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Acta Agronomica Sinica ›› 2021, Vol. 47 ›› Issue (2): 237-244.doi: 10.3724/SP.J.1006.2021.04111

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Distribution survey of soybean cyst nematode of new race X12 in Gujiao city, Shanxi province

LIAN Yun1(), WANG Jin-She1, WEI He1, LI Jin-Ying1, GONG Gui-Ming2, WANG Shu-Feng1, ZHANG Jing-Peng1, LI Mao-Lin1, GUO Jian-Qiu3, LU Wei-Quo1,*()   

  1. 1Institute of Industrial Crops, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plain, Ministry of Agriculture and Rural Affairs / Henan Provincial Key Laboratory for Oil Crops Inprovement, Zhengzhou 450002, Henan, China
    2Gujiao Seed Service Station, Taiyuan 030200, Shanxi, China
    3Luoyang Academy of Agriculture and Forestry Sciences, Luoyang 471023, Henan, China
  • Received:2020-05-18 Accepted:2020-09-13 Online:2021-02-12 Published:2020-09-22
  • Contact: LU Wei-Quo E-mail:lianyun262@126.com;123bean@163.com
  • Supported by:
    Scientific and Technological Project of Henan Province(202102110158);Central Plains Science and Technology Innovation Leading Talent Project(194200510003);National Natural Science Foundation of China(31901501)

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Abstract:

X12 was a new race of soybean cyst nematode (SCN) with super strong pathogenicity and it was first detected in Xingjiashe county, Gujiao city, Shanxi province, China in 2012, which was a huge threat to soybean production. The research of race X12 distribution is meaningful for developing management strategies to prevent race X12 population from spreading. Therefore, a survey for the distribution of soybean cyst nematode race X12 in Gujiao city was conducted in 2019 and 2020. A distribution of races was constructed based on Riggs model. The race distribution was discussed in this study. A total of 33 soil samples infected soybean cyst nematode were collected. Twenty-six were identified as physiological subtypes, accounting for 78.8% of the samples, in which race 2 and race 4 accounted for 57.7% (15 samples) and 42.3% (11 samples), respectively. In general, race X12 was determined if race 4 population virulent on ZDD2315 accession with female index (FI) > 10. In 11 samples determined as race 4, including two samples were further determined as race X12 including the sample collected from Xingjiashe location, which was the original location detected as race X12 in 2012. There were three other SCN populations which determined as race 4 could virulent on ZDD2315 but with FI < 10. There were 73.1% and 57.7% of populations with FI > 50 among 26 evaluated populations on Peking and PI88788, respectively. The results showed that race X12 population were also detected in Hekouzhen except Xingjiashe. Only race 2 and race 4 were detected around Xingjiashe, covered 810 km2. SCN populations with 3 samples were likely to preferentially evolve from subspecies 4 to subspecies X12. The results showed that strong and effective measures should be taken in Gujiao city to slow down the virulence escalation of SCN and the spread of X12 species.

Key words: soybean, soybean cyst nematode, X12, survey, physiological race

Fig. 1

Race distribution of soybean cyst nematode of new race X12 in Gujiao city (surveyed in 2019 and 2020) N: samples infected with SCN but without identification results; R2: race 2; R4: race 4; X12: race X12. Red solid triangle 1 stands for the original race X12 discovery location; red solid triangle 2 stands for determined as race X12 location according to this survey; the bigger blue solid circle stands for race 4 with potential prior determined as race X12 in the future."

Table 1

Virulence phenotypes of twenty-six samples collected from Gujiao city in 2019 and 2020"

小种类型a
Race a (%)		 编号
Number		 Lee上胞囊b
Cysts on Lee b		 胞囊指数Female index
Pickett Peking PI88788 (MU) PI90763 ZDD2315
2号小种(57.7)
Race 2 (57.7)		 1 95 98.9 270.7 85.1 1.9 1.1
4 698 94.8 83.3 26.1 0.5 4.1
6 543 38.9 30.6 33.4 6.4 0.1
8 170 39.6 80.3 91.2 1.4 0.4
9 217 48.0 22.8 26.1 5.1 1.2
11 129 37.7 28.0 84.6 2.7 1.8
12 284 61.7 100.4 58.9 0.5 1.3
16 242 81.1 59.9 60.6 0.2 0.1
18 379 80.9 75.5 31.2 2.6 1.2
19 80 102.4 83.9 63.6 4.2 1.8
20 64 66.7 42.3 40.4 1.3 0.4
24 509 86.4 63.8 98.1 0.4 0.2
25 404 100.7 87.3 69.0 4.5 0.2
29 336 58.7 102.5 32.6 1.0 0.2
31 547 68.3 77.8 87.4 3.7 0.1
X12小种c (7.7)
Race X12 c (7.7)		 1 438 67.6 73.7 36.0 38.0 32.4
2 520 93.3 79.8 84.7 94.1 10.7
小种类型a
Race a (%)		 编号
Number		 Lee上胞囊b
Cysts on Lee b		 胞囊指数Female index
Pickett Peking PI88788 (MU) PI90763 ZDD2315
潜在的X12小种(11.5)
Potential race X12 (11.5)
 5 258.6 173.9 86.1 69.8 39.0 7.6
21 479 102.7 80.5 61.0 13.6 9.6
27 249 69.3 62.9 32.3 12.2 6.4
4号小种(23.1)
Race 4 (23.1)
 10 298 91.6 61.5 55.6 15.2 1.1
14 550 37.2 69.5 73.3 17.7 0.4
17 706 86.2 77.2 82.1 72.7 0.8
26 815 84.7 82.9 55.2 12.2 0.1
28 251 53.3 147.5 75.6 16.0 1.8
33 633 91.5 90.8 73.5 34.7 0.4

Table 2

Samples of different physiological subtypes collected from GuJiao city with HG type in 2019 and 2020"

项目
Item		 寄主
Host		 编号 Number
16 14 21 30
Lee上胞囊数
Number of cysts on Lee		 482.7 156.0 787.0 340.7
胞囊指数
Female index		 Peking 45.5 69.7 65.9 79.8
PI88788 53.0 105.6 61.2 76.1
PI90763 3.5 18.2 14.4 12.7
PI437654 2.3 3.6 0.8 105.0
PI209332 15.8 50.3 14.4 87.4
PI89772 44.7 102.4 21.3 141.1
PI548316 70.0 81.8 12.5 161.7
小种类型
Race		 Race model Race 2 Race 4 Potential X12 Race X12
HG type 1.2.5.6.7 1.2.3.5.6.7 1.2.3.5.6.7 1.2.3.4.5.6.7
[1] Allen T W, Bradley C A, Sisson A J, Byamukama E, Chilvers M I, Coker C M, Collins A A, Damicone J P, Dorrance A E, Dufault N S, Esker P D, Faske T R, Giesler L J, Grybauskas A P, Hershman D E, Hollier C A, Isakeit T, Jardine D J, Kelly H M, Kemerait R C, Kleczewski N M, Koenning S R, Kurle J E, Malvick D K, Markell S G, Mehl H L, Mueller D S, Mueller J D, Mulrooney R P, Nelson B D, Newman M A, Osbome L, Overstreet C, Padgett G B, Phipps P M, Price P P, Sikora E J, Smith D L, Spurlock T N, Tande C A, Tenuta A U, Wise K A, Wrather J A. Soybean yield loss estimates due to diseases in the United States and Ontario, Canada, from 2010 to 2014. Plant Health Prog, 2017,18:19-27.
[2] Koenning S R, Wrather J A. Suppression of soybean yield potential in the continental United States by plant diseases from 2006 to 2009. Plant Health Prog, 2010. doi: org/10.1094/PHP-2010- 1122-01-RS.
pmid: 10275108
[3] Lian Y, Guo J Q, Li H C, Wu Y K, Wei H, Wang J S, Li J Y, Lu W G. A new race (X12) of soybean cyst nematode in China. J Nematol, 2017,49:321-326.
[4] 练云, 王金社, 李海朝, 魏荷, 李金英, 武永康, 雷晨芳, 张辉, 王树峰, 郭建秋, 李月霞, 李志辉, 靳巧玲, 徐淑霞, 张志民, 杨采云, 于会勇, 耿臻, 舒文涛, 卢为国. 黄淮大豆主产区大豆胞囊线虫生理小种分布调查. 作物学报, 2016,42:1479-1486.
Lian Y, Wang J S, Li H C, Wei H, Li J Y, Wu Y K, Lei C F, Zhang H, Wang S F, Guo J Q, Li Y X, Li Z H, Jin Q L, Xu S X, Zhang Z M, Yang C Y, Yu H Y, Geng Z, Shu W T, Lu W G. Race distribution of soybean cyst nematode in the main soybean producing area of Huang-Huai Rivers valley. Acta Agron Sin, 2016,42:1479-1486 (in Chinese with English abstract).
[5] Riggs R D, Schmitt D P. Complete characterization of the race scheme for Heterodera glycines. J Nematol, 1988,20:392-395.
pmid: 19290228
[6] Niblack T L, Arelli P R, Noel G R, Opperman C H, Orf J H, Schmitt D P, Shannon J G, Tylka G L. A revised classification scheme for genetically diverse populations of Heterodera glycines. J Nematol, 2002,34:279-288.
pmid: 19265945
[7] 林汉明, 常汝镇, 邵桂花, 刘忠堂. 中国大豆耐逆研究. 北京: 中国农业出版社, 2009. pp 155-158.
Lin H M, Chang R Z, Shao G H, Liu Z T. Research on Tolerance to Stresses in Chinese Soybean. Beijing: China Agriculture Press, 2009. pp 155-158(in Chinese).
[8] 大豆种质抗胞囊线虫鉴定协作组. 大豆种质资源对大豆胞囊线虫1、3和4号生理小种的抗性鉴定. 大豆科学, 1993,12(2):91-99.
Identification cooperation group of soybean germplasms with resistance to soybean cyst nematode. Resistance evaluation of soybean germplasm to race1, 3 and 4 of soybean cyst nematode (Heterodera glycines). Soybean Sci, 1993,12(2):91-99 (in Chinese with English abstract).
[9] Kadama S, Vunoga T D, Qiua D, Meinhardt C G, Song L, Deshmukh R, Patil G, Wan J, Valliyodan B, Scaboo A M, Shannon J G, Nguyen H T. Genomic-assisted phylogenetic analysis and marker development for next generation soybean cyst nematode resistance breeding. Plant Sci, 2016,242:342-350.
pmid: 26566850
[10] 卢为国, 盖钧镒, 李卫东. 黄淮地区大豆胞囊线虫生理小种的抽样调查与研究. 中国农业科学, 2006,39:306-312.
Lu W G, Gai J Y, Li W D. Sampling survey and identification of races of soybean cyst nematode (Heterodera glycines Ichinohe) in Huang-Huai valleys. Sci Agric Sin, 2006,39:306-312 (in Chinese with English abstract)
[11] Mitchum M G, Wrather J A, Heinz R D, Shannon J G, Danekas G. Variability in distribution and virulence phenotypes of Heterodera glycines in Missouri during 2005. Plant Dis, 2007,91:1473-1476.
doi: 10.1094/PDIS-91-11-1473 pmid: 30780744
[12] Howland A, Monnig N, Mathesius J, Nathan M, Mitchum M G. Survey of Heterodera glycines population densities and virulence phenotypes during 2015-2016 in Missouri. Plant Dis, 2018,102:2407-2410.
[13] Niblack T L, Colgrove K B, Colgrove A C. Soybean cyst nematode in Illinois from 1990 to 2006: shift in virulence phenotype of field populations. J Nematol, 2006,38:285.
[14] Tylka G L, Marrett C C. Distribution of the soybean cyst nematode, Heterodera glycines, in the United States and Canada: 1954-2014. Plant Health Prog, 2014,15:13-15.
[15] Mitchum M G. Soybean resistance to the soybean cyst nematode Heterodera glycines: an update. Phytopathology, 2016,106:1444-1450.
[16] Peng D L, Peng H, Wu D Q, Huang W, Cui J K. First report of soybean cyst nematode (Heterodera glycines) on soybean from Gansu and Ningxia China. Plant Dis, 2015,100:229.
[17] Wang D, Duan Y X, Wang Y Y, Zhu X F, Chen L J, Liu X Y. First report of soybean cyst nematode, Heterodera glycines, on soybean from Guangxi, Guizhou, and Jiangxi provinces, China. Plant Dis, 2015,99:893.
[18] 王金社, 卢为国, 李金英, 练云, 魏荷, 李海朝, 雷晨芳. 专利号: 2014SR060158, 植物病虫害表型数据采集系统. 中国, 2014.
Wang J S, Lu W G, Li J Y, Lian Y, Wei H, Li H C, Lei C F. The data acquisition system on the phenotype of plant diseases and insect pests. Patent number: 2014SR060158, China, 2014 (in Chinese).
[19] The R Core Team. R: a Language and Environment for Statistical Computing. R Foundation for Statistical Computing. Vienna, Austria, 2005. https://cran.r-project.org/doc/manuals/ fullrefman.pdf.
[20] Niblack T L, Colgrove A L, Colgrove K, Bond J P. Shift in virulence of soybean cyst nematode is associated with use of resistance from PI88788. Plant Health Prog, 2008,29. doi: org/10. 1094/PHP-2008-0118-01-RS
[21] Hua C, Li C, Hu Y, Mao Y, You J, Wang M, Chen J, Tian Z, Wang C. Identification of HG types of soybean cyst nematode Heterodera glycines and resistance screening on soybean genotypes in Northeast China. J Nematol, 2018,50:41-50.
pmid: 30335911
[22] Concibido V C, Diers B W, Arelli P R. A decade of QTL mapping for cyst nematode resistance in soybean. Crop Sci, 2004,44:1121-1131.
doi: 10.2135/cropsci2004.1121
[23] McCarville M T, Marett C C, Mullaney M P, Gebhart G D, Tylka G L. Increase in soybean cyst nematode virulence and reproduction on resistant soybean varieties in Iowa from 2001 to 2015 and the effects on soybean yields. Plant Health Prog, 2017,18:146-155.
[24] Gardner M, Heinz R, Wang J, Mitchum M G. Genetics and adaptation of soybean cyst nematode to broad spectrum soybean resistance. G3: Genes Genom Genet, 2017,7:835-841.
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