Abstract:

It is a valuable way to develop co-segregation molecular markers for enhancing selection efficiency. Using maize(Zea mays L.) resistant inbred line P₂₅, susceptible inbred line F₃₄₉ and the derived NILs of BC₅(F₃₄₉ is the recurrent parent) to southern corn rust as materials, a 321-bp specific DNA band was amplified and cloned by means of RGA (resistance gene analog) primers. The new primers designed in terms of the above band sequence as a molecular marker were used for marker assistant selection(MAS) among the 96 BC₇, BC₄F₅ inbreds and their parents in order to evaluate the selection efficiency. Finally, the specific band was gained and the performance in 94 of 96 plants was consistent between the field test and molecular marker analysis. The selection efficiency of the marker was up to 97.9%. The marker can produce clear and steady amplification bands in PCR, and be used valuably in MAS for southern corn rust resistance.

Key words: Southern Corn Rust, RGA, Molecular Marker, Maize (Zea mays L.)