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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (01): 51-62.doi: 10.3724/SP.J.1006.2017.00051

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Gene Mapping and Cloning of a Premature Leaf Senescence Mutant psls1 in Rice

HUANG Ya-Min1,**,ZHU Shan-Shan1,**,ZHAO Zhi-Chao1,**,PU Zhi-Gang2,LIU Tian-Zhen1, LUO-Sheng1,ZHANGXin1,*   

  1. 1National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2Biotechnology Institute of Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066?, China
  • Received:2016-03-18 Revised:2016-06-20 Online:2017-01-12 Published:2016-07-04
  • Contact: 张欣,E-mail: zhangxin02@caas.cn ** 同等贡献(Contributed equally to this work) E-mail:zhangxin02@caas.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (91535302) and the Major Project of China on New Varieties of GMO Cultivation (2015ZX08010-004).

Abstract:

Leaf early senescence directly reduces its photosynthetic capacity, decreasing crop yield and grain quality. It is of great importance to identify novel mutants and characterize their molecular and physiological mechanisms. In this paper, we reported gene mapping and cloning of a psls1 mutant (premature senescence leaf with spots) in rice. The mutant showed early senescence symptoms, including decreased chlorophyll content, over-accumulated H2O2, yellow-withered leaf and increased dead cell from bottom to top of plant after the 7-leaf stage. Moreover, the plant height, tiller number, panicle length, and the number of grains per panicle were significantly lower in psls1. We observedthe degradation of chloroplast, unclean thylakoid and numerous osmiophilic granules in psls1 leavesby transmission electron microscope (TEM). Genetic analysis demonstrated that the phenotype of psls1 was determined by a single recessive gene. Using genetic population derived from psls1 × IRAT129, the psls1 locus was mapped in a 89 kb region flanked by markers zs-3 and zs-8 on chromosome 7, containing 12 putative open reading frames (ORFs). Sequence analysis revealed a single-base substitution occurred in the genomic sequence of LOC_Os07g46460, which led to a 59 bp deletion in its cDNA, and therefore, predicted LOC_Os07g46460 (PSLS1), encoding a ferredoxin-dependent glutamate synthase as the candidate gene. The mRNA expression level of mutated PSLS1 decreased sharply, resulting in reduction of glutamate synthase activity and abnormal amino acid metabolism in psls1. Under low nitrogen treatment, the senescence phenotype of psls1 could occur as early as at the 3-leaf stage. These results indicated that psls1 senescence phenotype might be associated with the loss of glutamate synthase activity and the abnormal amino acid metabolism.

Key words: Rice, Leaf senescence, Gene-mapping, PSLS1 gene, Glutamate synthase (Fd-GOGAT)

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