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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (04): 644-649.doi: 10.3724/SP.J.1006.2014.00644

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis and Gene Fine Mapping of Yellow-Green Leaf Mutant ygl80 in Rice

LI Yan-Qun1,**,GAO Jia-Xu1,**,XIAO Yun-Hua1,LI Xiu-Lan2,PU Xiang1,SUN Chang-Hui1,WANG Ping-Rong1,DENG Xiao-Jian1,*   

  1. 1 Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China; 2 College of Life Science, Qufu Normal University, Qufu 273165, China
  • Received:2013-08-02 Revised:2013-12-12 Online:2014-04-12 Published:2014-01-16
  • Contact: 邓晓建, E-mail: xjdeng2006@aliyun.com E-mail:Liyanqun63@163.com

Abstract:

A yellow-green leaf mutant ygl80 was isolated by chemical mutagenesis. Compared with the wild-type parent 10079, chlorophyll content of the ygl80 mutantdecreased by 76.64% and 54.59%, and the carotenoid content decreased by 53.85% and 41.18% at the seedling and booting stages, respectively. In addition, plant height, number of productive panicles per plant, number of spikelets per panicle, panicle length and 1000-grain weight reduced by 14.8%, 16.5%, 21.3%, 9.1%, and 7.4%, respectively, at the maturity. Genetic analysis showed that the yellow-green leaf trait of the ygl80 mutant was controlled by one pair of recessive nuclear genes. Genetic mapping of the mutant gene was conducted by using 627 yellow-green leaf individuals from the F2 mapping population of ygl80/Zhefu802. Finally, the mutant gene was mapped between InDel markers C2 and C3 on the long arm of chromosome 5, with genetic distances of 0.24 cM and 0.39 cM, respectively, and with physical distance of 90 kb, in this region eleven predicted genes had been annotated. Sequencing analysis of these candidate genes between the mutant and its wild-type parent revealed a single base change (C5027T) of YGL1 (LOC_Os05g28200) gene for chlorophyll synthase resulted in a missense mutation (P348L) in the encoded product, suggesting that the ygl80 mutant gene is allelic to the ygl1 gene. The ygl80 mutant exhibited yellow-green trait throughout the growing period. But the ygl1 mutant showed yellow-green trait at seedling stage, then turned into green slowly, and its leaf color and chlorophyll and carotenoid contents almost closed to those of the wild-type parent during the later stage of growth. Different phenotypes of the two mutants may be caused by different mutational sites of genomic sequenceof YGL1 gene encoding chlorophyll synthase.

Key words: Oryza sativa L., Yellow-green leaf mutant, YGL1, Genetic analysis, Fine mapping

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