Abstract:

Fertility restoration of wheat male sterile line with Ae. kotschyi cytoplasm is one of main factors affecting its application in production, and the study of its genetic mechanism is helpful to develop good restorer lines. The sterile lines Yumai 3, S43 and their corresponding maintainer lines, the restorer lines Yumai 2 and Yumai 49, and three kinds of BC1 populations sterile line//maintainer line/restorer line, sterile line // sterile line/restoring line and sterile line / restoring line//maintainer line, were used to study the inheritance of fertility restoring genes. The results showed that the restorer line with different restoring ability had different number of restoring genes. Fertility segregation of backcross populations revealed two pairs of major restoring genes in restoring line Yumai 2, and only one pairs of genes in restoring line Yumai 49(Table 2), which was the main reason that the hybrids with Ae. kotschyi cytoplasm using Yumai 2 had higher restoring degree than that using Yumai 49. Besides, fertility restoration was affected by some quantitative genes such as enhancing or inhibiting genes, which were found not only in restoring lines but also in sterile lines (maintainer lines) (Table 5). The inheritance of female gamete without any restoring gene was normal under Ae. kotschyi cytoplasm background, but the transmission rate of male gamete was very low(Table 3 and Table 4). According to other author’s results, whether the wheat male sterile line with Ae.Kotschyi cytoplasm is completely sterile from gamete cannot be proved.

Key words: Wheat, Male sterile line with Ae. kotschyi cytoplasm, Restoring gene, Inheritance, Gamete transmission rate