甘蓝型油菜茎高QTL定位及株高相关位点整合

doi:10.3724/SP.J.1006.2019.84133

Abstract

Abstract:

Stem height (SH) is important for the plant architecture and yield in Brassica napus. To date, the research about stem height in B. napus is very less. In the study, QTL mapping of stem height in B. napus was performed using the high density SNP genetic map constructed from the recombinant inbred lines (RIL) population with Illumina 60K SNP array. Totally 11 QTLs were detected in two years on A04, A06, C04, A08, and C01, which explained 7.25%-19.61% of the phenotypic variation. In addition, genome-wide association analysis (GWAS) of stem height in the 455 B. napus genotyped by resequencing was performed, and five significantly associated SNP loci on A08, A10, C02, and C06 were identified. And the candidate genes involved in photomorphogenesis, plant growth and development, and hormone pathway such as auxin, gibberellin and brassinosteroid were identified. We also integrated the loci for plant height-related traits including plant height (PH), main inflorescence length (MIL), branch segment (BS) and branch initiation height (BIH) in B. napus, in which four loci for PH on A01, A03, A07, C03, and C06 were identified in at least four populations, one locus for MIL on A10 and two loci for BIH on A02 and C03 were identified in two populations. The loci for stem height in our study were consistent with pervious QTL mapping results for plant height, branch initiation height and main inflorescence length. This study lays a foundation for the breeding of plant architecture in B. napus.

Key words: Brassica napus, stem height, QTL mapping, GWAS, loci integration

Li-Juan WEI,Rui-Ying LIU,Li ZHANG,Zhi-You CHEN,Hong YANG,Qiang HUO,Jia-Na LI. Detection of stem height QTL and integration of the loci for plant height- related traits in B. napus[J].Acta Agronomica Sinica, 2019, 45(6): 818-828.

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Table 5

Identification of candidate genes for stem height"

位点名称 Name	基因 Genes	物理位置 Physical position	拟南芥同源基因 Homologs in A. thaliana	功能注释 Functional annotation
q2016SH-A04-2	BnaA04g25550D	A04: 18331184-18331589	AT2G44080/ARL	调控细胞大小 Regulated cell expansion
q2016SH-A04-3	BnaA04g24760D	A04: 18025141-18026983	AT2G43010/PIF4	编码转录因子bHLH, 参与生长素生物合成, 调控下胚轴伸长 Encoded the bHLH transcription factor, involving in the auxin biosynthesis and regulating hypocotyl elongation
	BnaA04g24770D	A04: 18030030-18033008	AT2G43020/PAO2	参与ABA介导的植物生长 Involved in plant development mediated by ABA
	BnaA04g24970D	A04: 18140559-18140805	AT2G43280/FAR1	调控光敏色素信号传导 Involved in phytochrome A signaling
	BnaA04g24980D	A04: 18141000-18144940	AT2G43280/FAR1	调控光敏色素信号传导 Involved in phytochrome A signaling
q2016SH-A06-2	BnaA06g03090D	A06: 1869158-1871192	AT1G49770/RGE1	编码转录因子bHLH,影响植物生长发育 Encoded the bHLH transcription factor, regulating plant growth and development
q2016SH-A06-4	BnaA06g13320D	A06: 6972730-6976892	AT1G19220/ARF19	参与生长素和乙烯信号传导过程 Involved in auxin and ethylene signaling
q2016SH-C04	BnaC04g10190D	C04: 7847368-7848334	AT2G35150/EXL7	调控植物生长发育 Regulated plant growth and development
q2017SH-A08	BnaA08g28200D	A08: 18580209-18583117	AT1G05180/AXR1	参与生长素抵抗反应 Involved in auxin resistance
q2017SH-C01-2	BnaC01g17440D	C01: 12009590-12010572	AT4G25560/MYB18	调控光形态建成 Regulated photomorphogenesis in the light
2016SH-1	BnaA08g15400D	A08: 12809202-12810946	AT4G36730/GBF1	调控光形态建成 Regulated photomorphogenesis in the light
2016SH-2	BnaA10g24860D	A10: 16153991-16157850	AT5G05690/CPD	编码CP90A蛋白, 参与油菜素内酯生物合成 Encoded a member of the CP90A family, and involved in the brassinosteroid biosynthesis
2017SH-1	BnaC02g17570D	C02: 13199941-13200513	AT5G13790/AGL15	MADS家族成员, 参与赤霉素代谢 Encoded a member of the MADS family, and involved in the gibberellin metabolic process
2017SH-2	BnaC06g27170D	C06: 28615555-28619464	AT5G37020/ARF8	参与生长素稳态 Involved in auxin homeostasis

Table 5

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	年份 Year	平均值±标准偏差 Mean±SD (cm)	范围 Range (cm)	变异系数 CV (%)
父本ZY821 Male parent ZY821	2016	138.00±5.51
	2017	131.90±12.30
母本GH06 Female parent GH06	2016	159.60±6.46
	2017	145.60±8.77
重组自交系RIL	2016	150.64±11.49	119.00-174.25	7.63
	2017	148.53±10.91	114.40-175.00	7.34
自然群体 Natural population	2016	156.58±17.11	93.86-200.10	10.93
	2017	143.01±21.20	62.00-202.60	14.82

性状 Trait	重组自交系 RIL		自然群体 Natural population
性状 Trait	2016SH	2017SH	2016SH	2017SH
主花序长度 Main infloresences length (cm)	-0.403^**	-0.355^**	-0.089	-0.306^**
株高 Plant height (cm)	0.738^**	0.617^**	0.865^**	0.865^**
结荚高度 Height of podding (cm)	0.638^**	0.581^**	0.745^**	0.850^**
结荚厚度 Thickness of pod canopy (cm)	0.089	0.024	0.185	0.101
一次有效分枝起点 Branch initiation height (cm)	0.610^**	0.589^**	0.621^**	0.772^**
经济产量 Yield (g)	0.341^**	0.037	0.255^**	0.286^**

位点 QTL	染色体 Chr.	LOD阈值 LOD score	标记区间 SNP interval	加性效应^a Additive effect	贡献率 R²(%)	物理区间 Physical position (bp)
q2016SH-A04-1	A04	3.72	SNP42542-SNP9367	6.21	13.70
q2016SH-A04-2		5.61	SNP9354-SNP9343	7.17	19.52	18264803-18455956
q2016SH-A04-3		2.90	SNP42544-SNP9263	5.61	10.78	17846583-18115323
q2016SH-A06-1	A06	4.33	SNP13035-SNP13048	-4.72	14.46	1469838-1480581
q2016SH-A06-2		5.50	SNP13263-SNP34194	-5.45	19.61	1860393-2106757
q2016SH-A06-3		4.56	SNP12979-SNP13009	-4.88	15.07	1360428-1442742
q2016SH-A06-4		2.83	SNP14849-SNP14835	3.65	8.88	6967630-7274382
q2016SH-C04	C04	3.29	SNP49157-SNP49146	3.85	9.97	7352388-7867004
q2017SH-A08	A08	3.94	SNP18566-SNP18577	2.95	7.25	18081289-18861275
q2017SH-C01-1	C01	6.11	SNP2398-SNP37156	3.80	11.86
q2017SH-C01-2		6.02	SNP37161-SNP38210	3.79	11.70	12068324-12309768

位点 Locus	环境 Environment	模型 Model	位点 SNP	染色体 Chr.	位置 Position	阈值 -lg (P)	贡献率 R²(%)
2016SH-1	2016	Naive	S8_12459044	A08	12459044	5.90	15.90
2016SH-2			S10_16428321	A10	16428321	5.62	15.64
2017SH-1	2017	K+PCA	S12_13075515	C02	13075515	7.62	10.08
2017SH-2			S12_13075493	C02	13075493	5.64	6.91
2017SH-3			S16_28288339	C06	28288339	5.70	6.86

Detection of stem height QTL and integration of the loci for plant height- related traits in B. napus

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