Abstract:

Improving anther culture inductivity is important for improvements of plant differentiation rate and plant production rate in rice. Four pedigree seed varieties of autotetraploid rice, Linglun, 02428, Pei’ai 64 and Lunhui 422 and the corresponding diploid plants were used in the present work of anther culture. 26 kinds of induction medium were designed on the factors including agar concentration (0.5%, 0.75% and 1%), variety (sucrose and maltose) and concentration (60 and 80 g L^-1) of carbon source, different varieties of basic media (MS, N6, M8, NB) and hormone (KT and Zip) to determine appropriate combination on callus induction. The results indicated that the inductivity of 60 g L^-1 (18.7%) is notably better than that of 80 g/L (8.7%) when using sucrose as carbon source. Meanwhile, using 0.75% of agar obtained the highest inductivity which is 20.8% among twelve media combination, and the average induction rate of autotetraploid japonica (16.4%) is higher than that of autotetraploid indica (7.3%). The N6 and NB media were better than MS and M8 for improving callus inductivity in tetraploid rice, especially in Japonica rice, whether sucrose or maltose was used as carbon source. The media with supplement of KT and Zip could induce more calli conspicuously, and the highest inductivity was 84%. It was also observed that the calli induced by the addition of KT were of high quality to produce seedlings. Among total 26 kinds of media, 6 types led to higher inductivity in japonica rice whereas 2 types in indica rice. Furthermore, there were 3 types media produced not only higher callus inductivity but also higher plant production rate than others. In conclusion, the induction efficiency of anther callus for one certain genotype is remarkably different with various media, which implies that the genotype is of selection for the induction media.

Key words: Rice, Tetraploid, Anther culture, Efficiency of callus induction