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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (11): 1944-1951.doi: 10.3724/SP.J.1006.2013.01944

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning, Characterization, and Functional Analysis of TaWRKY44 Gene from Wheat

WANG Rui1,2,**,WU Hua-Ling3,**,WANG Hui-Fang1,2,HUANG Ke1,2,HUO Chun-Yan1,2,NI Zhong-Fu1,2,SUN Qi-Xin1,2,*   

  1. 1 State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / China Agricultural University, Beijing 100193, China; 2 National Plant Gene Research Centre (Beijing), Beijing 100193, China; 3 Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
  • Received:2013-03-18 Revised:2013-06-25 Online:2013-11-12 Published:2013-08-12
  • Contact: 孙其信, E-mail: qxsun@cau.edu.cn

Abstract:

WRKY transcription factors are specific in plants and related in response to stress as well as plant development. In this study, we cloned the full-length cDNA of a new WRKY transcription factor gene, TaWRKY44, from wheat (Triticum aestivum L.). The open reading frame of TaWRKY44 is 897 bp in length, which encodes 298 animo acid residues. The semi-quantitative RT-PCR result indicated that TaWRKY44 was highly expressed in leaf, and responsive to drought and clod stresses. TaWRKY44 over-expressed lines of Arabidopsis exhibited smaller leaves, shorter leaf petioles, and smaller leaf epidermis cell than the wild type. In addition, the transgenic lines were more sensitive to abscisic acid, drought, and salt stresses. These results indicated TaWRKY44 could be a negative regulator in stress signal transduction pathway.

Key words: Wheat, WRKY, Gene expression, Leaf, Abiotic stress, Functional analysis

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