Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (06): 975-981.doi: 10.3724/SP.J.1006.2011.00975

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Pyramiding Resistance Gene Pi-ta, Pi-b, and Stv-bi by Marker-assisted Selection in Rice (Oryza sativa L.)

WANG Jun,YANG Jie,CHEN Zhi-De,FAN Fang-Jun,ZHU Jin-Yan,YANG Jin-Huan,ZHONG Wei-Gong*   

  1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China
  • Received:2010-11-05 Revised:2011-03-28 Online:2011-06-12 Published:2011-04-12
  • Contact: 仲维功, E-mail: wgzhong0503@yahoo.com.cn

PDF

1858

Cited

7

Abstract: The blast and stripe disease are two major diseases of rice in Yangtze River japonica cultivating area. Breeding resistant cultivars is the most effective way to prevent these two major diseases. Wuyujing 8, containing both Pi-ta and Pi-b, was crossed with Zhendao 42 which carried rice stripe disease resistance gene Stv-bi. Two gene-markers, Pita and Pib, and one SCAR marker tightly linked with Stv-bi were used for marker-assisted selection in each segregating generation. After the multi-generation breeding and identification of resistance, three resistance genes were put together finally. One new rice stable line, 74121 was obtained which conferred good agronomic characteristics containing high-yield, good quality and multi-resistance. These results indicated that marker-assisted selection could not only be a simple and effective way but also provide genetic resources for breeding new rice multi-resistant varieties.

Key words: Rice, Blast, Stripe disease, Pi-ta, Pi-b, Stv-bi, Marker-assisted selection

[1]Wang C-L(王才林), Zhang Y-D(张亚东), Zhu Z(朱镇), Zhao L(赵凌), Chen T(陈涛). Rice breeding for resistance to stripe virus disease. Acta Agron Sin (作物学报), 2008, 34(3): 530–533 (in Chinese with English abstract)
[2]Ou S H. Rice Diseases, 2nd edn. UK: CAB International, 1985. pp 109–201
[3]Bryan G T, Wu K S, Farrall L, Jia Y L, Hershey H P, McAdams S A, Faulk K N, Donaldson G K, Tarchini R, Valent B. A single amino acid difference distinguishes resistant ansusceptible alleles of the rice blast resistance gene Pi-ta. Plant Cell, 2000, 12: 2033–2046
[4]Wang Z X, Yano M, Yamanouchi U, Iwamoto M, Monna1 L, Hayasaka H, Katayose Y, Sasaki T. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes. Plant J, 1999, 19: 55–64
[5]Yao S(姚姝), Chen T(陈涛), Zhang Y-D(张亚东), Zhu Z(朱镇), Zhao L(赵凌), Zhao Q-Y(赵庆勇), Zhou L-H(周丽慧), Wang C-L(王才林). Pyramiding of translucent endosperm mutant gene Wx-mq and rice stripe disease resistance gene, Stv-bi by marker-assisted selection in rice (Oryza sativa). Chin J Rice Sci (中国水稻科学), 2010, 24(4): 341–347 (in Chinese with English abstract)
[6]Hayano-Saito Y, Tsuji T, Fujii K, Saito K, Iwasaki M, Saito A. Localization of the rice stripe disease resistance gene, Stv-bi, by graphical genotyping and linkage analyses with molecular markers. Theor Appl Genet, 1998, 96: 1044–1049
[7]Hayano-Saito Y, Saito K, Nakamura S, Kawasaki S, Iwasaki M. Fine physical mapping of the rice stripe resistance gene locus, Stv-bi. Theor Appl Genet, 2000, 101: 59-63
[8]Hayano-Saito Y, Saito K, Fujii K, Touyama T, Tsuji T, Sugiura N, Izawa T, Iwasaki M. SCAR marker for selection of the rice stripe resistance gene Stv-bi. J Breed Res, 2000, 2: 67–72
[9]Luo C-P(罗楚平), Ni L(倪磊), Chen Z-Y(陈志谊), Liu Y-F(刘永锋), Liu Y-Z(刘邮洲), Nie Y-F(聂亚锋). Inoculation technology of rice blast and rice resistance to it in Jiangsu regional tests in 2009. Jiangsu Agric Sci (江苏农业科学), 2009, (6): 178–179 (in Chinese)
[10]Zhou T(周彤), Zhou Y-J(周益军), Cheng Z-B(程兆榜), Yang R-M(杨荣明), Ji J-A(吉健安). Identification of resistance to rice stripe virus in japonica rice varieties and analysis for its inhertance of Zhendao 88. Acta Phytophyl Sin (植物保护学报), 2007, 34(5): 475–479 (in Chinese with English abstract)
[11]Wang Z-H(王忠华), Jia Y-L(贾育林), Wu D-X(吴殿星), Xia Y-W(夏英武). Molecular markers-assisted selection of the rice blast resistance gene Pi-ta. Acta Agron Sin (作物学报), 2004, 30(12): 1259–1265 (in Chinese with English abstract)
[12]Fjellstrom R, Conaway-Bormans C A, McClung A M, Marchetti M A, Shank A R, Park W D. Development of DNA markers suitable for marker assisted selection of three Pi genes conferring resistance to multiple Pyricularia grisea pathotypes. Crop Sci, 2004, 44: 1790–1798
[13]Wang J(王军), Yang J(杨杰), Chen Z-D(陈志德), Zhong W-G(仲维功). Development and application of fragrance gene markers in rice. Mol Plant Breed (分子植物育种), 2008, 6(6): 1209–1212 (in Chinese with English abstract)
[14]Lei C-L(雷财林), Ling Z-Z(凌忠专), Wang J-L(王久林), Wan J-M(万建民). Research advanccs in rice breeding for disease resistance. Bull Biol (生物学通报), 2004, 39(11): 4–7 (in Chinese)
[15]Chen H-Q(陈红旗), Chen Z-Q(陈宗祥), Ni S(倪深), Zuo S-M(左示敏), Pan X-B(潘学彪), Zhu X-D(朱旭东). Pyramiding three genes with resistance to blast by marker-assisted selection to improve rice blast resistance of Jin23B. Chin J Rice Sci (中国水稻科学), 2008, 22(1): 23–27 (in Chinese with English abstract)
[16]Chen X-W(陈学伟), Li S-G(李仕贵), Ma Y-Q(马玉清), Li H-Y(黎汉云), Zhou K-D(周开达), Zhu L-H(朱立煌). Marker-assisted selection and pyramiding for three blast resistance genes, Pi-d(t)1, Pi-b, Pi-ta2, in rice. Chin J Biotech (生物工程学报), 2004, 20(5):708–713 (in Chinese with English abstract)
[17]Yoshimura S, Yoshimura A, Iwata N, McCouch S R. Tagging and combining bacterial blight resistance gene in rice using RAPD and RFLP markers. Mol Breed, 1995, 1: 375–387
[18]Huang N, Angeles E R, Domingo J, Magpantay G, Singh S, Zhang G, Kumaravadivel N, Bennett J, Khush G S. Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR. Theor Appl Genet, 1997, 95: 313–320
[19]Deng Q-M(邓其明), Zhou Y-J(周宇爝), Jiang Z-X(蒋昭雪), Wan Y-X(万映秀), Zhao B(赵斌), Yang L(杨丽), Li P(李平). Pyramiding bacterial blight (BB) resistance genes (Xa21, Xa4 and Xa23) into rice and its effect analysis. Acta Agron Sin (作物学报), 2005, 31(9): 1241−1246 (in Chinese with English abstract)
[20]Domingo J P, New T W, Kumaravaclivel N G, Magpantay G B, Hittalmani S, Parco A S, Huang N, Angeles E R, Khush G S. Rice resistant breeding to bacterial blight and blast by molecular marker-assisted selection. Philippine J Crop Sci, 1995, 20: 3
[21]Narayanar N N, Baisakh N, Vera Cruz C M, Gnanamanickam S S, Datta K, Datta S K. Improvement rice resistance to bacterial blight and blast through molecular breeding. Crop Sci, 2002, 42: 2072–2079
[22]Ni D-H(倪大虎), Yi C-X(易成新), Li L(李丽), Wang X-F(汪秀峰), Zhang Y(张毅), Zhao K-J(赵开军), Wang C-L(王春连), Zhang Q(章琦), Wang W-X(王文相), Yang J-B(杨剑波). Developing rice lines resistant to bacterial blight and blast with molecular marker-assisted selection. Acta Agron Sin (作物学报), 2008, 34(1): 100–105 (in Chinese with English abstract)
[23]Sugiura N, Tsuji T, Fujii K, Kato T, Saka N, Touyama T, Hayano-Saito Y, Izawa T. Molecular marker-assisted selection in a recurrent backcross breeding for the incorporation of resistance to rice stripe virus and panicle blast in rice (Oryza sativa L.). Breed Res, 2004, 6: 143–148 (in Japanese with English abstract)
[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[3] ZHENG Chong-Ke, ZHOU Guan-Hua, NIU Shu-Lin, HE Ya-Nan, SUN wei, XIE Xian-Zhi. Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1389-1400.
[4] ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[5] ZHENG Xiao-Long, ZHOU Jing-Qing, BAI Yang, SHAO Ya-Fang, ZHANG Lin-Ping, HU Pei-Song, WEI Xiang-Jin. Difference and molecular mechanism of soluble sugar metabolism and quality of different rice panicle in japonica rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1425-1436.
[6] YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[7] YANG Jian-Chang, LI Chao-Qing, JIANG Yi. Contents and compositions of amino acids in rice grains and their regulation: a review [J]. Acta Agronomica Sinica, 2022, 48(5): 1037-1050.
[8] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[9] YANG De-Wei, WANG Xun, ZHENG Xing-Xing, XIANG Xin-Quan, CUI Hai-Tao, LI Sheng-Ping, TANG Ding-Zhong. Functional studies of rice blast resistance related gene OsSAMS1 [J]. Acta Agronomica Sinica, 2022, 48(5): 1119-1128.
[10] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[11] WANG Xiao-Lei, LI Wei-Xing, OU-YANG Lin-Juan, XU Jie, CHEN Xiao-Rong, BIAN Jian-Min, HU Li-Fang, PENG Xiao-Song, HE Xiao-Peng, FU Jun-Ru, ZHOU Da-Hu, HE Hao-Hua, SUN Xiao-Tang, ZHU Chang-Lan. QTL mapping for plant architecture in rice based on chromosome segment substitution lines [J]. Acta Agronomica Sinica, 2022, 48(5): 1141-1151.
[12] WANG Ze, ZHOU Qin-Yang, LIU Cong, MU Yue, GUO Wei, DING Yan-Feng, NINOMIYA Seishi. Estimation and evaluation of paddy rice canopy characteristics based on images from UAV and ground camera [J]. Acta Agronomica Sinica, 2022, 48(5): 1248-1261.
[13] KE Jian, CHEN Ting-Ting, WU Zhou, ZHU Tie-Zhong, SUN Jie, HE Hai-Bing, YOU Cui-Cui, ZHU De-Quan, WU Li-Quan. Suitable varieties and high-yielding population characteristics of late season rice in the northern margin area of double-cropping rice along the Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(4): 1005-1016.
[14] CHEN Yue, SUN Ming-Zhe, JIA Bo-Wei, LENG Yue, SUN Xiao-Li. Research progress regarding the function and mechanism of rice AP2/ERF transcription factor in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 781-790.
[15] WANG Lyu, CUI Yue-Zhen, WU Yu-Hong, HAO Xing-Shun, ZHANG Chun-Hui, WANG Jun-Yi, LIU Yi-Xin, LI Xiao-Gang, QIN Yu-Hang. Effects of rice stalks mulching combined with green manure (Astragalus smicus L.) incorporated into soil and reducing nitrogen fertilizer rate on rice yield and soil fertility [J]. Acta Agronomica Sinica, 2022, 48(4): 952-961.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd