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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (06): 975-981.doi: 10.3724/SP.J.1006.2011.00975

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Pyramiding Resistance Gene Pi-ta, Pi-b, and Stv-bi by Marker-assisted Selection in Rice (Oryza sativa L.)

WANG Jun,YANG Jie,CHEN Zhi-De,FAN Fang-Jun,ZHU Jin-Yan,YANG Jin-Huan,ZHONG Wei-Gong*   

  1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China
  • Received:2010-11-05 Revised:2011-03-28 Online:2011-06-12 Published:2011-04-12
  • Contact: 仲维功, E-mail: wgzhong0503@yahoo.com.cn

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Abstract: The blast and stripe disease are two major diseases of rice in Yangtze River japonica cultivating area. Breeding resistant cultivars is the most effective way to prevent these two major diseases. Wuyujing 8, containing both Pi-ta and Pi-b, was crossed with Zhendao 42 which carried rice stripe disease resistance gene Stv-bi. Two gene-markers, Pita and Pib, and one SCAR marker tightly linked with Stv-bi were used for marker-assisted selection in each segregating generation. After the multi-generation breeding and identification of resistance, three resistance genes were put together finally. One new rice stable line, 74121 was obtained which conferred good agronomic characteristics containing high-yield, good quality and multi-resistance. These results indicated that marker-assisted selection could not only be a simple and effective way but also provide genetic resources for breeding new rice multi-resistant varieties.

Key words: Rice, Blast, Stripe disease, Pi-ta, Pi-b, Stv-bi, Marker-assisted selection

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