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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (06): 1054-1059.doi: 10.3724/SP.J.1006.2013.01054

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Excavation and Characterization of Novel Rice 57H Mutants

CHE Qian-Qian1,GUO Yan-Ping1,LI Qiang1,WANG Yi-Hua2,WANG Guang-Yuan3,TIAN Huai-Dong1,*   

  1. 1 College of Life Science, Shanxi University, Taiyuan 030006, China; 2 State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China; 3 Shanxi Institute of Crop Science, Taiyuan 030031, China
  • Received:2012-12-04 Revised:2013-01-15 Online:2013-06-12 Published:2013-03-22

Abstract:

Glutelins and prolamins, the main rice storage proteins, are synthesized in the ER and accumulated into the vacuolar protein body and the ER-derived protein body in endosperm cell of the developing seed of the wild type rice, respectively. Several 57H mutations have been found to cause the high increase of 57 kD glutelin precursor and the remarkable decrease of the mature glutelins, and involved in the accumulation of storage proteins in rice. In this study, two 57H mutants were newly found and obtained from rice germplasms using SDS-PAGE technique. SDS-PAGE analysis of the total seed proteins showed that the two mutants had the characters namely the great increase of 57 kD polypeptide and the lack of 13 kD polypeptide, but not alteration of characters for the mature glutelins and other storage proteins; Electrophoretic and immuno-blotting analyses of glutelin showed that the increased polypeptides was 57 kD glutelin precursor in the mutants; Electrophoretic analysis of prolamins showed that the lacked polypeptide was 13kD prolamin in them. It is indicate that the two mutants have novel characters distinct from the reported 57H mutants. They probably contain unknown genetic information for synthesis and expression of storage proteins, and are considered to be useful materials for the overall understanding of genetic mechanism regulating synthesis and accumulation of the storage proteins in rice.

Key words: Rice, Endosperm, Storage proteins, Novel 57H mutants

 


 


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