Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (10): 1835-1842.doi: 10.3724/SP.J.1006.2013.01835

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Mechanisms of Tiller Occurrence Affected by Exogenous IAA, GA3, and ABA in Wheat with Different Spike-types

CAI Tie,XU Hai-Cheng,YIN Yan-Ping,YANG Wei-Bing,PENG Dian-Liang,NI Ying-Li,XU Cai-Long,YANG Dong-Qing,WANG Zhen-Lin*   

  1. State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China.
  • Received:2013-01-21 Revised:2013-06-01 Online:2013-10-12 Published:2013-07-31
  • Contact: 王振林, E-mail: zlwang@sdau.edu.cn, zlwangsd@sina.com, Tel: 0538-8241359

Abstract:

Two winter wheat cultivars, Shannong 15 and Shannong 8355, were used to study the effects of exogenous indole-3-acetic acid (IAA), gibberellic acid 3 (GA3), and abscisic acid (ABA) on tiller occurrence in wheat and the underlying mechanism. Exogenous IAA and GA3 inhibited occurrence of wheat tiller, while exogenous ABA reduced the rate of tiller occurrence. Furthermore, exogenous IAA and GA3 completely inhibited growth of tiller bud, while exogenous ABA significantly slowed the growth rate of tiller bud. The growth of tiller bud was positively correlated with content ofzeatin (ZT), but negatively correlated with IAA content and ratios of IAA/ZT and ABA/ZT. In contrast, the growth of tiller bud had slightly negative correlations with GA3 and ABA contents. These results suggest that IAA and ZT play a key role in regulating tiller occurrence in wheat, and exogenous hormones regulate the growth of tiller bud through changing contents of IAA and ZT and the ratios of IAA/ZT and ABA/ZT in tiller node.

Key words: Wheat, Tiller bud, Plant hormones, Regulation

[1]Li X Y, Qian Q, Fu Z M, Wang Y H, Xiong G S, Zeng D L, Wang X Q, Liu X F, Teng S, Hiroshi F, Yuan M, Luo D, Han B, Li J Y. Control of tillering in rice. Nature, 2003, 422: 618−621



[2]Liu Y, Gu D D, Ding Y F, Wang Q S, Li G H, Wang S H. The relationship between nitrogen, auxin and cytokinin in the growth regulation of rice (Oryza sativa L.) tiller buds. Aust J Crop Sci, 2011, 5: 1019–1026



[3]Chatfield S P, Stirnberg P, Forde B G, Leyser O. The hormonal regulation of axillary bud growth in Arabidopsis. Plant J, 2000, 24: 159−169



[4]Shimizu S S, Mori H. Control of outgrowth and dormancy in axillary buds. Plant Physiol, 2001, 127: 1405−1413



[5]Wang G, Romheld V, Li C, Bangerth F. Involvement of auxin and CKs in boron deficiency induced changes in apical dominance of pea plants. J Plant Physiol, 2006, 163: 591−600



[6]Leopold A. The control of tillering in grasses by auxin. Am J Bot, 1949, 36: 437−440



[7]Langer R, Prasad P, Laude H. Effects of kinetin on tiller bud elongation in wheat (Triticum aestivum L.). Ann Bot, 1973, 37: 565−571 



[8]Rood, S. B. Application of gibberellic acid to control tillering in early-maturing maize. Can J Plant Sci, 1985, 65: 901−911



[9]Hong X-F(洪晓富), Jiang P-Y(蒋彭炎), Zheng Z-S(郑寨生), Lu C-Y(卢昌银), Wang C-M(王撮明). Relationships between employ GA3 during tillering stage and promote the panicle bearing tiller rate. J Zhejiang Agric Sci (浙江农业科学), 1998, (1): 3−5 (in Chinese with English abstract)



[10]Zhang G P. Gibberellic acid 3 modifies some growth and physiologic effects of paclobutrazol (PP333) on wheat. J Plant Growth Regul, 1997, 16: 21−25



[11]Phillips I D J. Apocaldominance. Annu Rev J Plant Physiol, 1975, 26: 341−347



[12]Emery R J N, Longnecler N E, Atkins C A. Branch development in Lupinus angustifolius L.: II. Relationship with endogenous ABA, IAA and CKs in axillary and main stem buds. J Exp Bot, 1998, 49: 555−562



[13]Wang R-F(王如芳), Zhang J-W(张吉旺), Lü P(吕鹏), Dong S-T(董树亭), Liu P(刘鹏), Zhao B(赵斌). Effects of endogenous hormones on tiller development process of different maize varieties. Sci Agric Sin (中国农业科学), 2012, 45(5): 840−847 (in Chinese with English abstract)



[14]Wang R-F(王如芳), Zhang J-W(张吉旺), Lü P(吕鹏), Dong S-T(董树亭), Liu P(刘鹏), Zhao B(赵斌). Tillering characteristics of multi-tiller maize and influence of plant density and sowing date. Acta Agron Sin (作物学报), 2012, 38(2): 322−332 (in Chinese with English abstract)



[15]Liu Y(刘杨), Ding Y-F(丁艳锋), Wang Q-S(王强盛), Li G-H(李刚华), Xu J-X(许俊旭), Liu Z-H(刘正辉), Wang S-H(王绍华). Effect of plant growth regulators on the growth of rice tiller bud and the changes of endogenous hormones. Acta Agron Sin (作物学报), 2011, 37(4): 670−676 (in Chinese with English abstract)



[16]Liu Y(刘杨), Wang Q-S(王强盛), Ding Y-F(丁艳锋), Liu Z-H(刘正辉), Li G-H(李刚华), Wang S-H(王绍华). Effect of nitrogen and 6-BA on development of tillering bud and its physiological mechanism. Acta Agron Sin (作物学报), 2009, 35(10): 1893−1899 (in Chinese with English abstract)



[17]Ma X-L(马兴林), Liang Z-X(梁振兴). Studies on the effects of endogenous hormones in winter wheat tillers during the course of senescence. Acta Agron Sin (作物学报), 1997, 23(2): 200−207 (in Chinese with English abstract)



[18]Li C-X(李春喜), Zhao G-C(赵广才), Dai X-M(代西梅), Jiang L-N(姜丽娜), Shang Y-L(尚玉磊). Research on the relationship between wheat tillering dynamics and endogenous hormone. Acta Agron Sin (作物学报), 2000, 26(6): 963−968 (in Chinese with English abstract)



[19]Yang T-X(杨途熙), Wei A-Z(魏安智), Zheng Y(郑元), Yang H(杨恒), Yang X-N(杨向娜), Zhang R(张睿). Simultaneous determination of endogenous hormone sin apricot floral bud by high performance liquid chromatography. Chin J Anal Chem (分析化学), 2007, 35(9): 1359−1361 (in Chinese with English abstract)



[20]Yang W-B(杨卫兵), Wang Z-L(王振林), Yin Y-P(尹燕枰), Li W-Y(李文阳), Li Y(李勇), Chen X-G(陈晓光), Wang P(王平), Chen E-Y(陈二影), Guo J-X(郭俊祥), Cai T(蔡铁), Ni Y-L(倪英丽). Effects of spraying exogenous ABA or GA on the endogenous hormones concentration and filling of wheat grains. Sci Agric Sin (中国农业科学), 2011, 44(13): 2673−2682 (in Chinese with English abstract)



[21]Liu Y, Ding Y F, Wang Q S, Meng D X, Wang S H. Effects of nitrogen and 6-Benzylaminopurine on rice tiller bud growth and changes in endogenous hormones and nitrogen. Crop Sci, 2011, 51: 786–792



[22]Wang Y-S(王永胜), Wang J(王景), Duan J-Y(段静雅), Wang J-F(王金发), Liu L-S(刘良式). Isolation and genetic research of a dwarf tillering mutant rice. Acta Agron Sin (作物学报), 2002, 28(2): 235−239 (in Chinese with English abstract)



[23]Tang J-B(唐家斌), Zeng W-Y(曾万勇), Wang W-M(王文明), Ma B-Q(马炳田), Liu Y(刘勇), Li H-J(李浩杰), Xia H-A(夏红爱), Li P(李平), Zhu L-H(朱立煌). Genetic research and genetic supports of a few tiller mutant rice. Sci China (Ser C: Life Sci)(中国科学•C辑), 2001, 31(3): 208−212 (in Chinese)



[24]Yang W-Y(杨文钰), Han H-F(韩惠芳), Ren W-J(任万君), Zhao L(赵莉), Fan G-Q(樊高琼). Effects of uniconazole waterless-dressing seed on endogenous hormones and C/N ratio at tillering stage of wheat. Acta Agron Sin (作物学报), 2005, 31(6): 760−765 (in Chinese with English abstract)



[25]Wang S-H(王绍华), Jie S-T(揭水通), Ding Y-F(丁艳锋), Wang Q-S(王强盛). Effects of tiller inhibitors on regulation of rice tillering. Jiangsu J Agric Sci (江苏农业学报), 2002, 18(1): 29−32 (in Chinese with English abstract)



[26]Zhou C-F(周传凤), Li Y-R(李杨瑞), Yang L-T(杨丽涛). Effect of ethephon sprayed at early tillering stage on the activities of peroxidase, IAA oxidase and acid invertase in sugarcane in correlation to tillering. Guihaia (广西植物), 2007, 27(4): 649−652 (in Chinese with English abstract)



[27]Woodward E, Marshall C. Effects of plant growth regulators and nutrient supply on tiller bud outgrowth in barley (Hordeum distichum L.). Ann Bot, 1998, 61: 347−354



[28]Morris G C, Choonseok O H. A reappraisal of the role of abscisic acid and its interaction with auxin in apical dominance. Ann Bot, 2006, 98: 891−897



[29]Michael G, Beringer H. The role of hormones in yield formation, physiological aspects of group productivity. In: Proceedings of the Colloquium of the International Potash Institute, Wageninien, The Netherlands, 1980. pp 252−279



[30]Tanaka M, Takei K, Kojima M, Sakakibara H, Mori H. Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance. Plant J, 2006, 45: 1028−1036 



[31]Bangerth F. Response of CK concentration in the xylem exudates of bean (Phaseolus vulgaris L.) plants to decapitation and auxin treatment, and relationship to apical dominance. Planta, 1994, 194: 439−442



[32]Liang Z-X(梁振兴), Ma X-L(马兴林). Studies on the effects of endogenous hormones on tiller development process of winter wheat. Acta Agron Sin (作物学报), 1998, 24(6): 788−792 (in Chinese with English abstract)



[33]Cline M G. Exogenous auxin effects on lateral bud outgrowth in decapitated shoots. Ann Bot, 1996, 78: 256−266



[34]Cline M G, Chatfield S P, Leyser O. NAA restores apical dominance in the axr3-1 mutant of Arabidopsis thaliana. Ann Bot, 2001, 87: 61−65



[35]Shang Y-L(尚玉磊), Li C-X(李春喜); Shao Y(邵云), Jiang L-N(姜丽娜). Comparison of dynamics and functions of endogenous IAA, CTK content among main crops of gramineae at early growing stage. Acta Agricul Boreali-Sin (华北农学报), 2004, 19(4): 47−50 (in Chinese with English abstract)



[36]Zhang Z-D(张祖德). Research of chemical regulation on promote the panicle bearing tiller rate of rice. Fujian Sci Tech Rice Wheat (福建稻麦科技), 2006, (6): 10−13 (in Chinese)



[37]Chrispeels M, Varner J. Inhibition of gibberellic acid induced formation of α-amylase by abscisin II. Nature, 1966, 212: 1066−1067



[38]Pei Z M, Ghassemian M, Kwak C M. Role of farnesyltransferase in ABA regulation of guard cell anion channels and plant water loss. Science, 1998, 282: 287−290



[39]Wang G-M(王光明), Liu B-G(刘保国), Chen J(陈静), Ren C-F(任昌福). Effects of endogenous ABA on the germination of ratooning buds in paddy rice. J Southwest Agric Univ (西南农业大学学报), 1997, 19(4): 338−342 (in Chinese with English abstract)



[40]Xu L-N(徐丽娜), Feng W(冯伟), Sheng K(盛坤), Zhu Y-J(朱云集), Ma D-Y(马冬云), Guo T-C(郭天财) Difference of endogenous hormones in Leaves between main stem and tillers and its effect on spike formation in large spike winter wheat cultivar Lankao Aizao 8 under different plant densities. Acta Agron Sin (作物学报), 2010, 36(9): 1596−1604 (in Chinese with English abstract)

[1] Zhai Sheng-Nan, Cao Xin-You, Li Hao-Sheng, Li Ji-Hu, Li Fa-Ji, Liu Jin-Dong, Xia Xian-Chun, Lyu Ying-Ying, Ma Rui-Feng, Wang Ying, Geng Hong-Wei, Liu Jian-Jun. Analysis of the genetic effects of allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci on peroxidase activity in wheat grains [J]. Acta Agronomica Sinica, 2026, 52(6): 1593-1603.
[2] Xi Qian-Hui, Xu Zi-Yuan, Liu Meng-Meng, Wang Hong-Yi, Lang Kai-Lin, Jing Zhen-Hai, Chen Feng, Zhao Lei. Genome-wide association study and candidate gene prediction of grain copper content in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1604-1617.
[3] Mao Jia-Qi, Huang Peng-Yu, Zhao Jia-Jia, Zheng Xing-Wei, Wu Bang-Bang, Hao Yu-Qiong, Qu Fei, Liu Cheng, Ma Peng-Tao, Zheng Jun. Evaluation of powdery mildew resistance in wheat cultivars and molecular detection of resistance genes in Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(6): 1669-1681.
[4] Hu Chuan, Zhao Kai-Nan, Huang Xiu-Li, Wu Jin-Zhi, Ren Kai-Ming, Wang He-Zheng, Fu Guo-Zhan, Huang Ming, Li You-Jun. Effects of tillage methods and nitrogen rates on yield and quality of dryland wheat under one-off irrigation [J]. Acta Agronomica Sinica, 2026, 52(6): 1830-1846.
[5] Chen Xue-Yan, He Hua-Chuan, Li Zheng-Jia, Dong Xin-Pan, Li Ou-Qi, Liu Xiao-Yun, Li Dan-Ping, Chen Zhi-Wei, Liu Guo-Xia, Lyu Sheng-Yuan, Wu Yin-Ying, Zhao Zhen-Dong, Cao Xin-You, Wan He-Ping. Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in ‘Jimai 60’ seedlings under combined salinity-alkalinity stress in hydroponics [J]. Acta Agronomica Sinica, 2026, 52(6): 1859-1875.
[6] Gao Pei-Yang, Li Jin-Xuan, Dong Yu-Kui, Shi Yu, Zhang Zhen, Zhang Yong-Li. Response of wheat tillering and spike formation to nitrogen rate under supplementary irrigation based on soil moisture content [J]. Acta Agronomica Sinica, 2026, 52(6): 1847-1858.
[7] Zhang Xian-Feng, Guo Li-Jian, Li Kang-Chun, Kong Bin-Xue, Liu Yu-Fang, Che Zhuo, Yang De-Long. Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1711-1727.
[8] Wang Zhuang-Zhuang, Wu Zi-Jun, Zhang Yong-Xin, Zhang Xin-Yuan, Yuan Li-Xue, Chen Ru-Xue, Liu Shi-Ju, Duan Jian-Zhao, Feng Wei, Wang Tong-Chao, Wang Yong-Hua. Optimized water-nitrogen synergy enhances winter wheat yield and nitrogen use efficiency in clay-loam fluvo-aquic soils of southeastern Henan, China [J]. Acta Agronomica Sinica, 2026, 52(5): 1501-1521.
[9] Zhang Zhen, Feng Lian-Jie, Shi Yu, Yu Zhen-Wen, Zhang Yong-Li. Yield formation of wheat with different ear types under water-saving supplementary irrigation conditions [J]. Acta Agronomica Sinica, 2026, 52(5): 1522-1535.
[10] He Wan-Long, Geng Hong-Wei, Zhang Fei-Fei, Mikereayi·Ababaikere , Luo Zi-Yang, Li Peng-Cheng, Zhou Zhao-Yu, Cheng Yu-Kun. Development of a deep learning-based image recognition system for major wheat diseases [J]. Acta Agronomica Sinica, 2026, 52(5): 1401-1417.
[11] Ma Hai-Hui, Zhang Guo-Ping, Yang Si-Cun, Wang Hong-Li. Effects of nitrogen application at different densities on carbon and nitrogen accumulation and translocation characteristics in forage maize in semi-arid regions [J]. Acta Agronomica Sinica, 2026, 52(4): 1193-1207.
[12] Hou Si-Yu, Wang Guo-Cui, Wei Jin-Gui, Xie Wei-Xin, Yin Wen, Fan Zhi-Long, Chai Qiang, Hu Fa-Long. Effects of green manure combined with chemical nitrogen fertilizer on dry matter accumulation and yield formation of wheat in arid irrigation areas of northwestern China [J]. Acta Agronomica Sinica, 2026, 52(4): 1208-1219.
[13] Shang Yun-Qiu, Zhao Zhu, Chen Huan, Ding Yong-Gang, Qiao Yu-Qiang, Li Wei, Zhang Xiang-Qian, Cao Cheng-Fu, Du Shi-Zhou. Effects of long-term tillage practices on grain-filling and yield formation in rain-fed wheat [J]. Acta Agronomica Sinica, 2026, 52(4): 1236-1250.
[14] Song Song-Quan, Tang Cui-Fang, Liang Yu-Rong, Cheng Hong-Yan, Wang Wei-Qing. Research progress in biosynthesis and action mechanism of sRNAs and their regulation on seed development, dormancy and germination [J]. Acta Agronomica Sinica, 2026, 52(4): 959-981.
[15] Qiao Yu-Xin, Li Cheng-Yue, Kang Xiao-Yu, Zhang Xin-Qi, Jia Shao-Hui, Liu Qian, Cao Ya-Li, Shi Xin-Rui, Hao Xing-Yu, Li Ping. Study on the effects of long-term no-tillage straw mulching on wheat yield improvement in dryland areas based on the APSIM model [J]. Acta Agronomica Sinica, 2026, 52(4): 1181-1192.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!