaf was the lowest in Zhuyu 309 without endurance to three stresses and the highest in Longyu 602 with endurance to three stresses; under drought, leaf MDA increase in Zhengdan958 with drought-endurance was less than that in Xundan 20 leaves with heat-endurance; under heat, leaf MDA increase was more in Zhengdan 958 than in Xundan20. (2) In Zhengdan958, Xundan20, Longyu602 leaves, the increase of ascorbate peroxidese (APX), glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT) activities was more than that in Zhuyu 309 leaves under drought, heat, the combination of drought and heat; under drought, APX, GR, SOD, CAT activities of Zhengdan 958 leaves increased significantly more than those of Xundan 20’, but those were contrary under heat; under the combination of drought and heat, APX, GR, SOD activities of Longyu 602 leaves increased significantly more than those of Zhengdan 958 and Xundan 20. (3) The pretreatment with HSP70 inhibitor quercetin (Q) significantly inhibited the increase of antioxidant enzymes activities in leaves of 4 maize varieties exposed to the three stresses. These results suggested that HSP70 involved in the increase of antioxidant enzyme activity in leaves of 4 maize varieties exposed to drought, heat and their combination, and APX, GR, SOD activities could be used as chemical and biological indicators to evaluate the crop endurance to drought, heat, and the combination of drought and heat stresses.

Key words: Maize(Zea mays L.), Drought, Heat, Combination stress, HSP70

[1]Thomson A M, Brown R A, Rosenberg N J, Izaurralde R C, Benson V. Climate change impacts for the conterminous USA: An integrated assessment. Part 3. Dryland production of grain and forage crops. Clim Change, 2005, 69: 43-65

[2]Bray E A, Bailey-Serres J, Weretilnyk E. Responses to abiotic stresses. In: Gruissem W, Buchannan B, Jones R, eds. Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists, Rockville, MD, 2000. pp 1158-249

[3]Pingali P L. CIMMYT 1999-2000 Facts and Trends. Meeting World Maize Needs: Technological Opportunities and Priorities for the Public Sector. Mexico: CIMMYT, 2001. pp 1-60

[4]Dat J F, Pellinen R, Beeckman T, Cotte B V D, Langebartels C, Kangasja J, Inze R, Breusegem F V. Changes in hydrogen peroxide homeostasis trigger an active cell death process in tobacco. Plant J, 2003,33: 621-632

[5]Neil S L, Desikan R, Hancock J T. Hydrogen peroxide signling. Curr Opin Plant Biol, 2002, 5: 388-395

[6]Volkov R A, Panchuk I I, Mullineaux P M, Schöffl F. Heat stress-induced H2O2 is required for effective expression of heat shock genes in Arabidopsis. Plant Mol Biol, 2006, 61: 733-46

[7]Hu X L, Jiang M Y, Zhang J H, Tan M P, Zhang A Y. Cross-talk between Ca2+/CaM and H2O2 in abscisic acid-induced antioxidant defense in leaves of maize plants exposed to water stress. Plant Grow Regul, 2008, 55: 183-198

[8]Apel K, Hirt H. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol, 2004, 55: 373-399

[9]Basha E, Lee G J, Demeler B, Vierling E. Chaperone activity of cytosolic small heat shock proteins. Eur J Biochem, 2004, 271: 1426-1436

[10]Oono Y, Seki M, Nanjo T, Narusaka M, Fujita M, Satoh R, Satou M, Sakurai T, Ishida J, Akiyama K, Iida K, Maruyama K, Satoh S, Yamaguchi-Shinozaki K, Shinozaki K. Monitoring expression profiles of Arabidopsisgene expression during rehydration process after dehydration using ca. 7000 full-length cDNA microarray. Plant J, 2003, 34: 868-887

[11] Rizhsky L, Hongjian L, Mittler R. The combined effect of drought stress and heat shock on gene expression in tobacco. Plant Physiol, 2002, 130: 1143-1151

[12] Rizhsky L, Liang H, Shuman J, Shulaev V, Davletova S. When defense pathways collide. The response of Arabidopsis to a combination of drought and heat stress. Plant Physiol, 2004, 134: 1683-1696

[13] Cho E K, Choi Y J. Anuclear-localized HSP70 confers thermoprotective activity and drought-stress tolerance on plants. Biotechnol Lett, 2009, 31: 597-606

[14] Dat J, Vandenabeele S, VranováE, Van Montagu M, InzéD, Van Breusegem F. Dual action of the active oxygen species during plant stress responses. CMLS Cell Mol Life Sci, 2000, 57: 779-795

[15] Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci, 2000, 7: 405-410

[16] Jiang M Y, Zhang J H. Water stress-induced abscisic acid accumulation triggers the increased generation of reactive and up-regulates the activies of antioxidant enzymes in maize leaves. J Exp Bot, 2002, 53: 2401-2410

[17] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 1976, 72: 248-254

[18] Heath R L, Parker L. Photoperoxidation in isolated chloroplasts kinetics and stoichiometry of fatty acid peroxidation. Arch Biophys, 1968, 25: 189-198

[19] Orozco-Cárdenas M L, Ryan C A. Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway.Proc Natl Acad Sci USA, 1999, 96-6557: 6553

[20] Liang Y(梁颖), Wang S-G(王三根). The protection function of Ca2+ on the membrane of rice seedlings under low temperature stress. Acta Agron Sin 作物学报), 2001, 27(1): 59-63 (in Chinese with English abstract) 

[21] Ma S-Y(马淑英), Zhao M(赵明). Regulations of calcium on the salt tolerance in Arabidopsis. Acta Agron Sin (作物学报), 2006, 32(11): 1706-1711 (in Chinese with English abstract)

[22] Rizhsky L, Hongjian L, Mittler R. The combined effect of drought stress and heat shock on gene expression in tobacco. Plant Physiol, 2002, 130: 1143-1151

[23] Dat J F, Foyer C H, Scott I M. Changes in salicylic acid and antioxidants during induction of thermotolerance in mustard seedlings. Plant Physiol, 1998, 118: 1455-1461

[24] Aebi H. Catalase in vitro. Methods Enzymol, 1984, 105: 121-125

[25] Gulli M, Rampino P, Lupotto E, Marmiroli N, Perrotta C. The effect of heat stress and cadmium ions on the expression of a small hsp gene in barley and maize. J Cereal Sci, 2005, 42: 25-31

[26] Guo S H, Wharton W, Moseley P, Shi H L. Heat shock protein 70 regulates cellular redox status by modulating glutathione-related enzyme activities. Cell Stress & Chaperones, 2007, 12: 245-254

[27] Bukau B, Weissman J, Horwich A. Molecular chaperones and protein quality control. Cell, 2006, 125: 443-451

[28] Voellmy R, Boellmann F. Chaperone regulation of the heat shock protein response. Adv Exp Med Biol, 2007, 594: 89-99

[29] Wang C R, Wang X R, Tian Y, Xue Y G, Xu X H, Sui Y X, Yu H X. Oxidative stress and potential biomarkers in tomato seedlings subjected to soil lead contamination. Ecotoxicol Environ Saf, 2008, 71: 685-691

[30] Wang C R, Wang X R, Tian Y, Yu H X, Gu X Y, Du W C, Zhou H. Oxidative stress, defense response, and early biomarkers for lead-contaminated soil in Vicia faba seedlings. Environ Toxicol Chem, 2008, 27: 970-977
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