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Acta Agronomica Sinica ›› 2021, Vol. 47 ›› Issue (11): 2268-2277.doi: 10.3724/SP.J.1006.2021.04237

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of maize and soybean intercropping on nodule growth, nitrogen fixation of soybean under low phosphorus condition

QIN Xiao-Min1(), PAN Hao-Nan1, XIAO Jing-Xiu1, TANG Li1, ZHENG Yi1,2,*()   

  1. 1Yunnan Agricultural University, Kunming 650201, Yunnan, China
    2Yunnan Open University, Kunming 650599, Yunnan, China
  • Received:2020-10-31 Accepted:2021-03-19 Online:2021-11-12 Published:2021-04-13
  • Contact: ZHENG Yi E-mail:qinxiaomin89@163.com;zhengyi-64@163.com
  • Supported by:
    National Key Research and Development Program of China(2017YFD0200200/207);National Natural Science Foundation of China(31760615);National Natural Science Foundation of China(31760611);National Natural Science Foundation of China(32060718);Science and Technology Talent and Platform of Yunnan Province(2019IC026)

Abstract:

To investigate the effects of maize and soybean intercropping on nitrogen and phosphorus uptake, nodule growth, and nitrogen fixation in soybean, a pot experiment was conducted with two phosphorus (P) rates (low P -P50 and sufficient P -P100). The results showed that, compared with monocropped soybean, intercropping of soybean and maize significantly increased the nodule number, nodule weight, leghemoglobin content, and nitrogenase activity of nodule, and promoted the growth and nitrogen (N) and phosphorus uptake of soybean under P50 and P100 rates. The concentrations of N, P, and the activities of acid phosphatase, phytase in nodules in intercropped soybean were significantly higher than those of monocropped soybean under P50 and P100 rates, and the activities of acid phosphatase and phytase showed the highest values under IS-P50 treatment. In addition, the P concentration in the nodules of intercropped soybean under P50 rate was significantly higher than that of monocropped soybean under P90 rate. so In summary, to maintain the larger phosphorus content for nitrogen fixation of soybean under phosphorus deficiency, the soybean and maize intercropping system increased the phosphorus concentration in the nodules mainly by enhancing the activities of acid phosphatase and phytase in the nodules, and thus promoted the growth and nitrogen uptake of soybean.

Key words: P rates, maize and soybean intercropping, nodule, nitrogen fixation, N and P uptake

Table 1

Effects of maize and soybean intercropping on soybean growth under different P rates (g plant-1)"

磷水平
P rates
种植模式
Planting patterns

Leaf

Stem

Root
全株
Whole plant
P50 MS 4.09±0.20 c 4.05±0.32 c 1.16±0.11 d 9.44±0.13 c
IS 7.04±0.69 b 6.76±0.81 b 2.17±0.15 c 16.23±1.16 b
P100 MS 6.39±0.33 b 6.01±0.27 b 2.48±0.18 b 15.04±0.39 b
IS 8.75±0.44 a 8.27±0.63 a 3.52±0.15 a 20.85±0.76 a

Table 2

Effects of intercropping and P rates on growth and nitrogen fixation ability of soybean nodules"

磷水平
P rates
种植模式
Planting patterns
根瘤数
Nodule number
根瘤干重
Nodule dry weight
豆血红蛋白
Leghemoglobin
固氮酶活性
Nitrogenase activity
P50 29.04 b 0.197 b 4.56 b 1.53 b
P100 42.88 a 0.245 a 5.74 a 2.02 a
MS 24.92 b 0.155 b 4.76 b 1.62 b
IS 47.00 a 0.287 a 5.55 a 1.92 a
磷水平 P rates (P) ** ** ** **
种植模式 Planting patterns (PP) ** ** ** **
P×PP * ns ns ns

Fig. 1

Effects of intercropping on growth, nitrogen fixation ability of soybean nodules under different P rates Abbreviations and treatments are the same as those given in Table 1. Different uppercase and lowercase letters mean significant difference between monocropping and intercropping at the 0.05 probability level under P50 and P100 rates, respectively."

Table 3

Effects of maize and soybean intercropping on N uptake of soybean different organs under different P rates (mg plant-1)"

磷水平
P rates
种植模式
Planting patterns

Leaves

Stems

Roots
根瘤
Nodules
全株
Whole plant
P50 MS 13.76±1.51 a 11.61±1.78 a 1.24±0.09 a 1.22±0.01 a 27.83±1.07 a
IS 25.05±2.46 a 17.31±0.36 a 2.80±0.32 a 2.72±0.19 a 47.88±2.87 a
P100 MS 24.03±1.58 a 21.36±0.61 a 3.41±0.15 a 1.81±0.20 a 50.60±0.97 a
IS 34.63±1.86 a 26.67±2.20 a 5.53±0.24 a 3.59±0.32 a 70.43±3.52 a
施磷量 P rates
P50 19.41 b 14.46 b 2.02 b 1.97 b 37.86 b
P100 29.33 a 24.02 a 4.47 a 2.70 a 60.51 a
种植模式 Planting pattern
MS 18.90 b 16.48 b 2.32 b 1.51 b 39.22 b
IS 29.84 a 21.99 a 4.17 a 3.16 a 59.15 a
显著性 Significance
磷水平 P rates (P) ** ** ** ** **
种植模式 Planting patterns (PP) ** ** ** ** **
P×PP ns ns ns ns ns

Table 4

Effects of maize and soybean intercropping on P uptake of soybean different organs under different P rates (mg plant-1)"

磷水平
P rates
种植模式
Planting patterns

Leaves

Stems

Roots
根瘤
Nodules
全株
Whole plant
P50 MS 12.13±0.56 a 7.66±0.78 a 1.75±0.25 a 1.55±0.07 a 23.09±0.61 a
IS 26.39±2.15 a 15.35±1.91 a 4.46±0.47 a 3.55±0.33 a 49.75±0.57 a
P100 MS 23.74±2.18 a 14.41±0.94 a 5.39±0.67 a 2.17±0.24 a 45.71±3.27 a
IS 37.16±1.54 a 22.36±1.79 a 9.32±0.15 a 4.60±0.22 a 73.45±0.43 a
施磷量 P rates
P50 19.26 b 11.50 b 3.11 b 2.55 b 36.42 b
P100 30.45 a 18.39 a 7.36 a 3.38 a 59.58 a
种植模式 Planting pattern
MS 17.93 b 11.04 b 3.57 b 1.88 b 34.40 b
IS 31.78 a 18.85 a 6.89 a 4.08 a 61.60 a
显著性Significance
磷水平 P rates (P) ** ** ** ** **
种植模式 Planting patterns (PP) ** ** ** ** **
P×PP ns ns * ns ns

Table 5

Effects of intercropping and P rates on N and P concentrations of soybean organs"

磷水平
P rates
种植模式
Planting patterns
氮素浓度N concentrations 磷素浓度P concentrations
叶Leaf 根系Root 根瘤Nodule 叶Leaf 根系Root 根瘤Nodule
P50 34.58 b 11.86 b 98.07 b 3.36 b 1.78 b 12.63 a
P100 38.58 a 14.73 a 107.98 a 3.98 a 2.41 a 13.43 a
MS 35.57 b 12.28 b 96.75 b 3.34 b 1.84 b 11.93 b
IS 37.59 a 14.31 a 109.30 a 4.01 a 2.35 a 14.14 a
磷水平 P rates (P) ** ** * ** ** ns
种植模式 Planting patterns (PP) * * * ** ** **
P×PP ns ns ns ns ns ns

Fig. 2

Effects of maize and soybean intercropping on N concentration of soybean organs Abbreviations and treatments are the same as those given in Table 1. Different uppercase and lowercase letters mean the significant difference between monocropping and intercropping at the 0.05 probability level under P50 and P100 rates, respectively."

Fig. 3

Effects of maize and soybean intercropping on P concentration of soybean organs under different P rates Abbreviations and treatments are the same as those given in Table 1. Different uppercase and lowercase letters mean the significant difference between monocropping and intercropping at the 0.05 probability level under P50 and P100 rates, respectively."

Table 6

Effects of intercropping and P rates on enzyme activities of soybean organs"

磷水平
P rates
种植模式
Planting patterns
酸性磷酸酶Acid phosphatase 植酸酶Phytase
叶Leaf 根系Root 根瘤Nodule 叶Leaf 根系Root 根瘤Nodule
P50 21.19 a 7.74 a 28.89 a 37.17 a 10.00 a 52.84 a
P100 18.37 b 6.38 b 25.35 b 26.67 b 7.80 b 40.56 b
MS 18.48 b 6.22 b 25.60 b 29.45 b 8.18 b 43.67 b
IS 21.07 a 7.90 a 28.64 a 34.40 a 9.62 a 49.73 a
磷水平 P rates (P) ** ** ** ** ** **
种植模式 Planting patterns (PP) ** ** ** ** ** **
P×PP ns ns ns ns ns ns

Fig. 4

Effects of maize and soybean intercropping on enzyme activities of soybean organs under different P rates Abbreviations and treatments are the same as those given in Table 1. Different uppercase and lowercase letters mean significant difference between monocropping and intercropping at the 0.05 probability level under P50 and P100 rates, respectively."

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